S-phase fraction, 5-bromo-2′-deoxy-uridine labelling index, duration of S-phase, potential doubling time, and DNA index in benign and malignant brain tumors

Struikmans, H.; Rutgers, Derk H.; Jansen, Gerard H.; Tulleken, C. A. F.; Tweel, Ingeborg van der; Battermann, Jan J.

doi:10.1002/(sici)1520-6823(1997)5:4<170::aid-roi2>3.0.co;2-v

Cited by 20 publications

(4 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The anti-BrdU antibody specifically detects bromodeoxyuridine (BrdU), a haplopyrimidine that labels proliferating cells in S phase (Struikmans et al, 1997). This was confirmed by testing the antibody’s precipitation against 5-Methyl Cysteine (5-MeC), BrdU or no antigen (as a control).…”

Section: Methodsmentioning

confidence: 99%

Nuclear factor one B regulates neural stem cell differentiation and axonal projection of corticofugal neurons

Betancourt

Katzman

Chen

2013

J of Comparative Neurology

View full text Add to dashboard Cite

During development of the cerebral cortex, neural stem cells divide to expand the progenitor pool and generate basal progenitors, outer radial glia and cortical neurons. As these newly born neurons differentiate, they must properly migrate toward their final destination in the cortical plate, project axons to appropriate targets, and develop dendrites. However, a complete understanding of the precise genetic mechanisms regulating these steps is lacking. Here we show that a member of the nuclear factor one (NFI) family of transcription factors, NFIB, is essential for many of these processes in mice. We performed a detailed analysis of NFIB expression during cortical development, and investigated defects in cortical neurogenesis, neuronal migration and differentiation in NfiB−/− brains. We found that NFIB is strongly expressed in radial glia and corticofugal neurons throughout cortical development. However, in NfiB−/− cortices, radial glia failed to generate outer radial glia, subsequently resulting in a loss of late basal progenitors. In addition, corticofugal neurons showed a severe loss of axonal projections, while late-born cortical neurons displayed defects in migration and ectopically expressed the early-born neuronal marker, CTIP2. Furthermore, gene expression analysis, by RNA-sequencing, revealed a misexpression of genes that regulate the cell cycle, neuronal differentiation and migration in NfiB−/− brains. Together these results demonstrate the critical functions of NFIB in regulating cortical development.

show abstract

Section: Methodsmentioning

confidence: 99%

Nuclear factor one B regulates neural stem cell differentiation and axonal projection of corticofugal neurons

Betancourt

Katzman

Chen

2013

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…The brominated thymidine analog 5‐bromo‐2′‐deoxyuridine (BrdU) incorporates into DNA during the S phase of the cell cycle and can therefore be used to detect DNA synthetic events, including cell division, DNA repair, and cell cycle reentry [1, 2–3]. BrdU is commonly used to quantitate the proliferative index [4, 5] or to birthdate, identify, and track cycling endogenous and transplanted central nervous system (CNS) stem/progenitor cells [6, 7, 8, 9, 10, 11–12]. However, quantitative comparison of independent studies of neurogenesis is difficult because BrdU does not exclusively label dividing cells, and there is no standardized dosing schedule (see [13] for review).…”

Section: Introductionmentioning

confidence: 99%

Bromodeoxyuridine Induces Senescence in Neural Stem and Progenitor Cells

et al. 2008

View full text Add to dashboard Cite

Bromodeoxyuridine (BrdU) is a halogenated pyrimidine that incorporates into newly synthesized DNA during the S phase. BrdU is used ubiquitously in cell birthdating studies and as a means of measuring the proliferative index of various cell populations. In the absence of secondary stressors, BrdU is thought to incorporate relatively benignly into replicating DNA chains. However, we report here that a single, low-dose pulse of BrdU exerts a profound and sustained antiproliferative effect in cultured murine stem and progenitor cells. This is accompanied by altered terminal differentiation, cell morphology, and protein expression consistent with the induction of senescence. There is no evidence of a significant increase in spontaneous cell death; however, cells are rendered resistant to chemically induced apoptosis. Finally, we show that a brief in vivo BrdU regimen reduces the proliferative potential of subsequently isolated subependymal zone neurosphere-forming cells. We conclude, therefore, that BrdU treatment induces a senescence pathway that causes a progressive decline in the replication of rapidly dividing stem/progenitor cells, suggesting a novel and uncharacterized effect of BrdU. This finding is significant in that BrdU-incorporating neural stem/progenitor cells and their progeny should not be expected to behave normally with respect to proliferative potential and downstream functional parameters. This effect highlights the need for caution when results based on long-term BrdU tracking over multiple rounds of replication are interpreted. Conversely, the reliable induction of senescence in stem/progenitor cells in vitro and in vivo may yield a novel platform for molecular studies designed to address multiple aspects of aging and neurogenesis.

show abstract

“…BrdU, a thymidine analog that is incorporated into the DNA of dividing cells during the S phase of the cell cycle, was used to label dividing cells (Struikmans et al ., ). Briefly, to evaluate the effects of EEPV on cell proliferation, BrdU (50 mg/kg of body weight dissolved in 0.02 M PBS, i.p.)…”

Section: Methodsmentioning

confidence: 97%

Standardized Prunella vulgaris var. lilacina Extract Enhances Cognitive Performance in Normal Naive Mice

Park

Ahn

Lee

et al. 2015

Phytotherapy Research

View full text Add to dashboard Cite

Adult hippocampal neurogenesis is closely associated with neuronal plasticity, cognitive function and the etiology of neurological diseases. We previously reported that the standardized ethanolic extract of Prunella vulgaris var. lilacina (EEPV) can be used for the prevention and treatment of cognitive impairments associated with Alzheimer's disease or schizophrenia. In the present study, we investigated the effects of EEPV on cognitive ability in normal naive mice and the underlying mechanism(s) governing these effects, including adult hippocampal neurogenesis. In the passive avoidance task, sub-chronic administration of EEPV (25 or 50 mg/kg, p.o.) for 14 days markedly induced the improvement of cognitive function in mice. In addition, sub-chronic administration of EEPV (25 or 50 mg/kg) for 14 days significantly increased neural cell proliferation and the number of immature neurons, but not newly generated cell survival, in the hippocampal dentate gyrus. Increased ERK, Akt and GSK-3β phosphorylation levels in the hippocampus were also observed after such administration. Our results indicate that EEPV may enhance cognitive function via the activation of various intracellular signaling molecules and the up-regulation of adult hippocampal neurogenesis.

show abstract

S-phase fraction, 5-bromo-2′-deoxy-uridine labelling index, duration of S-phase, potential doubling time, and DNA index in benign and malignant brain tumors

Cited by 20 publications

References 62 publications

Nuclear factor one B regulates neural stem cell differentiation and axonal projection of corticofugal neurons

Nuclear factor one B regulates neural stem cell differentiation and axonal projection of corticofugal neurons

Bromodeoxyuridine Induces Senescence in Neural Stem and Progenitor Cells

Standardized Prunella vulgaris var. lilacina Extract Enhances Cognitive Performance in Normal Naive Mice

Contact Info

Product

Resources

About