Safety evaluation of the plasma on ocular surface tissue: An animal study and histopathological findings

Nejat, Farhad; Nabavi, Nazanin-Sadat; Nejat, Mohammad-Amin; Aghamollaei, Hossein; Jadidi, Khosrow

doi:10.1016/j.cpme.2019.100084

Cited by 19 publications

(24 citation statements)

References 23 publications

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“…Based on the above-mentioned bits of knowledge, we hypothesized that the plasma technology can be used for the treatment of some ocular surface disorders. Then, as our first step, we conducted an animal study on the safety evaluation of plasma that showed no serious complications for plasma on the conjunctival tissue after one-month intervention [ 13 ]. In the present study, for achieving more reliable results, the histopathological changes of the conjunctiva were examined after 6 months of exposure to plasma.…”

Section: Discussionmentioning

confidence: 99%

“…Considering the potential of the Plexr in medical applications, determination of the safety profile of ALTP for various applications, including the ocular structure, seemed necessary. Accordingly, Nejat et.al (2019) assessed the effects of Plexr on the ocular surface in animal models [ 13 ]. They evaluated the histopathological changes on the conjunctiva after 1 month of plasma exposure.…”

Section: Introductionmentioning

confidence: 99%

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Safety evaluation of the atmospheric low-temperature plasma (ALTP) on the conjunctiva: an animal study and histopathological findings; 6-month follow-up

et al. 2021

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Background Plasma medicine is an innovative research field focused on the application of atmospheric-pressure low-temperature plasmas (ALTP) for therapeutic purposes. Considering the potentials of plasma in ophthalmology, in this study, we evaluated the safety of plasma on the conjunctival tissue in animal models for 6 months. Methods Twelve adult male New Zealand albino rabbits were divided into four groups. The right eye of each rabbit was chosen for the test and the left eye was considered as the control. Experiments were performed using the Plexr device (GMV, Rocca Priora, RM, Italy). Four plasma spots were applied on the superior part of the conjunctiva (from 10 to 2 o’clock positions) using the continuous mode and a low power level (white handpiece) of the Plexr. For evaluation of the plasma safety, the histopathological changes were assessed 1 week (A), 1 month (B), 3 months (C), and 6 months (D) after the intervention. Results According to the histopathological findings, a mild decrease in blood vessels and severe stromal edema, as well as a superficial epithelium loss, were observed in group A. No chronic inflammation, scar tissue, deposition, and hemorrhage were found in group B. Epithelialization was confirmed by the histological examinations after 1 month. There was no evidence of atypia or dysplasia after 3 and 6 months. Conclusion In conclusion, there were no persistent histopathological changes on conjunctival tissue after plasma exposure. Then, plasma can be considered as a minimally invasive alternative method for treating some ocular surface disorders.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Safety evaluation of the atmospheric low-temperature plasma (ALTP) on the conjunctiva: an animal study and histopathological findings; 6-month follow-up

et al. 2021

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“…This finding confirms no damage to the deep tissue layers. 14 Fifth, the Plexr device operates at temperatures slightly higher than room temperature (30-60 °C) and by applying it, the electric current does not pass through the tissue. However, in order to cut, coagulate and fuse the tissue, the electrocautery uses very high temperature and the electrosurgery passes an electrical current through the tissue.…”

Section: 455mentioning

confidence: 99%

“…So, it seems there is the potential to use this type of plasma to treat some ocular surface disorders. 14 In the following study, we introduce a new and simple plasma-based approach to conjunctival cyst removal.…”

Section: Introductionmentioning

confidence: 99%

<p>A Novel Approach to Treatment of Conjunctival Cyst Ablation Using Atmospheric Low-Temperature Plasma</p>

Nejat

Jadidi

Pirhadi

et al. 2020

OPTH

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Introducing a new technique to remove the conjunctival cyst using atmospheric low-temperature plasma (ALTP) and assessing its effectiveness. Patients and Methods: Five eyes with conjunctival cysts were included in this study. The procedure was started by applying a plasma spot on the highest point of the cyst, and then other spots were spirally applied to the base of the cyst to debulk it. The conjunctival cysts were removed using the white handpiece of the plasma generator device (Plexr, GMV s.r.l Grottaferrata, Italy). Refraction, visual acuity, intraocular pressure (IOP), contrast sensitivity (CS), aberrometry, dry eye tests, and ocular surface disease index (OSDI) questionnaire were measured before, one and six months after treatment. Results: The mean size of the cysts was 3.6 mm (range 2.1 to 4.8 mm). No intraoperative and postoperative complications were observed. Postoperative examinations showed that the patients' cysts were completely healed and recurrence did not occur. Significant changes in visual parameters, IOP, CS, aberrometry and dry eye tests were not observed in the follow-up periods after treatment compared to before. OSDI score decreased after cyst removal. Conclusion: Our recommended technique using ALTP can be used as a new approach to treating conjunctival cysts. This technique is a simple, office-based, minimally invasive, effective and inexpensive way to remove a cyst.

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“…Plasma-assisted noninvasive surgery (PANIS) has been developed and used in the ophthalmic field by Nejat et al for the first time. In this therapeutic method, the atmospheric pressure low-temperature plasma (ALTP) [35] is used to treat ocular surface disorders such as conjunctival cyst [36] and conjunctivochalasis [37] and doubtless they had already assessed the safety of this method in animal models using histopathological examinations and cytokine factors assessments [38]. In the present study, the clinical efficacy of this inexpensive and simple method has been evaluated, for temporary punctal occlusion in order to treat DED.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of efficacy of plasma assisted noninvasive surgery (PANIS) as a novel approach for temporary punctal occlusion: a clinical case series

Nejat¹,

Jadidi²,

Eghtedari³

et al. 2021

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Safety evaluation of the plasma on ocular surface tissue: An animal study and histopathological findings

Cited by 19 publications

References 23 publications

Safety evaluation of the atmospheric low-temperature plasma (ALTP) on the conjunctiva: an animal study and histopathological findings; 6-month follow-up

Safety evaluation of the atmospheric low-temperature plasma (ALTP) on the conjunctiva: an animal study and histopathological findings; 6-month follow-up

<p>A Novel Approach to Treatment of Conjunctival Cyst Ablation Using Atmospheric Low-Temperature Plasma</p>

Evaluation of efficacy of plasma assisted noninvasive surgery (PANIS) as a novel approach for temporary punctal occlusion: a clinical case series

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