Safe use of Cry genes in genetically modified crops

Rahman, Mehboob‐ur; Zaman, Muhammad Arif Uz; Shaheen, Tayyaba; Irem, Samra; Zafar, Yusuf

doi:10.1007/s10311-015-0508-4

Cited by 29 publications

(23 citation statements)

References 65 publications

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“…The lack of significant biological differences in leukon values across dietary treatments in the present study suggested that transgenic cottonseed of up to 40% could safely be incorporated in the rabbit's diet without having any detrimental effect on their health. The result of this study is in agreement with that of Rahman et al () who reported that the total leucocytes count and differential leucocytes count did not differ when rabbits were fed a diet containing up to 30% of Bt cottonseed for 90 days. Similarly (Yang et al, ), reported non‐significant differences in the leucocytes values when transgenic poplar leaves harbouring binary insect resistant gene (chitinase‐ BmkIT) were fed to rabbits for 70 days.…”

Section: Discussionsupporting

confidence: 93%

Effect of dietary supplementation of recombinant Cry and Cp4 epsps proteins on haematological indices of growing rabbits

Salisu

Shahid

Yaqoob

et al. 2018

Animal Physiology Nutrition

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Genetically modified (GM) crops expressing insect resistance and herbicide tolerance provide a novel approach for improved crop production but their advent at the same time presents serious challenges in terms of food safety. Although prevailing scientific proof has suggested that transgenic crops are analogous to their conventional counterparts, their use in human and animal diet gave rise to emotional public discussion. A number of studies had been conducted to evaluate the potential unintended effects of transgenic crops expressing single transgene, but very few studies for those with multiple transgenes. As the crops with single and multiple transgenes could impart different effects on non‐target organisms, thus, risk evaluation of transgenic crops expressing more than one transgene is required to declare their biosafety. The present study was therefore designed to assess the effects of different levels of dietary transgenic cottonseed expressing recombinants proteins produced by Cry1Ac, Cry2A and Cp4epsps genes on haematological indices of growing rabbits. A total of 48 rabbits were assigned to four dietary treatments containing different levels of transgenic cottonseeds (i.e., 0% w/w, 20% w/w, 30% w/w and 40% w/w) with 0% w/w serving as control. Haematological parameters were measured at periodic intervals (0, 45, 90, 135 and 180) days. No significant (p > 0.05) dose‐dependent effects were observed in most of the haematological parameters evaluated. Though, significant differences (p < 0.05) were recorded in the level of MCHC, MCH and HCT in some of experimental male and female rabbits, yet, they were not biologically significant, as all the differences were within the normal reference values. Our study suggested that feeding transgenic cottonseed of up to 40% could not adversely affect rabbit's haematological profile. However, further study needs to be conducted with different cotton genotypes expressing both single and polygenic traits before recommending the utilization of transgenic cottonseed in routine livestock feeding.

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Section: Discussionsupporting

confidence: 93%

Effect of dietary supplementation of recombinant Cry and Cp4 epsps proteins on haematological indices of growing rabbits

Salisu

Shahid

Yaqoob

et al. 2018

Animal Physiology Nutrition

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“…The excessive accumulation of exogenous proteins during inappropriate developmental periods or in undesired tissues might have unexpected consequences, including delayed development and abnormal morphology (Ma et al, 2009;Xu et al, 2006). Besides, Bt protein expressed in edible endosperm might arouse food safety questions regarding Bt insecticidal proteins in transgenic rice, although there is no scientific basis or credible evidence supporting such concerns (Rahman et al, 2015). It is therefore desirable to engineer plants that express the foreign toxin gene and accumulate transgenic protein only in target tissues.…”

Section: Introductionmentioning

confidence: 99%

Isolation of five rice nonendosperm tissue‐expressed promoters and evaluation of their activities in transgenic rice

et al. 2017

Plant Biotechnology Journal

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SummaryUsing promoters expressed in nonendosperm tissues to activate target genes in specific plant tissues or organs with very limited expression in the endosperm is an attractive approach in crop transgenic engineering. In this article, five putative nonendosperm tissue‐expressed promoters were cloned from the rice genome and designated PO s NETE 1, PO s NETE 2, PO s NETE 3, PO s NETE 4 and PO s NETE 5. By qualitatively and quantitatively examining GUSplus reporter gene expression in transgenic rice plants, PO s NETE 1‐PO s NETE 5 were all found to be active in the roots, leaves, stems, sheaths and panicles but not in the endosperm of plants at different developmental stages. In addition, PO s NETE 2, PO s NETE 4 and PO s NETE 5 were also inactive in rice embryos. Among these promoters, PO s NETE 4 and PO s NETE 5 exhibited higher activities in all of the tested tissues, and their activities in stems, leaves, roots and sheaths were higher than or comparable to those of the rice Actin1 promoter. We also progressively monitored the activities of PO s NETE 1‐PO s NETE 5 in two generations of single‐copy lines and found that these promoters were stably expressed between generations. Transgenic rice was produced using PO s NETE 4 and PO s NETE 5 to drive a modified Bt gene, mC ry1Ab. Bt protein expressed in the tested plants ranged from 1769.4 to 4428.8 ng/g fresh leaves, whereas Bt protein was barely detected in the endosperm. Overall, our study identified five novel nonendosperm tissue‐expressed promoters that might be suitable for rice genetic engineering and might reduce potential social concern regarding the safety of GMO crops.

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“…Genomic technologies have been used to improve cultivated crop species, which provides an opportunity to transfer new specific traits of interest into other valuable genotypes (GMO-genetically modified organism) within a short period of time, and greatly reduces costs by promoting crop yield and environmental risk by decreasing the use of chemical insecticides (Peferoen, 1997;Baute et al, 2002;Bourguet et al, 2002;Rahman et al, 2015). On a global basis in 2010, about 15.4 million farmers grew biotech crops on about 1 billion ha (James, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…It is crucial that risk assessment studies on the commercial use of Bt crops consider the impacts on organisms in soil. Generally, few or no toxic effects of Cry proteins on woodlice, collembolans, mites, earthworms, nematodes, protozoa, and the activity of various enzymes in soil have been addressed (Icoz and Stotzky, 2008;Rahman et al, 2015). Although some effects, ranging from no effect to minor and significant effects, of Bt plants on microbial communities in soil have been reported, they were mostly the result of differences in geography, temperature, plant variety, and soil type and, in general, were transient and not related to the presence of the Cry proteins (Icoz and Stotzky, 2008).…”

Section: Introductionmentioning

confidence: 99%

Soil Ecological Safety Evaluation for Bivalent Transgenic Cotton Plants: Root Exudates Versus Soil Enzyme Activities and Soil Microbial Diversity

Wu¹

2016

Appl Ecol Env Res

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Abstract. Pot experiments were conducted to assess the possibly adverse effect of transgenic Bt-and CpTI-cotton (Bacillus thuringiensis and cowpea trypsin inhibitor cotton) on soil ecosystem. Soil enzymes activities and microbial molecular community and diversity based on 16s-and 18s-PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles were determined by adding root exudates of Bt-and CpTI-cotton into soil. Results showed that the root exudates of transgenic cotton seedlings increased the soil catalase activity by 21.8% to 32.7%. The soil urease activity was decreased by 11.0% while the activity in the treatment of 1ml/350g soil was increased by 18.1% compared to control. The invertase activity in the treatment of 1 ml/350g soil was increased by 54.2%, however, the activity in the treatments of 2 and 4 ml/350 g soil was decreased by from 25% to 29.2%. The total 16sPCR-DGGE lane bands of bacterial community and diversity were almost not different. However, the effect pattern of root exudates of transgenic cotton on soil fungal community and diversity was differed from bacteria. The fungal community number and composition were changed. Though some dominant community disappeared and other newly dominant community appeared, the fungal stability index was increased. Results suggested there was little significantly adverse effect of Bt-and CpTI-cotton on soil ecosystem.

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Safe use of Cry genes in genetically modified crops

Abstract: Genomic technologies have been used to improve cultivated crop species.

Cited by 29 publications

References 65 publications

Effect of dietary supplementation of recombinant Cry and Cp4 epsps proteins on haematological indices of growing rabbits

Effect of dietary supplementation of recombinant Cry and Cp4 epsps proteins on haematological indices of growing rabbits

Isolation of five rice nonendosperm tissue‐expressed promoters and evaluation of their activities in transgenic rice

Soil Ecological Safety Evaluation for Bivalent Transgenic Cotton Plants: Root Exudates Versus Soil Enzyme Activities and Soil Microbial Diversity

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