Saccharothrix algeriensis sp. nov., isolated from Saharan soil

Abstract

The taxonomic position of a soil isolate, strain SA 233T, recovered from Saharan soil from Algeria was established using a polyphasic approach. This isolate has been previously reported to produce three novel dithiolopyrrolone antibiotics, and preliminary chemotaxonomic and morphological characteristics suggested that it was representative of a member of the genus Saccharothrix. Phylogenetic analysis of the strain from 16S rDNA sequences, along with a detailed analysis of morphological, chemotaxonomic and physiological characteristics, indicates that it belongs to the genus Saccharothrix and represents a novel species that is readily distinguished from all recognized Saccharothrix species. The name Saccharothrix algeriensis sp. nov. is proposed for the isolate, with type strain SA 233T (=NRRL B-24137T=DSM 44581T).

Saccharothrix sp. PAL54, a new chloramphenicol-producing strain isolated from a Saharan soil

“…DNA-DNA relatedness between strains was determined as described previously (Zitouni et al 2004a) in 59 SSC (19 SSC is 0.15 M sodium chloride and 0.015 M sodium citrate) and 20% dimethyl sulfoxide at 66°C (melting point -23°C) by the method of De Ley et al (1970), using a Perkin Elmer Lambda 35UV/VIS spectrophotometer fitted with the Peltier temperature controller, PTP-1. The experiments were performed in duplicate.…”

Saccharothrix saharensis sp. nov., an actinomycete isolated from Algerian Saharan soil

“…In recent years, several Saccharothrix strains have been transferred to other new taxa, including the genera Lentzea (Yassin et al, 1995), Crossiella (Labeda, 2001), Lechevalieria (Labeda et al, 2001), Goodfellowia (Labeda & Kroppenstedt, 2006) and Umezawaea (Labeda & Kroppenstedt, 2007). At the time of writing, the genus Saccharothrix includes 13 recognized species.In the course of isolation of Saharan actinomycetes, several novel strains and species of Saccharothrix have been isolated from Algerian Saharan soil, such as Saccharothrix algeriensis DSM 44581T (Zitouni et al, 2004a) and Saccharothrix hoggarensis DSM 45457 T (Boubetra et al, 2013). Furthermore, many of these strains were shown to produce interesting antibiotics such as mutactimycins (Zitouni et al, 2004b), dithiolopyrrolones (Bouras et al, 2008) and chloramphenicol (Aouiche et al, 2012).…”

Isolation, Taxonomy, and Antagonistic Properties of Halophilic Actinomycetes in Saharan Soils of Algeria

“…Genomic DNA of 30 strains, belonging to different clusters established by numerical taxonomy, was extracted for 16S rRNA gene analysis according to the method of Liu et al (16). The 16S rRNA gene was PCR amplified by the method cited by Zitouni et al (34). Phylogenetic analysis was performed as described by Boudjelal et al (4).…”