S‐phase fraction and DNA ploidy in oral leukoplakia

Khanna, Rahul; Agarwal, Anshu; Khanna, Seema; Basu, Somprakas; Khanna, Ajay K.

doi:10.1111/j.1445-2197.2009.05196.x

Cited by 16 publications

(25 citation statements)

References 10 publications

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“…This suggestion, however, though in agreement with previous reports specifically addressing DNA aneuploidy as a biomarker of this risk [15,23], is not yet proven. On the other hand, it is important to point out that the predictive value of both dysplasia and anatomical subsite remains in dispute [24-28].…”

Section: Discussionsupporting

confidence: 66%

Chromosomal aberrations and aneuploidy in oral potentially malignant lesions: distinctive features for tongue

Castagnola¹,

Malacarne²,

Scaruffi

et al. 2011

BMC Cancer

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BackgroundThe mucosae of the oral cavity are different at the histological level but appear all equally exposed to common genotoxic agents. As a result of this exposure, changes in the mucosal epithelia may develop giving rise to Oral Potentially Malignant Lesions (OPMLs), which with time may in turn progress to Oral Squamous Cell Carcinomas (OSCCs). Therefore, much effort should be devoted to identify features able to predict the likeliness of progression associated with an OPML. Such features may be helpful in assisting the clinician to establish both appropriate therapies and follow-up schedules. Here, we report a pilot study that compared the occurrence of DNA aneuploidy and chromosomal copy number aberrations (CNAs) in the OPMLs from different oral anatomical subsites.MethodsSamples from histologically diagnosed OPMLs were processed for high resolution DNA flow cytometry (hr DNA-FCM) in order to determine the relative DNA content expressed by the DNA index (DI). Additionally, array-Comparative Genomic Hybridization (a-CGH) analysis was performed on DNA obtained from diploid nuclei suspensions directly. When aneuploid nuclei were detected, these were physically separated from diploid nuclei on the base of their DI values by means of a DNA-FCM-Sorter in order to improve the a-CGH analysis.ResultsTongue OPMLs were more frequently associated with DNA aneuploidy and CNAs than OPMLs arising from all the other mucosal subsites.ConclusionsWe suggest that the follow-up and the management of the patients with tongue OPMLs should receive a distinctive special attention. Clearly, this hypothesis should be validated in a prospective clinical study.

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Section: Discussionsupporting

confidence: 66%

Chromosomal aberrations and aneuploidy in oral potentially malignant lesions: distinctive features for tongue

Castagnola¹,

Malacarne²,

Scaruffi

et al. 2011

BMC Cancer

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“…Amplification of cyclin E and CDK2 genes play a role in colorectal tumorigenicity [20]. To estimate the rate of cell proliferation, the SPF was frequently used in breast cancer, thyroid cancer and oral leukoplakia [21-23]. In colon cancer, high SPF was significantly associated with a poor prognosis [24,25].…”

Section: Discussionmentioning

confidence: 99%

Up-regulation of CNDP2 facilitates the proliferation of colon cancer

Xue

Zhang

et al. 2014

BMC Gastroenterol

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BackgroundCytosolic nonspecific dipetidase (CN2) belongs to the family of M20 metallopeptidases. It was stated in previous articles that higher expression levels of CN2 were observed in renal cell carcinoma and breast cancer. Our study explored the correlation between CN2 and colon carcinogenesis.MethodsWe analysed the relationship between 183 patients clinicopathological characteristics and its CN2 expression. To detect the levels of CN2 in colon cancer cell lines and colon cancer tissues by western blot. To verify cell proliferation in colon cancer cells with knockdown of CNDP2 and explore the causes of these phenomena.ResultsThe expression levels of CN2 in clinical colon tumors and colon cancer cell lines were significantly higher than that in normal colon mucosa and colon cell lines. The difference in CN2 levels was associated with tumor location (right- and left-sided colon cancer), but there was no significant association with age, gender, tumor size, tumor grade, tumor stage or serum carcinoembryonic antigen (CEA). Knockdown of CNDP2 inhibited cell proliferation, blocked cell cycle progression and retarded carcinogenesis in an animal model. The signaling pathway through which knockdown of CNDP2 inhibited cell proliferation and tumorigenesis involved in EGFR, cyclin B1 and cyclin E.ConclusionsKnockdown of CNDP2 can inhibit the proliferation of colon cancer in vitro and retarded carcinogenesis in vivo.

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“…Concrescence should be carefully identified to reduce the risk of complications associated with surgical procedures [31]. It may affect the extraction of an adjacent tooth and may fracture the tuberosity or f loor of the maxillary sinus.…”

Section: Concrescencementioning

confidence: 99%

“…It is suspected that space restriction during development, local trauma, excessive occlusal force, or local infection after development play an important role in the occurrence of concrescence [18,31]. True concrescence is attributed to the close proximity of developing roots of the adjacent teeth whereas acquired concrescence may result from a chronic inflammatory response to a non-vital tooth [42].…”

Section: Concrescencementioning

confidence: 99%

Developmental anomalies affecting the morphology of teeth – a review

Shrestha

Marla

Shrestha

et al. 2016

RSBO

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Introduction: The development of tooth is a complex process wherein there is series of interactions between the ectoderm and ectomesenchyme. The role of genes in determining the shape and form of a specific tooth has already been defined, the alterations in which can lead to a variety of anomalies in regards to number, size, form, shape, structure, etc. Objective: To review the literature on the developmental anomalies of teeth. Literature review: The developmental anomalies affecting the morphology exists in both deciduous & permanent dentition and shows various forms such as gemination, fusion, concrescence, dilacerations, dens evaginatus, dens invaginatus, enamel pearls, taurodontism or peg laterals. These anomalies have clinical significance concerning esthetics, malocclusion and more importantly predisposing the development of dental caries and periodontal diseases. Conclusion: Knowledge of various diagnostic criteria for identification of these developmental anomalies is significant for early diagnosis and pertinent treatment.

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S‐phase fraction and DNA ploidy in oral leukoplakia

Abstract: The method of flow cytometry can be used to identify the subset of leukoplakia patients who are at a higher risk of malignant conversion. These patients could undergo more rigid surveillance or undergo excision biopsy of their lesions.

Cited by 16 publications

References 10 publications

Chromosomal aberrations and aneuploidy in oral potentially malignant lesions: distinctive features for tongue

Chromosomal aberrations and aneuploidy in oral potentially malignant lesions: distinctive features for tongue

Up-regulation of CNDP2 facilitates the proliferation of colon cancer

Developmental anomalies affecting the morphology of teeth – a review

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