Ruthenium-Encapsulated Porphyrinic Organic Polymer as a Photoresponsive Oxidoreductase Mimetic Nanozyme for Colorimetric Sensing

Abstract

The advantages of porosity and stable unpaired electrons of porphyrinic organic polymers (POPs) with free radicals are exclusive and potentially practical functionalities and combining the semiconductor-like characteristics of these materials and metal ions has been an effective way to assemble an efficient photocatalytic system. Herein, a new ruthenium (Ru) ionencapsulated porphyrinic organic polymer (POP/Ru) is facilely synthesized as a proper photoresponsive nanozyme with unique photo-oxidase properties. Surprisingly, the proposed POP/Ru revealed outstanding photoresponsive oxidase-mimicking activity due to the synergetic effect of the integration of Ru and π-electrons of POP, which boosts charge separation and transport. POP/Ru was applied to the oxidation of o-phenylenediamine (o-PDA) as a chromogenic probe for producing a colorimetric signal. The kinetic study reveals that these photo-oxidase mimics have a significant affinity for the o-PDA chromogenic agent owing to a lower K m and superior V max . Further findings demonstrate that the presence of the Larginine (L-Arg) target causes an inhibition effect on the photo-nanozymatic colorimetry of POP/Ru. This research develops the applications of the comprehensive colorimetric strategy for ultrasensitive L-Arg monitoring with a limit of detection (LOD) of 15.2 nM in the dynamic range of 4.0 nM−340 μM and illuminates that the proposed photo-oxidase nanozyme as a visual strategy is feasible in L-Arg environmentally friendly colorimetric detection in juice samples.

Red-emissive carbon nanostructure-anchored molecularly imprinted Er-BTC MOF: a biosensor for visual anthrax monitoring

“…12 The remarkable importance of sensors lies in their ability to enable the engineering of a substrate with a specific design to detect and analyze the desired analyte, particularly under unique or specific conditions. 13,14 From another perspective, the advancement of public health relies on the monitoring of abnormalities in real samples, including body fluids and environmental waters, using biosensors. To fulfill this purpose, a wide range of luminescent materials have been actively explored for the development of biosensors.…”

Prussian Blue Analogues-Derived Molecularly Imprinted Nanozyme Array for Septicemia Detection

“…To investigate enzyme activity and establish the relationship between Fe–CeO 2 /MITiO 2 and enzyme steady-state kinetic parameters, a study of the Michaelis–Menten behaviors was conducted using TMB as the substrate. The Michaelis–Menten equation, V 0 = ( V max [S])/( K m + [S]), was employed to determine the initial velocity ( V 0 ), maximal reaction velocity ( V max ), the concentration of TMB substrate ([S]), and the Michaelis constant ( K m ). ,− By performing a series of nonenzymatic experiments on Fe–CeO 2 /MITiO 2 , the concentration of the TMB substrate was varied while keeping other parameters constant at different time points. The reaction rates for TMB oxidation were calculated, and this data was used to generate standard Michaelis–Menten curves (see Figure g).…”