Saccharothrix isguenensis sp. nov., an actinobacterium isolated from desert soil

Abstract

A novel actinobacterial strain, designated MB27T, was isolated from a Saharan soil sample collected in Mzab region (Ghardaïa province, South Algeria). Strain MB27T was characterized following a polyphasic taxonomic approach. This strain produced a branched and fragmented substrate mycelium, which was found to have a yellowish orange colour. A white scanty aerial mycelium was produced on most media tested. Chemotaxonomic and phylogenetic studies clearly demonstrated that strain MB27T belongs to the family Pseudonocardiaceae and is closely related to the genus Saccharothrix. Cell-wall hydrolysates contained meso-diaminopimelic acid but not glycine, and whole-cell hydrolysates contained galactose, glucose, ribose and small amounts of mannose and rhamnose. The detected phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Mycolic acids were not detected while the predominant fatty acid was iso-branched hexadecanoate (iso-C16 : 0). The major menaquinone was MK-9(H4). Results of 16S rRNA gene sequence comparisons revealed that strain MB27T shairs the highest degree of similarity with Saccharothrix ecbatanensis DSM 45486T (99.8%), Saccharothrix hoggarensis DSM 45457T (99.3 %), Saccharothrix longispora DSM 43749T (98.6 %) and Saccharothrix yanglingensis DSM 45665T (98.6 %). However, it exhibited only 11-42 % DNA-DNA relatedness to the neighbouring Saccharothrixspecies. On the basis of phenotypic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridization, strain MB27T is shown to represent a novel species of the genus Saccharothrix, for which the name Saccharothrix isguenensis sp. nov. (type strain MB27T=DSM 46885T=CECT 9045T) is proposed.

Solihabitans fulvus gen. nov., sp. nov., a member of the family Pseudonocardiaceae isolated from soil

“…One siderophore, one melanin, one lanthipeptide, Table 1. Phenotypic characteristics of strain AN110305 T and related species of the family Pseudonocardiaceae Taxa: 1, strain AN110305 T ; 2, Goodfellowiella [21,22]; 3, Umezawaea [53,54]; 4, Kutzneria [55][56][57][58]; 5, Saccharothrix [59][60][61][62][63][64]. Data were from this study unless indicated.…”

Antimicrobial activities of novel bipyridine compounds produced by a new strain of Saccharothrix isolated from Saharan soil

“…The arid soils of the Algerian Sahara have been shown to be rich in members of the Saccharothrix genus (Sabaou et al, 1998, Zitouni et al, 2005) including new species, such as Saccharothrix algeriensis NRRL B-24137 T (Zitouni et al, 2004a), Saccharothrix hoggarensis DSM 45457 T , Saccharothrix saharensis DSM 45456 T , Saccharothrix tamanrassetensis DSM 45947 T (Boubetra et al, 2013a, Boubetra et al, 2013b, Boubetra et al, 2015), Saccharothrix isguenensis DSM 46885 T and Saccharotrix ghardaiensis DSM 46886 T (Bouznada et al, 2016, Bouznada et al, 2017). In addition, many studies have shown the ability of Saccharothrix strains isolated from these soils to produce new or known antibiotics, such as dithiolopyrrolones (Lamari et al, 2002, Bouras et al, 2008, Merrouche et al, 2011), anthracyclines (Zitouni et al, 2004b) and chloramphenicol (Aouiche at al., 2012).…”