Rupture of zwitterionic lipid vesicles by an amphipathic, α-helical peptide: Indirect effects of sensor surface and implications for experimental analysis

Zan, Goh Haw; Cho, Nam‐Joon

doi:10.1016/j.colsurfb.2014.06.014

Cited by 15 publications

(18 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The polybrene coating process of the silica surface was optimized in our previous capillary electrophoresis study [30]. In this work, the same coating procedure was utilized before each QCM [59,60]. This, on the other hand, suggests that the EggPC/POPG liposome layer adsorbed on the polybrene coated sensor surface is composed of highly truncated liposomes caused by the strong electrostatic interaction between the negatively charged liposomes and the positively charged polybrene surface.…”

Section: Quartz Crystal Microbalancementioning

confidence: 99%

Ionic liquids affect the adsorption of liposomes onto cationic polyelectrolyte coated silica evidenced by quartz crystal microbalance

Duša

Ruokonen

Petrovaj

et al. 2015

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

The worldwide use of ionic liquids (ILs) is steadily increasing, and even though they are often referred to as "green solvents" they have been reported to be toxic, especially toward aquatic organisms. In this work, we thoroughly study two phosphonium ILs; octyltributylphosphonium chloride ([P8444]Cl) and tributyl(tetradecyl)phosphonium chloride ([P14444]Cl). Firstly, the critical micelle concentrations (CMCs) of the ILs were determined with fluorescence spectroscopy and the optical pendant drop method in order to gain an understanding of the aggregation behavior of the ILs. Secondly, a biomimicking system of negatively charged unilamellar liposomes was used in order to study the effect of the ILs on biomembranes. Changes in the mechanical properties of adsorbed liposomes were determined by quartz crystal microbalance (QCM) measurements with silica coated quartz crystal sensors featuring a polycation layer. The results confirmed that both ILs were able to incorporate and alter the biomembrane structure. The membrane disrupting effect was emphasized with an increasing concentration and alkyl chain length of the ILs. In the extreme case, the phospholipid membrane integrity was completely compromised.

show abstract

Section: Quartz Crystal Microbalancementioning

confidence: 99%

Ionic liquids affect the adsorption of liposomes onto cationic polyelectrolyte coated silica evidenced by quartz crystal microbalance

Duša

Ruokonen

Petrovaj

et al. 2015

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

show abstract

“…One interest lies in the lipid vesicle fusion process that may take place during the intracellular delivery 17,18 . A lipid bilayer can under specific conditions be readily formed on top of the quartz sensor by vesicle spreading and rupturing.…”

Section: Introductionmentioning

confidence: 99%

Detection of Phase Transition in Photosensitive Liposomes by Advanced QCM

et al. 2015

View full text Add to dashboard Cite

In this work an impedance-based quartz crystal microbalance (QCM) is used to detect heat induced changes in the viscoelastic properties in the films of adsorbed liposomes. Liposomes are bound to a polymer-modified QCM surface, and heat is induced in the bilayer via light absorption into gold nanoparticles (GNPs) embedded in the liposomes. Due to very rapid heat transfer at the nanoscale, nanoparticles can reside either in the liposome cavity or within the bilayer to cause changes in the lipid viscoelasticity. The changes are observed as changes in the film relaxation time as well as by mapping the measured resonance frequency vs. resistance. The QCM results indicate that viscoelastic changes occur throughout the vesicle layer, possibly causing fusion between the liposomes. The ultimate goal of the work is to develop a smart drug delivery system for the eye, whereby a drug loaded in the liposome can be released in a controlled manner by light triggering.

show abstract

“…QCM‐D experiments provided key insights that revealed how peptide molecules adsorb onto the lipid membrane surface before inducing vesicle rupture, and the resulting vesicle‐to‐bilayer structural transformation provided a model system to study changes in thin‐film behavior . From a biosensing perspective, it was observed that the QCM‐D kinetics of the AH‐peptide‐mediated vesicle rupture process depend on the material composition of the sensor surface …”

Section: Bio‐membrane Nanoarchitectonics With Qcm‐dmentioning

confidence: 99%

“…[59] From ab iosensing perspective, it was observed that the QCM-D kinetics of the AHpeptide-mediated vesicle rupture process depend on the material composition of the sensorsurface. [60] However,t his AH peptideo nly ruptures adsorbed vesicles below ac ertain diameter,a nd QCM-Dm easurements identified that the efficiency of vesicle rupture was inversely relatedt o vesicle size. [61] Kinetic analysiso ft he QCM-D measurement data indicated that cooperative peptide binding leads to pore formation and vesicle rupture, whereas noncooperative binding does not cause vesicle rupture.…”

Section: Membrane-peptide Interactionsmentioning

confidence: 99%

Materials Nanoarchitectonics for Mechanical Tools in Chemical and Biological Sensing

Jackman

Cho

Nishikawa

et al. 2018

Chemistry An Asian Journal

View full text Add to dashboard Cite

In this Focus Review, nanoarchitectonic approaches for mechanical-action-based chemical and biological sensors are briefly discussed. In particular, recent examples of piezoelectric devices, such as quartz crystal microbalances (QCM and QCM-D) and a membrane-type surface stress sensor (MSS), are introduced. Sensors need well-designed nanostructured sensing materials for the sensitive and selective detection of specific targets. Nanoarchitectonic approaches for sensing materials, such as mesoporous materials, 2D materials, fullerene assemblies, supported lipid bilayers, and layer-by-layer assemblies, are highlighted. Based on these sensing approaches, examples of bioanalytical applications are presented for toxic gas detection, cell membrane interactions, label-free biomolecular assays, anticancer drug evaluation, complement activation-related multiprotein membrane attack complexes, and daily biodiagnosis, which are partially supported by data analysis, such as machine learning and principal component analysis.

show abstract

Rupture of zwitterionic lipid vesicles by an amphipathic, α-helical peptide: Indirect effects of sensor surface and implications for experimental analysis

Cited by 15 publications

References 33 publications

Ionic liquids affect the adsorption of liposomes onto cationic polyelectrolyte coated silica evidenced by quartz crystal microbalance

Ionic liquids affect the adsorption of liposomes onto cationic polyelectrolyte coated silica evidenced by quartz crystal microbalance

Detection of Phase Transition in Photosensitive Liposomes by Advanced QCM

Materials Nanoarchitectonics for Mechanical Tools in Chemical and Biological Sensing

Contact Info

Product

Resources

About