Sacrificial mold-assisted 3D printing of stable biocompatible gelatin scaffolds

Nagarajan, S.; Belaid, Habib; Radhakrishnan, Socrates; Teyssier, Catherine; Balme, Sébastien; Miele, Philippe; Cornu, David; Subbaraya, Narayana Kalkura; Cavaillès, Vincent; Bechelany, Mikhaël

doi:10.1016/j.bprint.2021.e00140

Cited by 20 publications

(12 citation statements)

References 33 publications

(37 reference statements)

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“…However, additive manufacturing (3D printing) can be used to produce an intricate negative mold in a dissolvable thermoplastic material such as polyvinyl alcohol (PVA). Researchers have used 3D printed PVA molds in applications such as microfluidic channels 10 , biocompatible gelatin scaffolds 11 , ceramic injection molding of parts 12 , and rocket engine components 13 .…”

Section: Figure 1 Neuron Components Of Pyramidal Cellmentioning

confidence: 99%

3D Printing of Flexible, Scaled Neuron Models

Habbal

Farhat

Khalil

et al. 2023

Preprint

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Microscopy-based 3D neuronal reconstructions are freely available online, including in the NeuroMorpho.Org file repository. Each neuron’s dendritic structure is intricate and diverse, making it challenging to produce accurate physical 3D models for instruction or visualization. This work examines several methods for producing 3D models of neuronal reconstructions and compares their cost and accessibility. In response to high cost of direct 3D printing methods, we develop a new casting method which uses 3D-printed, single-use dissolvable molds and achieves lower cost for producing 3D neuron models. The casting method uses a consumer-grade desktop fused filament fabrication 3d printer, water-soluble polyvinyl alcohol filament, and a two-part casting material such as polyurethane resin or silicone rubber. Physical models of a diverse set of neuron morphologies including purkinje, pyramidal, medium spiny, and retinal ganglion cells were produced using the casting method with good fidelity to the neuronal reconstruction file and sufficient detail and strength for hands-on use in neuroscience education and research. The average cost of producing the four neuron models using the proposed casting method was reduced by 58% relative to the cost of using the least expensive 3D printing method by a service provider. Production time for one neuronal model using the proposed method was found to be in the range of 1-3 days while service-provided neurons required a minimum of a week from order placement to delivery.

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Section: Figure 1 Neuron Components Of Pyramidal Cellmentioning

confidence: 99%

3D Printing of Flexible, Scaled Neuron Models

Habbal

Farhat

Khalil

et al. 2023

Preprint

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“…HUVECs were later injected into the perfusible channel and attached to the surface of the branched channel, proving that the remaining materials were nontoxic to cells. In 2021, Nagarajan et al filled gelatin solution into a 3D printed PVA scaffold [ 70 ]. After the gelatin solution was cross-linked with glutaraldehyde, the construct was soaked in hot water to remove the sacrificial PVA.…”

Section: Sacrificial Biomaterials Based On Physical Principlesmentioning

confidence: 99%

Application Status of Sacrificial Biomaterials in 3D Bioprinting

Liu

Wang

et al. 2022

Polymers

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Additive manufacturing, also known as three-dimensional (3D) printing, relates to several rapid prototyping (RP) technologies, and has shown great potential in the manufacture of organoids and even complex bioartificial organs. A major challenge for 3D bioprinting complex org unit ans is the competitive requirements with respect to structural biomimeticability, material integrability, and functional manufacturability. Over the past several years, 3D bioprinting based on sacrificial templates has shown its unique advantages in building hierarchical vascular networks in complex organs. Sacrificial biomaterials as supporting structures have been used widely in the construction of tubular tissues. The advent of suspension printing has enabled the precise printing of some soft biomaterials (e.g., collagen and fibrinogen), which were previously considered unprintable singly with cells. In addition, the introduction of sacrificial biomaterials can improve the porosity of biomaterials, making the printed structures more favorable for cell proliferation, migration and connection. In this review, we mainly consider the latest developments and applications of 3D bioprinting based on the strategy of sacrificial biomaterials, discuss the basic principles of sacrificial templates, and look forward to the broad prospects of this approach for complex organ engineering or manufacturing.

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“…Recently, increasing attention has been paid to the research of polymer-based BTES, especially through the combination of natural polymers and synthetic polymer materials to create new tissue engineering scaffolds [ 13 , 14 , 15 , 16 ]. Bone tissue is composed of water, organic matter, and inorganic salt.…”

Section: Introductionmentioning

confidence: 99%

Unraveling of Advances in 3D-Printed Polymer-Based Bone Scaffolds

et al. 2022

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The repair of large-area irregular bone defects is one of the complex problems in orthopedic clinical treatment. The bone repair scaffolds currently studied include electrospun membrane, hydrogel, bone cement, 3D printed bone tissue scaffolds, etc., among which 3D printed polymer-based scaffolds Bone scaffolds are the most promising for clinical applications. This is because 3D printing is modeled based on the im-aging results of actual bone defects so that the printed scaffolds can perfectly fit the bone defect, and the printed components can be adjusted to promote Osteogenesis. This review introduces a variety of 3D printing technologies and bone healing processes, reviews previous studies on the characteristics of commonly used natural or synthetic polymers, and clinical applications of 3D printed bone tissue scaffolds, analyzes and elaborates the characteristics of ideal bone tissue scaffolds, from t he progress of 3D printing bone tissue scaffolds were summarized in many aspects. The challenges and potential prospects in this direction were discussed.

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Sacrificial mold-assisted 3D printing of stable biocompatible gelatin scaffolds

Cited by 20 publications

References 33 publications

3D Printing of Flexible, Scaled Neuron Models

3D Printing of Flexible, Scaled Neuron Models

Application Status of Sacrificial Biomaterials in 3D Bioprinting

Unraveling of Advances in 3D-Printed Polymer-Based Bone Scaffolds

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