Saccharomyces cerevisiae KNU5377 Stress Response during High-Temperature Ethanol Fermentation

Kim, Il-Sup; Kim, Young-Saeng; Kim, Hyun; Jin, Ingnyol; Yoon, Ho-Sung

doi:10.1007/s10059-013-2258-0

Cited by 24 publications

(12 citation statements)

References 40 publications

(51 reference statements)

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“…Consistent with our findings, the thermotolerant S. cerevisiae KNU5377 strain has been shown to be tolerant to ethanol, and oxidative stresses (Kim et al 2013). In addition, several natural isolates of S. cerevisiae have been reported to be resistant to multiple stresses.…”

Section: Discussionsupporting

confidence: 92%

“…It is possible that the ability to minimize the intracellular ROS levels in these multiple stress-tolerant strains may be due to high ROS-scavenging activities and/or effective stress responses. In agreement with this idea, it has been shown that, during fermentation, the thermotolerant KNU5377 strain exhibited a high expression of Yap1p, a major transcription factor involved in activating the transcription of antioxidant genes in response to oxidative stress (Kim et al 2013), suggesting its high ability to cope with oxidative stress and to maintain redox balance. Previously, it has been shown that an increase of β-1,6-glucan levels was associated with a reduced sensitivity of yeast cells to Zymolyase, suggesting that the degree of β-1,6-glucosidic cross-linking between β-1,3-glucan, mannoprotein and chitin may contribute to cell wall robustness (Aguilar-Uscanga and Francois 2003).…”

Section: Discussionmentioning

confidence: 62%

“…Based on these previous findings, it is therefore possible that cellular mechanisms responsible for thermotolerance may also play an important role in protecting yeast cells against other environmental stresses. Consistent with this idea, the thermotolerant S. cerevisiae KNU5377 strain has been reported to be also tolerant to ethanol, and oxidative stresses (Kim et al 2013). …”

Section: Introductionmentioning

confidence: 68%

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Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation

et al. 2016

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High-temperature ethanol fermentation has several benefits including a reduction in cooling cost, minimizing risk of bacterial contamination, and enabling simultaneous saccharification and fermentation. To achieve the efficient ethanol fermentation at high temperature, yeast strain that tolerates to not only high temperature but also the other stresses present during fermentation, e.g., ethanol, osmotic, and oxidative stresses, is indispensable. The C3253, C3751, and C4377 Saccharomyces cerevisiae strains, which have been previously isolated as thermotolerant yeasts, were found to be multiple stress-tolerant. In these strains, continuous expression of heat shock protein genes and intracellular trehalose accumulation were induced in response to stresses causing protein denaturation. Compared to the control strains, these multiple stress-tolerant strains displayed low intracellular reactive oxygen species levels and effective cell wall remodeling upon exposures to almost all stresses tested. In response to simultaneous multi-stress mimicking fermentation stress, cell wall remodeling and redox homeostasis seem to be the primary mechanisms required for protection against cell damage. Moreover, these strains showed better performances of ethanol production than the control strains at both optimal and high temperatures, suggesting their potential use in high-temperature ethanol fermentation.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 68%

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Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation

et al. 2016

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“…In response to heat, S. cerevisiae typically shows transcriptional changes in genes encoding metabolic enzymes (e.g., hexokinase, glyceraldehyde-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, and ADH), antioxidant enzymes (e.g., thioredoxin 3, thioredoxin reductase, and porin), molecular chaperones and their cofactors (e.g., HSP104 , HSP82 , HSP60 , HSP42 , HSP30 , HSP26 , CPR1 , STI1 , and ZPR1 ), and the TFs (e.g., HSF1 , MSN2/4 , and YAP1 ), among others (Lindquist, 1986 ; Piper, 1993 ; Kim et al, 2013 ). Most of these genes also change their expression in response to ethanol and high osmolarity (Gasch et al, 2000 ; Gasch and Werner-Washburne, 2002 ).…”

Section: The Cellular Stress Response In Yeastmentioning

confidence: 99%

Modifying Yeast Tolerance to Inhibitory Conditions of Ethanol Production Processes

Caspeta

Castillo

Nielsen

2015

Front. Bioeng. Biotechnol.

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Saccharomyces cerevisiae strains having a broad range of substrate utilization, rapid substrate consumption, and conversion to ethanol, as well as good tolerance to inhibitory conditions are ideal for cost-competitive ethanol production from lignocellulose. A major drawback to directly design S. cerevisiae tolerance to inhibitory conditions of lignocellulosic ethanol production processes is the lack of knowledge about basic aspects of its cellular signaling network in response to stress. Here, we highlight the inhibitory conditions found in ethanol production processes, the targeted cellular functions, the key contributions of integrated -omics analysis to reveal cellular stress responses according to these inhibitors, and current status on design-based engineering of tolerant and efficient S. cerevisiae strains for ethanol production from lignocellulose.

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“…In nature, yeast cells are in constant flux between respiration and fermentation depending on sugar and oxygen levels [ 51 ]. These constant changes in the cell’s metabolic state require a highly responsive and dynamic control of gene expression [ 52 , 53 ]. In high concentration of glucose, yeast cells prefer fermentative metabolism to oxidative pathway regardless of oxygen levels [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Wide Annotation of Eukaryotic RNase III Reactivity and Degradation Signals

et al. 2015

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Detection and validation of the RNA degradation signals controlling transcriptome stability are essential steps for understanding how cells regulate gene expression. Here we present complete genomic and biochemical annotations of the signals required for RNA degradation by the dsRNA specific ribonuclease III (Rnt1p) and examine its impact on transcriptome expression. Rnt1p cleavage signals are randomly distributed in the yeast genome, and encompass a wide variety of sequences, indicating that transcriptome stability is not determined by the recurrence of a fixed cleavage motif. Instead, RNA reactivity is defined by the sequence and structural context in which the cleavage sites are located. Reactive signals are often associated with transiently expressed genes, and their impact on RNA expression is linked to growth conditions. Together, the data suggest that Rnt1p reactivity is triggered by malleable RNA degradation signals that permit dynamic response to changes in growth conditions.

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Saccharomyces cerevisiae KNU5377 Stress Response during High-Temperature Ethanol Fermentation

Cited by 24 publications

References 40 publications

Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation

Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation

Modifying Yeast Tolerance to Inhibitory Conditions of Ethanol Production Processes

Transcriptome Wide Annotation of Eukaryotic RNase III Reactivity and Degradation Signals

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