Bowmanella dokdonensis sp. nov., a novel exoelectrogenic bacterium isolated from the seawater of Dokdo, Korea

Abstract

A Gram-negative, motile, and rod-shaped bacterial strain, UDC354(T), was isolated from the seawater of Dokdo, Korea. The strain UDC354(T) displayed optimal growth at 30-37 °C in the presence of 0-2 % (w/v) NaCl at pH 8. Strain UDC354(T) was found to contain Q-8 and 9 as isoprenoid ubiquinones; C16:0 (22.9 %), summed feature 3 (iso-C15:0 2-OH and C16:1 ω7c) (21.4 %) and C18:1 ω7c (12.2 %) as the major fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as the major polar lipids. The DNA G+C content was found to be 54.1 mol %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain UDC354(T) belongs to the genus Bowmanella, of the family Alteromonadaceae, and is closely related to Bowmanella pacifica W3-3A(T) (95.2 %) and Bowmanella denitrificans BD1(T) (95.0 %). It was found that strain UDC354(T) is exoelectrogenic and is capable of generating 6.6 μW/cm(3) in marine broth in the microbial fuel cells. Based on the analysis of the phenotypic, chemotypic and phylogenetic characteristics, a new species, Bowmanella dokdonensis sp. nov. is proposed. The type strain is UDC354(T) (=KCTC 42147(T)=JCM 30855(T)).

Bowmanella yangjiangensis sp. nov. and Amphritea pacifica sp. nov., isolated from mariculture fishponds in China

“…The polar lipids of strains Y26 and Y57 T consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol, that was similar to the polar lipid profiles of other members within the genus Bowmanella (Fig. S5) [1–3]. Strain Y26 contained one more unidentified phospholipid than strain Y57 T .…”

“…The genus Bowmanella is a member of the family Alteromonadaceae which belongs to the class Gammaproteobacteria [1]. Only three validly species of this genus are described at present, including Bowmanella denitrificans BD1 T , Bowmanella pacifica W3-3A T and Bowmanella dokdonensis UDC354 T [1][2][3]. The genus Amphritea proposed by Gärtner et al in 2008 belongs to the family Oceanospirillaceae within the class Gammaproteobacteria and takes Amphritea atlantica M41 T as type species [4].…”

“…The predominant respiratory lipoquinone of the genera Bowmanella and Amphritea is Q-8 and the major phospholipids are phosphatidylethanolamine and phosphatidylglycerol. The major fatty acid compositions of both genera comprise C 16 : 1 ω7c, C 16 : 0 and C 18 : 1 ω7c [1][2][3][4][5][6][7][8]. During a microbial resource survey of mariculture fishponds located in the coastal area of Guangdong Province in PR China, strains Y26, Y57 T , ZJ14W T and RP18W were isolated.…”

“…During isolation, 200 µl water sample was spread onto the agar plate. The medium used for isolating strains Y26 and Y57 T contained C 4 H 4 Na 2 O 4 (1 g l −1 ), KNO 3 (1 g l −1 ), NaCl (15 g l −1 ), MgSO 4 (3 g l −1 ), MgCl 2 (2 g l −1 ), CaCl 2 (1 g l −1 ), KCl (0.7 g l −1 ), NaHCO 3 (0.2 g l −1 ), K 2 HPO 4 (0.1 g l −1 ) and agar (20 g l −1 ) with an adjusted pH of 7.5. The medium used for isolating strains ZJ14W T and RP18W contained yeast extract (0.5 g l −1 ), tryptone (0.5 g l −1 ), KCl (2 g l −1 ), NaCl (15 g l −1 ), MgSO 4 •7H 2 O (5 g l −1 ) and agar (20 g l −1 ) with an adjusted pH of 7.5.…”

Paraneptunicella aestuarii gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from seawater in East China Sea

“…Strains: 1, NBU2194 T ; 2, N. marina S27-2 T [31]; 3, Bowmanella [32]; 4, Alteromonas [33][34][35]; 5, Aestuariibacter [36,37]; 6, Aliiglaciecola [4,38,39]; 7, Marisediminitalea [3]. Tests for hydrolysis of casein, starch, protein, Tweens 20, 40, 60 and 80, the API 20E, API 20NE and API ZYM strips and of fatty acids for strain NBU2194 T and N. marina S27-2 T were examined in this study.…”