Antimicrobial activities of novel bipyridine compounds produced by a new strain of Saccharothrix isolated from Saharan soil

Lahoum, Abdelhadi; Sabaou, Nasserdine; Bijani, Christian; Bouras, Noureddine; Pont, Frédéric; Snini, Selma P.; Mathieu, Florence

doi:10.1016/j.jsps.2018.07.019

Cited by 16 publications

(13 citation statements)

References 27 publications

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“…Blast analysis based on the 16S rRNA gene sequence showed strain 16Sb2-4 shared the highest nucleotide sequence similarity (99.7%) with Saccharothrix xinjiangensis NBRC 101911 T , a strain that was previously isolated from Tianchi Lake, Xinjiang Uygur Autonomous Region, China ( Hu et al, 2004 ). Strain of S. xinjiangensis has ever been reported to produce a range of bioactive compounds, such as the tianchimycins A and B ( Wang et al, 2013 ), cyanogriside I and J, caerulomycin A and F ( Lahoum et al, 2019 ), and caerulomycin M and saccharopyrone ( Babadi et al, 2020 ). Taking these facts into consideration, strain 16Sb2-4 was prioritized to conduct a chemical analysis to gain deeper insights into its bioactive metabolites.…”

Section: Resultsmentioning

confidence: 99%

“…More than 20 macrolides were isolate from different Saccharothrix sp., including cytotoxic 10/11-membered macrolides saccharothriolides A–K, X ( Lu et al, 2015 , 2016 ) and 20-membered macrolides ammocidins A–D ( Murakami et al, 2001 , 2009 ); antibacterial 16-membered macrolides Tianchimycins A and B ( Wang et al, 2013 ), aldgamycins G, H, and swalpamycin B ( Mizobuchi et al, 1986 ; Zitouni et al, 2007 ; Wang et al, 2013 ); and antifungal 16-membered macrolides formamicin ( Igarashi et al, 1997 ). Recently, new antibacterial congeners cyanogriside J and M were isolated from strain ABH26, which was closely related to S. xinjiangensis NBRC 101911 T ( Lahoum et al, 2019 ); and new cytotoxic compounds, caerulomycin M, saccharopyrone and saccharonoic acid were isolated from S. xinjiangensis Act24Zk ( Skellam et al, 2013 ). These promising results emphasize the need to continue the research in the genus Saccharothrix .…”

Section: Discussionmentioning

confidence: 99%

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Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Liu

Wang

et al. 2021

Front. Microbiol.

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Taklamakan desert is known as the largest dunefield in China and as the second largest shifting sand desert in the world. Although with long history and glorious culture, the Taklamakan desert remains largely unexplored and numerous microorganisms have not been harvested in culture or taxonomically identified yet. The main objective of this study is to explore the diversity, novelty, and pharmacological potential of the cultivable actinomycetes from soil samples at various sites along the Alar-Hotan desert highway in the Taklamakan desert. A total of 590 actinobacterial strains were recovered by the culture-dependent approach. Phylogenetic analysis based on 16S ribosomal RNA (rRNA) gene sequences unveiled a significant level of actinobacterial diversity with 55 genera distributed in 27 families of 12 orders. Thirty-six strains showed relatively low 16S rRNA similarities (<98.65%) with validly described species, among which four strains had already been characterized as novel taxa by our previous research. One hundred and forty-six actinobacterial isolates were selected as representatives to evaluate the antibacterial activities and mechanism of action by the paper-disk diffusion method and a double fluorescent protein reporter “pDualrep2” system, respectively. A total of 61 isolates exhibited antagonistic activity against the tested “ESKAPE” pathogens, among which seven strains could produce bioactive metabolites either to be able to block translation machinery or to induce SOS-response in the pDualrep2 system. Notably, Saccharothrix sp. 16Sb2-4, harboring a promising antibacterial potential with the mechanism of interfering with protein translation, was analyzed in detail to gain deeper insights into its bioactive metabolites. Through ultra-performance liquid chromatography (UPLC)-quadrupole time-of-flight (QToF)-MS/MS based molecular networking analysis and databases identification, four families of compounds (1–16) were putatively identified. Subsequent bioassay-guided separation resulted in purification of four 16-membered macrolide antibiotics, aldgamycin H (8), aldgamycin K (9), aldgamycin G (10), and swalpamycin B (11), and their structures were elucidated by HR-electrospray ionization source (ESI)-MS and NMR spectroscopy. All compounds 8–11 displayed antibacterial activities by inhibiting protein synthesis in the pDualrep2 system. In conclusion, this work demonstrates that Taklamakan desert is a potentially unique reservoir of versatile actinobacteria, which can be a promising source for discovery of novel species and diverse bioactive compounds.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Liu

Wang

et al. 2021

Front. Microbiol.

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“…Very recently, a novel Saccharothrix xinjiangensis ABH26 strain, isolated from a soil sample collected in the Adrar region (Southern Algeria), was studied by Lahoum et al [107]. The new metabolites, named cyanogriside I (44) and J (45), were purified, and the suggested structures were indicated as a methoxy-bipyridine linked to sugar units through an O-oxime moiety.…”

Section: Antimicrobialsmentioning

confidence: 99%

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

et al. 2019

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Actinobacteria, in particular “rare actinobacteria” isolated from extreme ecosystems, remain the most inexhaustible source of novel antimicrobials, offering a chance to discover new bioactive metabolites. This is the first overview on actinobacteria isolated in Algeria since 2002 to date with the aim to present their potential in producing bioactive secondary metabolites. Twenty-nine new species and one novel genus have been isolated, mainly from the Saharan soil and palm groves, where 37.93% of the most abundant genera belong to Saccharothrix and Actinopolyspora. Several of these strains were found to produce antibiotics and antifungal metabolites, including 17 new molecules among the 50 structures reported, and some of these antibacterial metabolites have shown interesting antitumor activities. A series of approaches used to enhance the production of bioactive compounds is also presented as the manipulation of culture media by both classical methods and modeling designs through statistical strategies and the associations with diverse organisms and strains. Focusing on the Algerian natural sources of antimicrobial metabolites, this work is a representative example of the potential of a closely combined study on biology and chemistry of natural products.

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“…As a result, from 2002 to 2019, one new genus and twenty-nine new species were obtained, mainly from palm groves and Algerian Saharan soils. Several of these strains were proved capable of producing antibacterial and antifungal metabolites, including 17 new molecules among the 50 structures reported (Djinni et al 2019;Lahoum et al 2019). Actinobacteria isolated from arid and other extreme ecosystems remain the inexhaustible source of new antimicrobials, offering an opportunity to get new bioactive metabolites (Djinni et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

2020

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Abstract. Aouar L, Boukelloul I, Benadjila A. 2020. Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties. Biodiversitas 21: 5672-5683. To produce new bioactive substances of agricultural interest, extreme ecosystems can be a source of unexplored microorganisms. Accordingly, in this study, twenty-two actinobacteria strains were obtained from rhizospheric arid soils of palm groves collected from Biskra and El Oued in the Algerian Sahara. All isolates were examined for the in vitro antifungal potential towards phytopathogenic fungi: Aspergillus flavus, Verticillium dahlia, Rhizoctonia solani, Botrytis cinerea and Fusarium oxysporum as well as for their antibacterial property toward phytopathogenic bacteria: Streptomyces scabiei, Pectobacterium carotovorum and Agrobacterium tumefaciens. The three isolates (13%) that inhibited at least five pathogens were then selected, identified and assessed for their attributes to produce indole-3-acetic acid (IAA) and siderophores, to solubilize phosphate, and to antagonize Streptomyces scabiei in vivo. According to phylogenetic analysis performed with 16S rDNA sequence, chemotaxonomy and phenotypic characteristics, the strain SO1, which inhibited all tested pathogens, was assigned to Streptomyces flaveus. While, strains SO2 and SB1 were affiliated to Streptomyces enissocaesilis and Streptomyces albidoflavus, respectively. All strains produced IAA but only SO1 and SB1 were able to elaborate siderophores catecholate-type. Two strains SO1 and SO2 exhibited a capacity to solubilize phosphate and SO1 was able to suppress the pathogenic effect of Streptomyces scabiei on radish seedlings. The findings indicate that SO1 strain may reveal the potential for use as a biocontrol agent and plant growth promoter.

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Antimicrobial activities of novel bipyridine compounds produced by a new strain of Saccharothrix isolated from Saharan soil

Cited by 16 publications

References 27 publications

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

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