S100+ cells: A new neuro-immune cross-talkers in lymph organs

Huang, Jinyu; Zhu, Chunfang; Zhang, Peipei; Zhu, Qing; Liu, Yanmei; Zhu, Zhansheng; Wang, Min-Chen; Li, Wenjie; Yang, Gang; Dong, Nan; Liu, Juan; Chen, Lian; Zhang, Yanlin; Yang, Run-Lin; Deng, Lili; Fan, Jun; Wang, Xiaochun; Li, Jun; Ma, Bin; Fu, Qiang; Wu, Kaiyun

doi:10.1038/srep01114

Cited by 16 publications

(27 citation statements)

References 17 publications

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“…4 (c) and (d)), which additionally confirms the stability of the model drug loading under cell culture conditions. 29 This lack in the uptake of free model drug at dye concentration levels present in the supernatant after release indicates that a cellular uptake of drugs is favored by intracellular release of drugs from loaded particles from the film surface under the studied conditions, fully in line with previously reported results obtained for spin-coated particulate films. 16 The possibility of particle-mediated transport of drugs was investigated by further cell experiments.…”

Section: Drug Loading and Releasesupporting

confidence: 90%

Cell Adherence and Drug Delivery from Particle Based Mesoporous Silica Films

Björk

Baumann²,

Hausladen³

et al. 2019

Preprint

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Spatially and temporally controlled drug delivery is important for implant and tissue engineering applications, as the efficacy and bioavailability of the drug can be enhanced, and can also allow for drugging stem cells at different stages of development. Long-term drug delivery over weeks to months is however difficult to achieve, and coating of 3D surfaces or creating patterned surfaces is a challenge using coating techniques like spin- and dip-coating. In this study, mesoporous films consisting of SBA-15 particles grown onto silicon wafers using wet processing were evaluated as a scaffold for drug delivery. Films with various particle sizes (100 – 900 nm) and hence thicknesses were grown onto OTS-functionalized silicon wafers using a direct growth method. Precise patterning of the areas for film growth could be obtained by local removal of the OTS functionalization through laser ablation. The films were incubated with the model drug DiO, and murine myoblast cells (C2C12 cells) were seeded onto films with different particle sizes. Confocal laser scanning microscopy (CLSM) was used to study the cell growth, and a vinculin-mediated adherence of C2C12 cells on all films was verified. The successful loading of DiO into the films was confirmed by UV-vis and CLSM. It was observed that the drugs did not desorb from the particles during 24 hours in cell culture. During adherent growth on the films for 4 h, small amounts of DiO and separate particles were observed inside single cells. After 24 h, a larger number of particles and a strong DiO signal were recorded in the cells, indicating a particle mediated drug uptake. A substantial amount of DiO loaded particles were however attached on the substrate after 24 making the films attractive as a long-term reservoir for drugs on e.g. medical implants.<br>

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Section: Drug Loading and Releasesupporting

confidence: 90%

Cell Adherence and Drug Delivery from Particle Based Mesoporous Silica Films

Björk

Baumann²,

Hausladen³

et al. 2019

Preprint

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“…Compared with another study of the sympathetic innervation of rat cervical lymph nodes by using anterograde tracking of nerves with Fluoro-Ruby (a neuronal tracer) 34 revealing the varicosities (nerve endings) of sympathetic fibers only, our study has not only demonstrated the distribution of NMSC/Remak fibers, but also revealed another kind of NMSC-immune cell contact. Compared with another innervation study of the lymph node by means of an anti-NF antibody, 14 we have also observed more NMSCs including cell bodies and processes inside the B cell follicle; this might also indicate the additional presence of more nerve fibers in the cortex region including B cell follicles and their Germinal centers.…”

Section: Discussionmentioning

confidence: 75%

“… 1-2 ,6, 10 These tight microanatomical connections between the cells of the two systems provide the structural support of the complex network of immune responses. Until now, despite some reported studies, 11 , 13 , 14 , 34 , 35 detailed microanatomical descriptions of innervation of lymph nodes have been limited. By using anti-GFAP as a reliable cellular marker, we have analyzed the distribution of NMSCs in the mesenteric lymph nodes.…”

Section: Discussionmentioning

confidence: 99%

Immunofluorescent characterization of non-myelinating Schwann cells and their interactions with immune cells in mouse mesenteric lymph node

et al. 2017

Self Cite

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The central nervous system (CNS) influences the immune system in a general fashion by regulating the systemic concentration of humoral substances, whereas the autonomic nervous system communicates specifically with the immune system according to local interactions. Data concerning the mechanisms of this bidirectional crosstalk of the peripheral nervous system (PNS) and immune system remain limited. To gain a better understanding of local interactions of the PNS and immune system, we have used immunofluorescent staining of glial fibrillary acidic protein (GFAP), coupled with confocal microscopy, to investigate the non-myelinating Schwann cell (NMSC)-immune cell interactions in mouse mesenteric lymph nodes. Our results demonstrate: i) the presence of extensive NMSC processes and even of cell bodies in each compartment of the mouse mesenteric lymph node; ii) close associations/interactions of NMSC processes with blood vessels (including high endothelial venules) and the lymphatic vessel/sinus; iii) close contacts/associations of NMSC processes with various subsets of dendritic cells (such as CD4+CD11c+, CD8+CD11c+ dendritic cells), macrophages (F4/80+ and CD11b+ macrophages), and lymphocytes. Our novel findings concerning the distribution of NMSCs and NMSC-immune cell interactions inside the mouse lymph node should help to elucidate the mechanisms through which the PNS affects cellular- and humoral-mediated immune responses or vice versa in health and disease.

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“…4(c) and (d)), which additionally conrms the stability of the model drug loading under cell culture conditions. 34 The lack of free model drug in the supernatant indicates that the drug must be delivered to the cells according to a different mechanism. Previous studies have reported that a cellular uptake of hydrophobic drugs is favored by intracellular release of drugs from loaded particles taken up from the lm surface.…”

Section: Model Drug Loading and Releasementioning

confidence: 99%

Cell adherence and drug delivery from particle based mesoporous silica films

Björk

Baumann

Hausladen³

et al. 2019

RSC Adv.

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Spatially and temporally controlled drug delivery is important for implant and tissue engineering applications, as the efficacy and bioavailability of the drug can be enhanced, and can also allow for drugging stem cells at different stages of development. Long-term drug delivery over weeks to months is however difficult to achieve, and coating of 3D surfaces or creating patterned surfaces is a challenge using coating techniques like spin-and dip-coating. In this study, mesoporous films consisting of SBA-15 particles grown onto silicon wafers using wet processing were evaluated as a scaffold for drug delivery.Films with various particle sizes (100-900 nm) and hence thicknesses were grown onto trichloro(octadecyl)silane-functionalized silicon wafers using a direct growth method. Precise patterning of the areas for film growth could be obtained by local removal of the OTS functionalization through laser ablation. The films were incubated with the drug model 3,3 0 -dioctadecyloxacarbocyanine perchlorate (DiO), and murine myoblast cells (C2C12 cells) were seeded onto films with different particle sizes. Confocal laser scanning microscopy (CLSM) was used to study the cell growth, and a vinculinmediated adherence of C2C12 cells on all films was verified. The successful loading of DiO into the films was confirmed by UV-vis and CLSM. It was observed that the drugs did not desorb from the particles during 24 hours in cell culture. During adherent growth on the films for 4 h, small amounts of DiO and separate particles were observed inside single cells. After 24 h, a larger number of particles and a strong DiO signal were recorded in the cells, indicating a particle mediated drug uptake. The vast majority of the DiO-loaded particles remained attached to the substrate also after 24 h of incubation, making the films attractive as longer-term reservoirs for drugs on e.g. medical implants. † Electronic supplementary information (ESI) available: Details on substrate preparation, TEM micrographs of the materials, a CLSM micrograph of a lm aer 24 h of cell cultivation, and a CLSM micrograph of C2C12 cells on a lm. See

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S100+ cells: A new neuro-immune cross-talkers in lymph organs

Cited by 16 publications

References 17 publications

Cell Adherence and Drug Delivery from Particle Based Mesoporous Silica Films

Cell Adherence and Drug Delivery from Particle Based Mesoporous Silica Films

Immunofluorescent characterization of non-myelinating Schwann cells and their interactions with immune cells in mouse mesenteric lymph node

Cell adherence and drug delivery from particle based mesoporous silica films

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