Salicylic Acid Reduces Napropamide Toxicity by Preventing Its Accumulation in Rapeseed (Brassica napus L.)

Abstract

Napropamide is a widely used herbicide for controlling weeds in crop production. However, extensive use of the herbicide has led to its accumulation in ecosystems, thus causing toxicity to crops and reducing crop production and quality. Salicylic acid (SA) plays multiple roles in regulating plant adaptive responses to biotic and environmental stresses. However, whether SA regulates plant response to herbicides (or pesticides) was unknown. In this study, we investigated the effect of SA on herbicide napropamide accumulation and biological processes in rapeseed (Brassica napus). Plants exposed to 8 mg kg(-1) napropamide showed growth stunt and oxidative damage. Treatment with 0.1 mM SA improved growth and reduced napropamide levels in plants. Treatment with SA also decreased the abundance of O (2) (-.) and H(2)O(2) as well as activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and increased activities of guaiacol peroxidase (POD) and glutathione-S-transferase (GST) in napropamide-exposed plants. Analysis of SOD, CAT, and POD activities using nondenaturing polyacrylamide gel electrophoresis (PAGE) confirmed the results. These results may help to understand how SA regulates plant response to organic contaminants and provide a basis to control herbicide/pesticide contamination in crop production.

A collection of cytochrome P450 monooxygenase genes involved in modification and detoxification of herbicide atrazine in rice (Oryza sativa) plants

“…salicylic acid, abscisic acid, gibberellins, jasmonic acid, and brassinosteroids) and resistance to toxic compounds (Jensen and Møller, 2010;Mizutani and Ohta, 2010;Cui et al, 2010),. For example, salicylic acid (SA), a regulator of plant response to various environmental stresses (Yang et al, 2003;Liang et al, 2012), has been reported to improve plant resistance to herbicide napropamide toxicity in rapeseed (Brassica napus) (Cui et al, 2010). Cytochrome P450s are known to be involved in detoxification of xenobiotics in the phase I metabolism in plants (Siminszky, 2006;Zhang et al, 2014;Kebeish et al, 2014).…”

“…phenylpropanoids, alkaloids, terpenoids, lipids, cyanogenic glycosides, glucosinolates), growth regulation (e.g. salicylic acid, abscisic acid, gibberellins, jasmonic acid, and brassinosteroids) and resistance to toxic compounds (Jensen and Møller, 2010;Mizutani and Ohta, 2010;Cui et al, 2010),. For example, salicylic acid (SA), a regulator of plant response to various environmental stresses (Yang et al, 2003;Liang et al, 2012), has been reported to improve plant resistance to herbicide napropamide toxicity in rapeseed (Brassica napus) (Cui et al, 2010).…”

The Effects of Salicylic Acid on Helianthus annuus L. Exposed to Quizalofop-P-Ethyl

“…It was reported that the pre-treatment of plants with SA before the application of herbicides, such as paraquat, caused protection against paraquat-induced damage because of changes in the antioxidant enzyme activities [77]. SA ameliorated napropamide-induced oxidative stress in rapeseed Brassica napus [78]. The modification of the antioxidant status of plant cells by SA application was previously investigated with pathogens [79] [80].…”

Long-term response on growth, antioxidant enzymes, and secondary metabolites in salicylic acid pre-treated Uncaria tomentosa microplants

“…DMSO (Table 3). In plants of Brassica napus plants exposed to the herbicide, napropamide, and treated with 100 lM SA, had decreased activities of SOD, CAT and ascorbate peroxidase while POX and glutathione-S-transferase activities increased (Cui et al 2010).…”