Sagittula marina sp. nov., isolated from seawater and emended description of the genus Sagittula

Abstract

A novel bacterium, designated strain F028-2 T , was isolated from seawater at Damupo beach in Pohang, Korea, and investigated in a taxonomic study using a polyphasic approach. This novel strain was strictly aerobic, non-motile, Gram-stain-negative and rod-shaped, and occasionally formed aggregates. The temperature, pH and NaCl ranges for growth were 4-30 6C, pH 6.5-9.0 and 1-7 % (w/v), respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain F028-2 T formed a lineage within the family Rhodobacteraceae of the class Alphaproteobacteria, and was closely related to members of the genera Sagittula and Antarctobacter with 96.3-96.4 % sequence similarities. The polar lipid profile of strain F028-2 T comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, one unidentified phospholipid and six unidentified lipids. The predominant cellular fatty acids were C 18 : 1 v7c and C 12 : 1 3-OH. The genomic DNA G+C content of strain F028-2 T was 61.6 mol% and the major respiratory quinone was Q-10. Based on phenotypic, phylogenetic and genotypic data, strain F028-2 T is considered to represent a novel species in the genus Sagittula, for which the name Sagittula marina sp. nov. is proposed. The type strain is F028-2 T (5KCTC 23543 T 5JCM 17627 T ). An emended description of the genus Sagittula is also proposed.The family Rhodobacteraceae belonging to the order Rhodobacterales, class Alphaproteobacteria, phylum Proteobacteria, is one of the most abundant bacterial groups in marine environments. Within the family Rhodobacteraceae, the Roseobacter clade is known to be one of the most abundant groups, typically comprising upwards of 20 % of coastal and 15 % of mixed-layer ocean bacterioplankton communities (González & Moran, 1997;Buchan et al., 2005). Strains in the Roseobacter clade have been isolated from various marine habitats such as seawater, sediment, seaweed, a tidal flat, marine electroactive biofilms, the Arctic Ocean and the phycosphere of a dinoflagellate (Lafay et al., 1995;Yoon et al., 2007;Zhang et al., 2008;Vandecandelaere et al., 2008 Vandecandelaere et al., , 2009. They play important roles in the carbon, sulfur and nitrogen cycles in the ocean (González et al., 1999(González et al., , 2000.In the course of investigations of bacterial diversity in seawater, a large number of novel bacterial strains were isolated. In this study, one such strain, F028-2 T , which was affiliated with the Roseobacter clade within the Rhodobacteraceae, was subjected to further taxonomic investigation. Strain F028-2 T was isolated from a seawater sample collected at Damupo beach in Pohang, Korea (36 u 029 510 N 129 u 359 100 E), by using a standard serial dilution plating method on marine agar 2216 (MA; Difco). The strain was initially grown at 25 u C for 7 days and then routinely maintained on MA, with subculture every 3 days, under aerobic conditions at 25 u C. The strain was also stored at 280 u C in marine broth 2216 (MB; Dif...

Sagittula salina sp. nov., isolated from marine waste

“…Strain M10.9X T was able to synthesize phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The polar lipid profile of strain M10.9X T is in accordance with the data previously reported by Lee et al [2] for S. marina F028-2 T , which was also able to synthesize phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid and six unidentified lipids.…”

“…The genus Sagittula was first described by González et al in 1997 and reclassified within the Rhodobacteraceae family by Lee et al in 2012 [1,2]. At the time of writing, the genus Sagittula is composed of only two species: Sagittula stellata and Sagittula marina, both isolated from marine environments and promising strains with bioremediation capacities [1][2][3].…”

Genotypic Variation of Endophytic Nitrogen-Fixing Activity and Bacterial Flora in Rice Stem Based on Sugar Content

“…Bootstrap values of ≥50% are indicated at their respective nodes. Reference type strains are indicated with superscript “†.” The backgrounds of the reference strains are colored according to the sugar (sucrose and/or glucose) utilization capacity of those bacteria based on the data from (1) Lee et al ( 2013 ); (2) Staley ( 1984 ); (3) Kuykendall et al ( 2015 ); (4) Dall'Agnol et al ( 2013 ); (5) Baldani et al ( 2015 ); (6) Hungria et al ( 1993 ); (7) Kuykendall ( 2015 ); (8) Sajnaga and Jach ( 2020 ); (9) Ahnia et al ( 2018 ); (10) Reis et al ( 2004 ); (11) Chen et al ( 2006 ); (12) Coenye et al ( 2001 ); (13) Wolterink et al ( 2005 ); (14) Weon et al ( 2008 ); (15) Qiu et al ( 2017 ); (16) Hardoim et al ( 2013 ); (17) Xu et al ( 2020 ); (18) Ueki et al ( 2006 ); (19) Olsen ( 2015 ); (20) Imhoff ( 2015b ); (21) Diaz-Barrera et al ( 2016 ); (22) Bae et al ( 2006 ); (23) Norris et al ( 2015 ); and (24) Patrick and McDowell ( 2015 ). The black-and-white shading in the columns on the right-hand of the NifH-OTU name indicates the mean relative abundance of the NifH-OTUs in each rice genotype ( n = 2 for Nipponbare; n = 3 for agpl1 , Leafstar, and CG14).…”

Frigidibacter albus gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from lake water

“…Members of the family Rhodobacteraceae are physiologically and metabolically heterogeneous, including both phototrophic and non-phototrophic bacteria. In recent years, many novel species isolated from various habitats have been shown to belong taxonomically to the family Rhodobacteraceae , such as species of the genera Youngimonas (Hameed et al , 2014), Cribrihabitans (Chen et al , 2014), Planktomarina (Giebel et al , 2013), Pelagimonas (Hahnke et al , 2013), Profundibacterium (Lai et al , 2013), Sagittula (Lee et al , 2013a), Epibacterium (Penesyan et al , 2013) and Falsirhodobacter (Subhash et al , 2013). In this communication, we report a novel non-phototrophic species of a new genus associated with the family Rhodobacteraceae , which was isolated from a cold-water lake.…”