S100B-mediated protection against the pro-apoptotic effects of ethanol on fetal rhombencephalic neurons

Druse, Mary J.; Gillespie, Roberta A.; Tajuddin, Nuzhath F.; Rich, Megan

doi:10.1016/j.brainres.2007.02.092

Cited by 29 publications

(27 citation statements)

References 61 publications

(102 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…While their neuroprotective effects are undoubtedly due in part to their actions as classical antioxidants, the present studies raise the possibility that these effects might also involve the up-regulation of genes that encode pro-survival/anti-apoptotic proteins. Interestingly, this laboratory demonstrated that two additional neuroprotective agents, i.e., the 5-HT 1A agonist ipsapirone and S100B, both prevented ethanol-associated apoptosis and augmented expression of XIAP and either Bcl- xl or Bcl-2 (Druse et al, 2006; 2007). A common thread in the neuroprotective effects of three unrelated types of agents, i.e., antioxidants, ipsapirone, and S100B, might involve their ability to up-regulate the expression of these and possibly other neuroprotective genes.…”

Section: Discussionmentioning

confidence: 99%

“…Ethanol also reduces serotonin (5-HT) neurons and their projections Tajuddin and Druse, 1999; 2001; Sari and Zhou, 2004; Zhou et al, 2005). The ethanol-associated loss of neurons appears to be caused by apoptotic cell death (Ramachandran et al, 2003; Druse et al, 2004; 2005; 2007; Dikranian et al, 2005; Chen et al, 2006). Apoptotic death of neurons is preceded by increased reactive oxygen species (ROS) and mitochondrial dysfunction (Chu et al, 2007) as well as damage to DNA (Cherian et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antioxidant neuroprotection against ethanol-induced apoptosis in HN2-5 cells

2009

Self Cite

View full text Add to dashboard Cite

Earlier studies from this and other laboratories show that ethanol induces apoptotic death of fetal and neonatal neurons. One mechanism that underlies these effects is the ethanol-associated reduction in the phosphatidylinositol 3′ kinase pro-survival pathway. Another mechanism involves the oxidative stress caused by the ethanol-associated increase in reactive oxygen species (ROS).In the present study, we used the murine HN2-5 hippocampal-derived cell line to investigate the effects of ethanol on ROS levels and apoptosis. We also investigated the potential neuroprotective effects of two structurally unrelated antioxidants: N-acetylcysteine (NAC) and melatonin. The results demonstrate that NAC blocked an ethanol-associated increase in ROS. In addition, NAC and melatonin prevented the augmentation of apoptosis in ethanol-treated neurons. Both antioxidants significantly elevated the expression of the anti-apoptotic gene XIAP in ethanol-treated and/or control neurons and melatonin increased Bcl-2 expression in ethanol-treated neurons. Thus, it is possible that the neuroprotective effects of NAC and melatonin involve their ability to augment the expression of one or more anti-apoptotic gene as well as their classical antioxidant actions. Additional studies are needed to establish the effectiveness of these antioxidants to prevent the loss of neurons which accompanies in utero exposure to ethanol.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidant neuroprotection against ethanol-induced apoptosis in HN2-5 cells

2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, TCP1alpha levels are known to positively affect the expression of several structural related genes involved with chromatin remodeling and microtubulin assembly (Giuffrida et al 2006;Schuller et al 2001). TCP1alpha and S100B both are involved in regulating cellular apoptosis (Chan et al 2006;Druse et al 2007).…”

Section: Averaged Expressionmentioning

confidence: 99%

Microarray analysis of differential utilization of plant-based diets by rainbow trout

et al. 2011

View full text Add to dashboard Cite

Microarray analysis was conducted using liver samples from two families of rainbow trout that differed in their growth responses when compared between individuals fed a fishmeal or plant protein-based diet. Differential expression relating to dietary utilization between the two families found significant changes in expression of 33 expressed sequence tags (ESTs). Eight of the differentially expressed ESTs had identified mammalian homologs that had been previously researched with identified cellular interactions and functions. Utilizing pathway analysis software to analyze sequences annotated with known mammalian genes, we were able to map gene pathways and process interactions. From this information, we were able to infer that the metabolic changes associated with utilization of plant protein versus fishmeal were associated with differential regulation of genes related to cell oxidative stress, proliferation, growth and survival. Furthermore, we inferred from the changes we observed in immune response gene expression that ingestion of this plantbased diet upregulated the expression of genes involved in immunoregulatory processes.

show abstract

“…Druse et al (2007) described the association between phosphoinositide 3-kinase and serotonin on fetal rhombencephalic neurons in rat fetuses treated with alcohol. Takahashi et al (2005) provided a detailed review of the critical role of the phosphoinositide 3-kinase pathway in the proliferation, survival, and maintenance of pluripotency in embryo stem cells.…”

Section: Representation Of Genes and Gene Familiesmentioning

confidence: 99%

Advancing the understanding of the embryo transcriptome co-regulation using meta-, functional, and gene network analysis tools

Rodriguez-Zas¹,

Ko²,

Adams³

et al. 2008

Reproduction

View full text Add to dashboard Cite

Embryo development is a complex process orchestrated by hundreds of genes and influenced by multiple environmental factors. We demonstrate the application of simple and effective meta-study and gene network analyses strategies to characterize the co-regulation of the embryo transcriptome in a systems biology framework. A meta-analysis of nine microarray experiments aimed at characterizing the effect of agents potentially harmful to mouse embryos improved the ability to accurately characterize gene co-expression patterns compared with traditional within-study approaches. Simple overlap of significant gene lists may result in under-identification of genes differentially expressed. Sample-level meta-analysis techniques are recommended when common treatment levels or samples are present in more than one study. Otherwise, study-level meta-analysis of standardized estimates provided information on the significance and direction of the differential expression. Cell communication pathways were highly represented among the genes differentially expressed across studies. Mixture and dependence Bayesian network approaches were able to reconstruct embryo-specific interactions among genes in the adherens junction, axon guidance, and actin cytoskeleton pathways. Gene networks inferred by both approaches were mostly consistent with minor differences due to the complementary nature of the methodologies. The top-down approach used to characterize gene networks can offer insights into the mechanisms by which the conditions studied influence gene expression. Our work illustrates that further examination of gene expression information from microarray studies including meta-and gene network analyses can help characterize transcript co-regulation and identify biomarkers for the reproductive and embryonic processes under a wide range of conditions. Reproduction (2008) 135 213-224

show abstract

S100B-mediated protection against the pro-apoptotic effects of ethanol on fetal rhombencephalic neurons

Cited by 29 publications

References 61 publications

Antioxidant neuroprotection against ethanol-induced apoptosis in HN2-5 cells

Antioxidant neuroprotection against ethanol-induced apoptosis in HN2-5 cells

Microarray analysis of differential utilization of plant-based diets by rainbow trout

Advancing the understanding of the embryo transcriptome co-regulation using meta-, functional, and gene network analysis tools

Contact Info

Product

Resources

About