Saliva and Serum Levels of B‐Cell Activating Factors and Tumor Necrosis Factor‐α in Patients With Periodontitis

Gümüş, Pınar; Nizam, Nejat; Lappin, David F.; Buduneli, Nurcan

doi:10.1902/jop.2013.130117

Cited by 91 publications

(70 citation statements)

References 56 publications

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“…However, inclusion of both current and former smokers may be regarded as a limitation of the present study, as smoking can affect both salivary and serum cytokine levels [24]. On the other hand, the numbers of current/former smokers appeared to be balanced in the study groups and similar effects of smoking might be expected on the biochemical measurements in each study group.…”

Section: Discussionmentioning

confidence: 85%

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Is there an association between obstructive sleep apnea syndrome and periodontal inflammation?

et al. 2015

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Objectives: To assess salivary, serum biomarkers, and subgingival bacteria as putative candidates in the potential association between obstructive sleep apnea syndrome (OSAS) and periodontal disease. Materials and methods:Fifty-two patients were grouped according to the severity of OSAS: 13 participants served as controls, 17 patients had mild-to-moderate OSAS, and 22 severe OSAS. Serum, saliva, and subgingival plaque samples were collected, clinical periodontal parameters recorded. Salivary, serum concentrations of interleukin-6 (IL-6), tumour necrosis factor (TNF-α), osteoprotegerin, sRANKL, and apelin were analysed by enzyme-linked immunosorbent assay. Bacterial counts were determined by real-time QPCR on plaque microbial DNA preparations.Results: There was a significant change in the composition of microbes in plaque particularly in severe OSAS samples (p<0.01). Statistical analyses indicated significantly higher salivary IL-6 levels in both OSAS groups compared to controls (p<0.05). Salivary apelin levels were significantly higher in Serum levels of these biomarkers and salivary osteoprotegerin, sRANKL levels were similar in the study groups. The incidence and duration of apnea positively correlated with clinical periodontal parameters (p<0.05). Conclusion:OSAS appeared to alter the tested bacteria in plaque, correlate with increasing periodontal disease severity, have additive effect on salivary IL-6.Clinical relevance: OSAS is likely to interact with periodontal disease.

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Section: Discussionmentioning

confidence: 85%

“…Increased levels of inflammatory markers have been proposed to have a role in periodontal disease and on interactions with systemic diseases [23,24].…”

Section: Introductionmentioning

confidence: 99%

Is there an association between obstructive sleep apnea syndrome and periodontal inflammation?

et al. 2015

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“…Thus, it is likely that APRIL detected in the gingival epithelium was locally produced in response to dental plaque bacteria. An additional possibility is that APRIL might be in part systemically derived, although the serum levels of APRIL in patients with chronic periodontitis are not significantly higher than those of healthy controls (44). The APRIL-rich zone within the gingiva was similar to that previously described in mucosa-associated lymphoid tissues (24).…”

Section: Discussionmentioning

confidence: 99%

“…A recent cross-sectional study reported on the presence of APRIL and BLyS in the serum and saliva of patients with chronic periodontitis and healthy controls (44). Although BLyS was detected at significantly higher concentrations in both fluids from patients relative to controls, there were no statistically significant differences in the levels of APRIL between the two groups (44).…”

Section: Discussionmentioning

confidence: 99%

The B Cell–Stimulatory Cytokines BLyS and APRIL Are Elevated in Human Periodontitis and Are Required for B Cell–Dependent Bone Loss in Experimental Murine Periodontitis

Abe

AlSarhan

Benakanakere

et al. 2015

The Journal of Immunology

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B-lineage cells (B lymphocytes and plasma cells) predominate in the inflammatory infiltrate of human chronic periodontitis. However, their role in disease pathogenesis and the factors responsible for their persistence in chronic lesions are poorly understood. In this regard, two cytokines of the TNF ligand superfamily, namely a proliferation-inducing ligand (APRIL) and B-lymphocyte stimulator (BLyS), are important for the survival, proliferation, and maturation of B cells. We thus hypothesized that APRIL and/or BLyS are upregulated in periodontitis and contribute to induction of periodontal bone loss. This hypothesis was addressed in both human and mouse experimental systems. We show that, relative to healthy controls, the expression of APRIL and BLyS mRNA and protein was upregulated in natural and experimental periodontitis in humans and mice, respectively. The elevated expression of these cytokines correlated with increased numbers of B cells/plasma cells in both species. Moreover, APRIL and BLyS partially colocalized with kappa light chain-expressing B lineage cells at the epithelial-connective tissue interface. Ligature-induced periodontitis resulted in significantly less bone loss in B cell-deficient mice compared to wild-type controls. Ab-mediated neutralization of APRIL or BLyS diminished the number of B cells in the gingival tissue and inhibited bone loss in wild-type but not in B cell-deficient mice. In conclusion, B cells and specific cytokines involved in their growth and differentiation contribute to periodontal bone loss. Moreover, APRIL and BLyS have been identified as potential therapeutic targets in periodontitis.

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“…Furthermore, a strong relationship was found among serum levels of IL-32 and the gingival IL-32 levels and the serum levels of IL-6, as well as the alveolar bone and attachment loss. It was previously demonstrated that IL-32 was considerably upregulated by the periodontal pathogens, P.gingivalis and Fusobacterium nucleatum, in an oral epithelial cell line H400 (30). Moreover, IL-32 was expressed by monocytes via P.gingivalis-derived LPS and supposedly IL-32 is a factor that leads to the formation of periodontal disease (15).…”

Section: Discussionmentioning

confidence: 98%

Evaluation of IL-32 levels in gingival tissue and serum of experimental periodontitis model

Dede¹,

Ballı²,

Durmuşlar³

et al. 2017

J Turgut Ozal Med Cent

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Aim: Interleukin (IL)-32, a recently discovered proinflammatory cytokine, is demonstrated in several infectious diseases. The goal of this study is to investigate the levels of IL-6, IL-10 and IL-32 in gingival tissue and serum of rats with experimental periodontitis. Material and Methods: Experimental periodontitis was induced by placing a silk ligature around the cervix of both sides of mandibular first molars in each male rat except for control group (Group 1). Thirty male Wistar albino rats were randomly divided into three groups of ten animals each as experimental periodontitis groups (Group 2, ligated for seven days; Group 3, ligated for fourteen days) and periodontally healthy control group (Group 1). At the end of experimental period, rats were sacrificed, and histomorphometric analyses were performed on the mandibles. IL-32, IL-10 and IL-6 levels were measured in gingival tissue and serum samples by ELISA. Results: Alveolar bone and attachment loss were statistically higher in all experimental groups than those in control group (P<0.001). It was found that the levels of IL-32 and IL-6 (P<0.01) and IL-10 (P<0.05) in gingival tissues were higher in Groups 2 and 3 than those in Group 1 except for IL-32 and IL-10 levels in Group 3. There was a positive correlation between levels of IL-32 and IL-6 in the serum and gingival tissues in all groups (P<0.05). Conclusion:The present results reveal that IL-32 values are locally increased in periodontitis. Proinflammatory cytokines properties, which are linked to periodontal tissue destruction, are also associated with IL-32.

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Saliva and Serum Levels of B‐Cell Activating Factors and Tumor Necrosis Factor‐α in Patients With Periodontitis

Cited by 91 publications

References 56 publications

Is there an association between obstructive sleep apnea syndrome and periodontal inflammation?

Is there an association between obstructive sleep apnea syndrome and periodontal inflammation?

The B Cell–Stimulatory Cytokines BLyS and APRIL Are Elevated in Human Periodontitis and Are Required for B Cell–Dependent Bone Loss in Experimental Murine Periodontitis

Evaluation of IL-32 levels in gingival tissue and serum of experimental periodontitis model

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