Rutin- serum albumin interaction in different media and its effective dose selection in radiation-induced cytotoxicity on human blood cells

Effect of a Metal Ion in Modulating the Binding Interaction of a Dietary Flavonoid with Bovine Serum Albumin and DNA: A Spectroscopic and Theoretical Approach

“…Human peripheral blood mononuclear cells (PBMC) were isolated from whole blood (collected from healthy volunteers by venipuncture) as per the procedure described in our previously published report . Cells were then seeded on a 96-well plate and incubated at 37 °C with 5% CO 2 .…”

“…Next, cells were treated with different concentrations of free rutin and the rutin–Fe­(II) complex (0, 20, 40, 60, 80, 100, 120, and 140 μg/mL) for 6 h prior to exposure under UVB rays (10 mJ/cm 2 ). Immediately after the above-mentioned treatment, the MTT assay was performed in triplicate according to the protocol provided with the assay kit . The cytotoxic effect of each treatment was expressed as a percentage of viable cells corresponding to the untreated control cells and can be expressed as …”

“…Human peripheral blood mononuclear cells (PBMC) were isolated from whole blood (collected from healthy volunteers by venipuncture) as per the procedure described in our previously published report. 36 Cells were then seeded on a 96-well plate and incubated at 37 °C with 5% CO 2 . Next, cells were treated with different concentrations of free rutin and the rutin−Fe(II) complex (0, 20, 40, 60, 80, 100, 120, and 140 μg/mL) for 6 h prior to exposure under UVB rays (10 mJ/cm 2 ).…”

“…Immediately after the abovementioned treatment, the MTT assay was performed in triplicate according to the protocol provided with the assay kit. 36 The cytotoxic effect of each treatment was expressed as a percentage of viable cells corresponding to the untreated control cells and can be expressed as…”

“…A delicate comparison of the binding mechanism of the parent flavonoid, rutin, and its Fe­(II) complex with BSA/DNA is also lacking. In recent times, we have investigated the binding interaction of free rutin with model transport proteins, and its efficacy in ameliorating UVB radiation-induced cell damage has also been explored. , In this work, our goal has been to assess and compare the binding characteristics of free rutin and the rutin–Fe­(II) complex with BSA and DNA. Further, a comparative analysis of the antioxidant as well as radiation-induced damage protection activities of rutin and its Fe­(II) complex has been performed to recognize the beneficial effect of complexation.…”