Saline-soluble preparations of deoxyribonucleoproteins

Atchley, William A.; Bhagavan, N.V.

doi:10.1016/0003-9861(64)90222-x

Cited by 25 publications

(6 citation statements)

References 17 publications

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“…As can be seen, our data are in good agreement with the data of 3 Table, the contents of triglycerides and fatty acids fluctuate over a comparatively wide range. On the other hand, the amounts of cholesterol and cholesterol esters are very constant .…”

Section: Resultssupporting

confidence: 91%

“…In Table II some phospholipid data for different kinds of rat liver membranes are listed together with those of the present study. As can be seen, our data are in good agreement with the data of 3 , the contents of triglycerides and fatty acids fluctuate over a comparatively wide range. On the other hand, the amounts of cholesterol and cholesterol esters are very constant .…”

Section: Resultssupporting

confidence: 91%

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Nuclear Membranes From Mammalian Liver

Kleinig

1970

The Journal of Cell Biology

112

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The qualitative and quantitative lipid composition of nuclei and nuclear membranes from pig and rat liver were determined . These determinations were compared with the corresponding data obtained for microsomes from the same material after similar treatments . The results indicate that, at least, by far the major part of the nuclear lipids is located in the membranes of the nuclear envelope . The phospholipid pattern of the nuclear membranes and the endoplasmic reticulum (ER) membranes in general is widely identical in both species . As a striking difference in the lipid composition, however, a fourfold increase of esterified cholesterol in the nuclear membranes was found . In a quantitative approach the ratio of total surface area of the nuclear lipids to the total surface area of the nuclear envelope membranes was calculated as being 3 .6, a value which fairly approximates the requirements of a bimolecular lipid leaflet model .

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 91%

Nuclear Membranes From Mammalian Liver

Kleinig

1970

The Journal of Cell Biology

112

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“…The effect of dielectric constant change on the DNA precipitation is also found in the case of chromatin precipitation. Indeed, Atchley and Bhagavan (1964), who studied the aminocarboxylic acids effects on the solubility of crude deoxyribonucleoprotein (DNP) extruded from malignant mammalian cells, have observed that the addition of glycine, 13-alanine, 4-aminobutyric acid, and 6-aminocaproic acid significantly increased the solubility of DNP in 0.01 MgCl2. By a careful analysis of their results, we can see that the same percentage inhibition of precipitation was obtained for the same dielectric constant in solution: 15% and 12% in V=1286.5 cm /mole 3 V=643.3 cm /mole 3 V=321.6 cm /mole the presence of 1.2 M glycine (Er = 107) and 0.8 M f3-alanine (Er = 108), respectively; 23% and 25% in the presence of 1.6 M glycine (Er = 116) and 1.2 M B3-alanine (Er = 122); 82% and 80% in the presence of 1.2 M y-aminobutyric acid (Er = 141) and 0.8 M E-aminocaproic acid (Er = 142).…”

Section: Possibility Of Control Of the Osmotic Stress By A Variation mentioning

confidence: 99%

Dielectric constant and ionic strength effects on DNA precipitation

Flock¹,

Labarbe²,

Houssier³

1996

Biophysical Journal

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We have investigated the effect of different zwitterionic compounds on DNA precipitation induced by spermine4+. Glycine, beta-alanine, 4-aminobutyric acid, and 6-aminocaproic acid have shown an increasing capacity to attenuate DNA precipitation. This protection effect has been correlated with the dielectric constant increase of their corresponding solutions. Calculations based on these experimental data and counter-ion condensation theory have confirmed the importance of this parameter for DNA-ion interactions and precipitation mechanisms. We have also observed a resolubilization of DNA in the presence of 6-aminocaproic acid at high spermine4+ concentration and in the presence of glycine at high spermidine3+ concentration. This could be explained by an increase of screening effect with polyamine concentration.

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“…Since the discovery of tbe important role of histones in gene regulation and cellular differentiation (Huang and Bonner 1962, Allfrey and Mirsky 1962, AUfrey et al 1963, Allfrey et al 1964, Bonner and Huang 1964, tbe investigations bave been concerned with the isolation and characterization of deoxyribonucleoprotein complexes from living tissues (Frenster et al 1963, Atchley and Bhagavan 1964, Jellum and Eldjam 1965, Sekigucbi et al 1967. It was previously proven that, as with the cellular differentiation, biochemical and enzymatic alterations occur during the conjugation process of Spirogyra (Sasaki et al 1966, Yamashita et al 1968, Sasaki et al 1972, Sasaki and Takaya 1972.…”

Section: Introductionmentioning

confidence: 99%

Thermal Denaturation Properties of Deoxyribonucleic Acid and Chromatin during the Life Cycle of Spirogyra

Sasaki

Takaya

1974

Physiologia Plantarum

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Half melting temperature (Tm) of DNA prepared from the nuclear fraction of vegetatively growing cells of Spirogyra was 86°C in the standard saline‐citrate solution and 68°C in the dilute saline‐citrate solution, and the major Tm values of soluble chromatin were 68°C and 78–80°C in the dilute saline‐citrate solution. The Tm value of DNA prepared from the nuclear fractions of conjugating and zygote cells was 88°C in the standard saline‐citrate solution and 70°C in the dilute saline‐citrate solution, and the major Tm values of soluble chromatin were 72°C, 78–80°C, and 84°C in the dilute saline‐citrate solution. The Tm values of DNA prepared from cells arrested at different stages of the life cycle of Spirogyra were raised by combining with different fractions of histones. The results agreed with an altered structure of the nucleoprotein complex during the life cycle of Spirogyra.

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Saline-soluble preparations of deoxyribonucleoproteins

Cited by 25 publications

References 17 publications

Nuclear Membranes From Mammalian Liver

Nuclear Membranes From Mammalian Liver

Dielectric constant and ionic strength effects on DNA precipitation

Thermal Denaturation Properties of Deoxyribonucleic Acid and Chromatin during the Life Cycle of Spirogyra

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