Rutin synthase in fava d’anta: purification and influence of stressors

Lucci, Nathália; Mazzafera, Paulo

doi:10.4141/cjps09001

Cited by 24 publications

(14 citation statements)

References 25 publications

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“…This observation seemed to be a partial resistance for this legume and may be related to activation of the flavonol synthase (FLS) enzyme for biosynthesis of kaempferol and quercetin. In addition, the induced rutin may be fluxed from quercetin by rutin synthase enzyme, which activates this conversion under stress conditions (Lucci & Mazzafera, 2009). Similarly, accumulation of luteolin may originate from the decreasing apigenin through the flavonoid-3'-hydroxylase (F3'H) enzyme, which activates hydroxylation of apigenin into luteolin (Nakamura et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Flavonoid profiling and nodulation of some legumes in response to the allelopathic stress of Sonchus oleraceus L.

et al. 2015

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Annual sowthistle (Sonchus oleraceus) has been reported to produce allelopathic effects. Two greenhouse experiments were conducted to estimate the allelopathic potential of both plant residue and root exudates of S. oleraceus on flavonoid composition and nodulation in a leguminous crop, Trifolium alexandrinum, and in two leguminous weeds, Melilotus indicus and T. resupinatum. The results of high performance liquid chromatography-mass spectrometry (HPLC--MS/MS) showed that all three legumes contained six flavonoid aglycones: apigenin, daidzein, kaempferol, luteolin, myricetin and quercetin; and seven flavonoid glycosides: daidzin, genistin, hesperidin, hyperoside, kaempferol-7-O--glucoside, naringin and rutin. In general, both plant residue and root exudates had inhibitory effects on the flavonoid composition and nodulation of the target species. However, residue of S. oleraceus caused a significant increase in both individual and total detected flavonoids in T. alexandrinum. The results suggest that the phytotoxins released from S. oleraceus may restrain the biosynthesis of flavonoids in the target species, whereas the accumulated flavonoids in T. alexandrinum are allelopathic-induced metabolites and suggest a resistance mode in this crop.

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Section: Discussionmentioning

confidence: 99%

Flavonoid profiling and nodulation of some legumes in response to the allelopathic stress of Sonchus oleraceus L.

et al. 2015

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“…(buckwheat), Saussurea involucrata, and Eucalyptus macrorhyncha (Zielinska et al, 2010). It is also referred to as rutoside, quercetin-3-O-rutinoside, or sophorin, and it is the glycoside between the flavonol quercetin and the disaccharide rutinose ((α-L-rhamnopy-ranosyl-(1→6))-β-D-glucopyranose) (Lucci and Mazzafera, 2009). Several pharmacological activities have been attributed to rutin, and these include cytoprotective, antioxidant, anticarcinogenic, vasoprotective, and neuroprotective activities.…”

Section: Curcuma Longamentioning

confidence: 99%

The Potential for Plant Derivatives against Acrylamide Neurotoxicity

Adewale

Brimson

Odunola

et al. 2015

Phytotherapy Research

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Certain industrial chemicals and food contaminants have been demonstrated to possess neurotoxic activity and have been suspected to cause brain-related disorders in humans. Acrylamide (ACR), a confirmed neurotoxicant, can be found in trace amount in commonly consumed human aliments as a result of food processing or cooking. This discovery aroused a great concern in the public, and increasing efforts are continuously geared towards the resolution of this serious threat. The broad chemical diversity of plants may offer the resources for novel antidotes against neurotoxicants. With the goal of attenuating neurotoxicity of ACR, several plants extracts or derivatives have been employed. This review presents the plants and their derivatives that have been shown most active against ACR-induced neurotoxicity, with a focus on their origin, pharmacological activity, and antidote effects.

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“…This will provide the scientific basis for the quality control of extracts prepared from the inner bark of this plant. The choice of these three flavonoids was based on other studies, which reported the presence of these compounds in other species of Dimorphandra (Sousa et al, 1991;Ferreira et al, 2001;Lucci & Mazzafera, 2009). The method was validated according to regulation RE 899/2003 of the National Health Surveillance Agency, Brazil (Anvisa, 2003).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a HPLC method for the quantification of three flavonoids in a crude extract of Dimorphandra gardneriana

Landim¹,

Feitoza²,

Costa³

2013

Rev. bras. farmacogn.

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A method for separation and quantification of three flavonoids by reverse-phase high performance liquid chromatography (HPLC) was developed and validated. Flavonoids present in a crude methanolic extract of the inner bark of Dimorphandra gardneriana Tul., Fabaceae, were analyzed. Rutin, isoquercitrin and quercetin were used as calibration standards. The analysis was performed using a Thermo Scientific Hypersil C18 column (250 x 4.0 mm i.d., 5 μm particle size), as stationary phase, with a flow rate of 0.8 mL/min and detection at a wavelength of 356 nm. The proposed method was validated by resolution RE No. 899/2003 of the National Health Surveillance Agency. In this study, an excellent linearity was obtained with r higher than 0.99. Besides, the chromatographic peaks showed good resolution. With other validation data, including precision, specificity, accuracy and robustness, this method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of rutin, isoquercitrin and quercetin in crude methanolic extract of D. gardneriana pods. Furthermore, there are the advantages of easy sample preparation and short time between each injection.

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Rutin synthase in fava d’anta: purification and influence of stressors

Cited by 24 publications

References 25 publications

Flavonoid profiling and nodulation of some legumes in response to the allelopathic stress of Sonchus oleraceus L.

Flavonoid profiling and nodulation of some legumes in response to the allelopathic stress of Sonchus oleraceus L.

The Potential for Plant Derivatives against Acrylamide Neurotoxicity

Development and validation of a HPLC method for the quantification of three flavonoids in a crude extract of Dimorphandra gardneriana

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