Ryanodine receptor point mutations confer diamide insecticide resistance in tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae)

Roditakis, Emmanouil; Steinbach, Denise; Moritz, Gerald; Vasakis, Emmanouil; Stavrakaki, Marianna; Ilias, Aris; García-Vidal, Lidia; Martínez-Aguirre, María del Rosario; Bielza, Pablo; Morou, Evangelia; Silva, Jefferson E.; Silva, Wellington Marcos da; Siqueira, Herbert A.A.; Iqbal, Sofia; Troczka, Bartlomiej J.; Williamson, Martin S.; Bass, Chris; Tsagkarakou, Anastasia; Vontas, John; Nauen, Ralf

doi:10.1016/j.ibmb.2016.11.003

Cited by 132 publications

(114 citation statements)

References 46 publications

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“…The potential damage and high growth rate of this pest have pushed growers to increase the number of insecticide applications throughout the tomato production cycle 1 . This has led to the rapid development of insecticide resistance 3,4 and has also had a considerable negative impact on non-target organisms, such as natural enemies and pollinators 5–7 . As a consequence, alternative control tools to conventional synthetic pesticides have been tested and/or implemented within tomato Integrated Pest Management (IPM) packages.…”

Section: Introductionmentioning

confidence: 99%

Citrus peel essential oil nanoformulations to control the tomato borer, Tuta absoluta: chemical properties and biological activity

Campolo

Cherif

Ricupero

et al. 2017

Sci Rep

135

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The repeated use of conventional synthetic pesticides in crop protection leads to resistance development by pests along with a negative impact on the environment, particularly non-target arthropods. Plant-derived active compounds, such as essential oils (EOs), play a key role in sustainably controlling pests. The lethal and sublethal activity of citrus peel EOs as emulsions and included in polyethylene glycol (PEG) nanoparticles (EO-NPs) was determined against the invasive tomato pest Tuta absoluta. Their effects on the plants were also assessed. The results showed an overall good insecticidal activity of the compounds tested, with a higher mortality through contact on eggs and larvae by EO emulsions and through ingestion on larvae by EO-NPs. The nanoformulation also significantly reduced the visible toxic effects on the plants. The data collected suggest that these natural compounds, especially when nanoformulated, could be successfully used in integrated pest management programs for T. absoluta.

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Section: Introductionmentioning

confidence: 99%

Citrus peel essential oil nanoformulations to control the tomato borer, Tuta absoluta: chemical properties and biological activity

Campolo

Cherif

Ricupero

et al. 2017

Sci Rep

135

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“…As in most cases of insecticide resistance, other studies have reported autosomal resistance of S. frugiperda , to lambda‐cyhalothrin, carbaryl, lufenuron and spinosad . For the diamide insecticides, the resistance of P. xylostella and T. absoluta was characterized as autosomal and incompletely recessive dominance …”

Section: Discussionmentioning

confidence: 99%

Selection and characterization of the inheritance of resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to chlorantraniliprole and cross‐resistance to other diamide insecticides

Bolzan

Padovez

Nascimento

et al. 2019

Pest Management Science

143

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BACKGROUND Understanding the genetic basis of insect resistance to insecticides can help to implement insecticide resistance management (IRM) strategies. In this study, we selected a strain of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) resistant to chlorantraniliprole using the F2 screen method, characterized the inheritance of resistance and evaluated patterns of cross‐resistance to other diamide insecticides. RESULTS The chlorantraniliprole‐resistant strain (Chlorant‐R) was selected from a field‐collected population with an estimated allele frequency of 0.1316. The estimated median lethal concentration (LC50) values were 0.011 and 2.610 µg a.i. cm–2 for the susceptible (Sus) and Chlorant‐R strains, respectively, resulting in a resistance ratio (RR) of 237‐fold. The LC50 values of the reciprocal crosses were 0.155 and 0.164 µg a.i. cm–2, indicating that resistance is autosomally inherited. Resistance was characterized as incompletely recessive and monogenic at concentrations close to the recommended field rates of chlorantraniliprole. Survival of the resistant strain and heterozygous larvae in maize plants treated at the field rate was ∼ 60 (similar to the susceptible strain on untreated maize) and 15%, respectively. The Chlorant‐R strain showed low cross‐resistance to cyantraniliprole (RR ∼ 27‐fold) and very high cross‐resistance to flubendiamide (RR > 42 000‐fold). CONCLUSIONS The frequency of chlorantraniliprole resistance allele was moderately high in a field‐collected population of S. frugiperda. The inheritance of chlorantraniliprole resistance was characterized as autosomal, incompletely recessive and monogenic. S. frugiperda showed cross‐resistance to other diamide insecticides. These results provide essential information for the implementation of IRM programs to preserve the useful life of diamide insecticides for controlling S. frugiperda in Brazil. © 2019 Society of Chemical Industry

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“…022, one can detect and monitor the resistance in short time in T. absoluta populations by detecting the mutations applying molecular assays and then sequencing the alleles. Such methods are briefly described for diamides, indoxacarb and spinosad by Roditakis, Steinbach et al (), Roditakis, Mavridis et al () and Silva et al (), respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Resistance to diamides has been related to mutations to the RyR of the insects. Specifically, Roditakis, Steinbach et al () found for the first time the mutation G4903E in the RyR of an Italian T. absoluta strain. The same authors comparing a susceptible strain and the strain from Italy using radioligand binding studies on thoracic membrane of T. absoluta adults confirmed the transmutation G4946E in the RyRs binding site where diamides attach.…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Cypriot Tuta absoluta populations to four targeted insecticides and control failure likelihood

Michaelides

Seraphides

Pitsillou

et al. 2019

J Applied Entomology

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Tuta absoluta, known as the South American tomato pinworm, is one of the most disastrous pests of tomato cultivations, presently menacing tomato cultivations worldwide. In 2006, T. absoluta invaded Spain from South America. Since then, it was rapidly spread to most European, African and Asian countries. Such alien invasive species can minimize crop production, whereas the increasing use of insecticides raises various environmental concerns as well as on control costs, control failure and the toxicity to non‐target organisms. The S. American tomato pinworm is mostly controlled by chemical insecticides, and failure to control it is not a rare phenomenon. Resistance to numerous insecticides has been reported and is mainly due to the fact that farmers do not follow a sustainable resistance management scheme. Several examples have been reported from several countries where the tomato pinworm is present. In order to develop a successful insecticide resistance management (IRM) strategy for any major pest, one needs to identify the baseline toxicity to insecticides and then monitor susceptibility levels. In Cyprus, the current status of susceptibility levels to the main insecticides that are used to control T. absoluta has never been studied before. Herein, nine Cypriot populations of the pest were subjected to laboratory bioassays between 2016 and 2018 using the main insecticides applied against it. We found that the insecticides chlorantraniliprole and indoxacarb could not control the Cypriot T. absoluta populations anymore, with a resistance ratio (RR) >28 and 3–23, respectively. Furthermore, mortality achieved by those two insecticides was 20.6%–72% for chlorantraniliprole and 27.5%–78% for indoxacarb. However, the insecticides emamectin benzoate and spinosad are very effective, since mortality to both of them ranged between 99.5% and 100%.

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Ryanodine receptor point mutations confer diamide insecticide resistance in tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae)

Cited by 132 publications

References 46 publications

Citrus peel essential oil nanoformulations to control the tomato borer, Tuta absoluta: chemical properties and biological activity

Citrus peel essential oil nanoformulations to control the tomato borer, Tuta absoluta: chemical properties and biological activity

Selection and characterization of the inheritance of resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to chlorantraniliprole and cross‐resistance to other diamide insecticides

Susceptibility of Cypriot Tuta absoluta populations to four targeted insecticides and control failure likelihood

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