Safety assessment of Syzygium aromaticum flower bud (clove) extract with respect to testicular function in mice

Mishra, Raghav Kumar; Singh, Shio Kumar

doi:10.1016/j.fct.2008.08.006

Cited by 65 publications

(48 citation statements)

References 22 publications

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“…The reduction in testicular weight might be attributable to a direct effect of the extract on the histoarchitecture of the testis (Figure 4) as reported by other investigators (Bone et al, 2000) leading to depletion of germinal or spermatogenic elements and ultimately to disruption of spermatogenesis. The seminiferous tubules make up about 90% of the wet weight of the testis (Mishra and Singh, 2008). The reduction could also be due to a reduction in serum testosterone concentration (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Antispermatogenic effects of aqueous ethanolic extract of Hymenocardia acida stem bark in Wistar rats

Abu¹,

Uchendu²

2010

J. Med. Plants Res.

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Oral administration of aqueous ethanolic extract of Hymenocardia acida stem bark for eight weeks caused a significant reduction (P < 0.05) in the weights of testes, epididymides, ventral prostrate, seminal vesicles and vasa differentia compared to the control. The treatment related decreases in sperm motility, count, viability and serum testosterone returned to near normal levels on withdrawal of the extract. The groups that received doses of 100, 200 and 400 mg/kg body weight of the extract showed 40, 0 and 0% fertility, respectively. H. acida stem bark extract adversely affected spermatogenesis in Wistar rats.

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Section: Discussionmentioning

confidence: 99%

Antispermatogenic effects of aqueous ethanolic extract of Hymenocardia acida stem bark in Wistar rats

Abu¹,

Uchendu²

2010

J. Med. Plants Res.

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“…Syzygium aromaticum, a medicinal plant commonly known as clove, is native to tropical America and Australia (Mishra and Singh 2008) and has many medicinal properties. It is used to treat toothache, respiratory disorders, inflammation, and gastrointestinal disorders, and as an antibacterial (Domaracky et al 2007;Gurib-Fakim 2006;Kim et al 1998Kim et al , 2003Ghelardini et al 2001;Muruganandan et al 2001;Cruz 1979;Moreira 1978).…”

Section: Introductionmentioning

confidence: 99%

Gastroprotective activity of essential oil of the Syzygium aromaticum and its major component eugenol in different animal models

Santin

Lemos

Klein‐Júnior

et al. 2010

Naunyn-Schmied Arch Pharmacol

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Syzygium aromaticum, a medicinal plant commonly known as clove, is used to treat toothache, respiratory disorders, inflammation, and gastrointestinal disorders. From the flower buds of S. aromaticum, it is possible to obtain an essential oil comprised of a mixture of aliphatic and cyclic volatile terpenes and phenylpropanoids, being eugenol as the main component. The aims of this study were: (1) to extract the essential oil of the flower buds of S. aromaticum, (2) to identify and quantify the main component of the essential oil, and (3) to evaluate its antiulcer activity using different animal models. Assays were performed using the following protocols in rats: indomethacin-induced and ethanol/HCl-induced ulcer model. Both essential oils from S. aromaticum and eugenol displayed antiulcer activities in the rat models of indomethacin- and ethanol-induced ulcer. Studies focusing on the possible mechanisms of gastroprotection were also undertaken using the following experiments: evaluation of gastric secretion by the pylorus-ligated model, determination of mucus in gastric content, participation of nitric oxide (NO) and endogenous sulfhydryl in gastric protection. The results show that there was no significant effect on the volume of gastric juice and total acidity. However, the quantification of free gastric mucus showed that the clove oil and eugenol were capable of significantly enhancing mucus production. With regard to the NO and endogenous sulfhydryls, the results demonstrated that the gastroprotection induced by clove oil and eugenol are not related to the activities of the nitric oxide and endogenous sulfhydryls. No sign of toxicity was observed in the acute toxicity study. In conclusion, the results of this study show that essential oil of S. aromaticum, as well as its main component (eugenol), possesses antiulcer activity. The data suggest that the effectiveness of the essential oil and eugenol is based on its ability to stimulate the synthesis of mucus, an important gastroprotective factor. However, further pharmacological and toxicological investigations are required to enable its use for the treatment of gastric ulcer.

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“…Thirty-two (age: 14-15 weeks, weight 32-34 g) male laboratory mice of Parkes (P) strain from closed and randomly bred colony[9] were used in this study. Mice were randomly allocated into four groups (eight animals per group), with mice of same body weights in a group.…”

Section: Methodsmentioning

confidence: 99%

Reproductive effects of lipid soluble components of Syzygium aromaticum flower bud in male mice

Mishra

Singh

2013

J Ayurveda Integr Med

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Background:The flower buds of Syzygium aromaticum (clove) have been used in indigenous medicines for the treatment of male sexual disorders in Indian subcontinent.Objective:To evaluate the effect of Syzygium aromaticum flower bud on male reproduction, using Parkes (P) strain mice as animal model.Materials and Methods:Mice were orally administered lipid soluble components of Syzygium aromaticum flower bud in doses of 15, 30, and 60 mg/kg body weight for 35 days, and several male reproductive endpoints were evaluated.Results:Treatment with lower dose (15 mg) of Syzygium increased the motility of sperm and stimulated the secretory activities of epididymis and seminal vesicle, while higher doses (30 and 60 mg) had adverse effects on sperm dynamics of cauda epididymidis and on the secretory activities of epididymis and seminal vesicle. Libido was not affected in treated males; however, a significant decrease in litter in females sired by males treated with higher doses of Syzygium was recorded.Conclusion:Treatment with Syzygium aromaticum flower bud causes dose-dependent biphasic effect on male reproductive indices in P mice; lower dose of Syzygium appears stimulatory, while the higher doses have adverse effect on male reproduction. The results suggest that the lower dose of Syzygium may have androgenic effect, but further studies are needed to support this contention.

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Safety assessment of Syzygium aromaticum flower bud (clove) extract with respect to testicular function in mice

Cited by 65 publications

References 22 publications

Antispermatogenic effects of aqueous ethanolic extract of Hymenocardia acida stem bark in Wistar rats

Antispermatogenic effects of aqueous ethanolic extract of Hymenocardia acida stem bark in Wistar rats

Gastroprotective activity of essential oil of the Syzygium aromaticum and its major component eugenol in different animal models

Reproductive effects of lipid soluble components of Syzygium aromaticum flower bud in male mice

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