Salinimonas lutimaris sp. nov., a polysaccharide-degrading bacterium isolated from a tidal flat

Abstract

A Gram-negative, non-motile, non-endospore-forming bacterial strain, designated DPSR-4(T), was isolated from a tidal flat sediment on the southern coast of Korea. Strain DPSR-4(T) grew optimally at 25-30°C, at pH 7.0-7.5 and in the presence of 2% (w/v) NaCl. A Neighbour-Joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain DPSR-4(T) clustered with Salinimonas chungwhensis BH030046(T) by a high bootstrap resampling value of 99.7%. Strain DPSR-4(T) exhibited 96.2% 16S rRNA gene sequence similarity to that of S. chungwhensis BH030046(T) and 93.7-96.6% sequence similarity to the sequences of type strains of Alteromonas species. Strain DPSR-4(T) contained Q-8 as the predominant ubiquinone and iso-C(15:0) 2-OH and/or C(16:1) ω7c, C(16:0) and C(18:1) ω7c as the major fatty acids. The major polar lipids detected in strain DPSR-4(T) and S. chungwhensis KCTC 12239(T) were phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content was 53.4 mol%. Differential phenotypic properties and phylogenetic distinctiveness of strain DPSR-4(T) demonstrated that this strain is distinguishable from the sole recognized species of the genus Salinimonas, S. chungwhensis. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DPSR-4(T) is considered to represent a novel species of the genus Salinimonas, for which the name Salinimonas lutimaris sp. nov. is proposed. The type strain is DPSR-4(T) (KCTC 23464(T), CCUG 60743(T)).

Bowmanella dokdonensis sp. nov., a novel exoelectrogenic bacterium isolated from the seawater of Dokdo, Korea

“…Ubiquinone Q-8 was only detected as the predominant isoprenoid quinone in the species of the genera Aestuariibacter (Jean et al 2013;Yi et al 2004) and Alteromonas (Ivanova et al 2000(Ivanova et al , 2005Martínez-Checa et al 2005;Yoon et al 2003a), Glaciecola (Jean et al 2013) and Salinimonas (Jeon et al 2005;Yoon et al 2012). The common polar lipids of the species in the genus Bowmanella were reported to be phosphatidylglycerol and phosphatidylethanolamine.…”

Pseudobowmanella zhangzhouensis gen. nov., sp. nov., isolated from the surface freshwater of the Jiulong River in China

“…The major fatty acids ([10 % of total fatty acids) of the strain JS7-9 T were determined as C 16:0 (21.7 %), Sum In Feature 3 (C 16:1 x7c/x6c) (16.9 %) and Sum In Feature 8 (C 18:1 x7c/x6c) (14.9 %) which are characteristic fatty acids for other closely-related genera such as Bowmanella (Jean et al 2006;Lai et al 2009b), Aestuariibacter (Yi et al 2004;Tanaka et al 2010;Wang et al 2010), Aliiglaciecola (Chen et al 2009a, b;Tanaka et al 2010), Alteromonas (Raguenes et al 1996;Yan et al 2011) andSalinimonas (Jeon et al 2005;Yoon et al 2012; Table 1). The substantial proportion (more than 10 % of total fatty acids) of Sum In Feature 8 (C 18:1 x7c/x6c) determined in strain JS7-9 T can be used as a differential feature of the newly isolated bacterium and those of the genera Catenovulum (Yan et al 2011) and Glaciecola (Baik et al 2006;Chen et al 2009a;Park and Yoon 2013).…”

Winogradskyella multivorans sp. nov., a polysaccharide-degrading bacterium isolated from seawater of an oyster farm

“…The artificial seawater contained (l -1 distilled water) 23.6 g NaCl, 0.64 g KCl, 4.53 g MgCl 2 Á6H 2 O, 5.94 g MgSO 4 Á7H 2 O and 1.3 g CaCl 2 Á2H 2 O (Bruns et al 2001). Degradation of various polysaccharides was tested as described previously (Yoon et al 2012). Acid production from carbohydrates was determined as described by Leifson (1963).…”

“…Gaps at the 5 0 and 3 0 ends of the alignment were omitted from further analysis. Phylogenetic analysis was performed as described by Yoon et al (2012).…”