Rutin inhibits human leukemia tumor growth in a murine xenograft model in vivo

Abstract

Numerous studies have shown that rutin has anticancer effects. We have previously reported that rutin induced cell cycle arrest and apoptosis in murine leukemia WEHI-3 cells in vitro and in vivo. However, there are no data showing that rutin inhibits human leukemia HL-60 cells in vivo in a murine xenograft animal model. Human leukemia HL-60 cells were implanted into mice and treated with vehicle (1% DMSO), rutin (120 mg/kg of body weight) or vinblastine (120 μg/kg of body weight). Compounds and agents were injected once every four days intraperitoneally (i.p.) for 36 days. Treatment with 120 mg/kg of rutin or with 120 μg/kg of vinblastine resulted in a reduction of tumor weight and volume when compared with the control groups. Tumor size in xenograft mice treated with 120 mg/kg of rutin was significantly smaller than that in the untreated-control group. These novel findings indicate that rutin inhibits tumor growth in a xenograft animal model. Rutin may be useful in treating leukemia but certainly much more research is needed. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2012.

Evaluation of mutagenic and antimutagenic activities of oligorutin and oligoesculin

“…In fact, it has been demonstrated that rutin causes cell cycle arrest and induces apoptosis in many types of human cancer cell lines (La Casa, Villegas, Alarcon de la Lastra, Motilva, & Martin Calero, 2000;Lin et al, 2009). On the other hand, some works have indicated that the enzymatic polymerisation of rutin might improve its biological activities (Anthoni et al, 2008;Kurisawa, Chung, Uyama, & Kobayashi, 2003).…”

“…Recently, attention has focussed on the identification of antimutagenic and/or anticarcinogenic effects of these polyphenols in animal systems (Lin et al, 2009). The mutagenic and antimutagenic activities of monomeric polyphenols were reported by some authors (Chulasiri, Bunyapraphatsara, & Moongkarndi, 1998).…”

Glycyrrhizic acid induces apoptosis in WEHI-3 mouse leukemia cells through the caspase- and mitochondria-dependent pathways

“…WEHI-3 cells at the density of 5x10 4 cells/well were plated on 4-well chamber slides and were then treated without (control) or with 300 µM of GA for 24 h. At the end of incubation, cells were washed with PBS and fixed with 4% formaldehyde in PBS for 15 min, followed by permeabilization for 1 h using 0.3% Triton X-100 in PBS containing 2% BSA for blocking non-specific binding sites. Cell samples were then stained by anti-AIF, anti-Endo G or anti-cytochrome c antibodies (1:100 dilution, respectively) for 24 h, washed twice with PBS and then stained with a secondary antibody (FITC-conjugated goat anti-mouse IgG at 1:100 dilution) for 40 min, followed by PI staining for DNA analysis.…”

“…According to the Department of Health, Executive Yuan, Taiwan R.O.C. (2010), approximately 4.2 per 100,000 people in Taiwan succumb to leukemia each year (3,4). Currently, treatment of leukemia in clinics includes hematopoietic stem cell transplantation, radiotherapy and chemotherapy agents; however, these outcomes are not fully satisfactory (5)(6)(7).…”

Polyphenols From Cutch Tree ( Acacia catechu Willd.): Normalize In Vitro Oxidative Stress and Exerts Antiproliferative Activity

“…Among these, ellagic acid, rutin and quercetin were found to be major polyphenolic compounds. The antioxidant and anticancer properties of these compounds have been well established [ 28,29,30,31,32 ]. The high level of protective activities imparted by these compounds serves as an evidence for the importance of AME extract in ameliorating the oxidative stress.…”