Ruminant cluster CD41/CD61

Mateo, A; Pintado, Carlos; Lastra, J. Perez de la; Dušinský, Roman; Simon, M.; Naessens, Jan; Llanes, D.

doi:10.1016/0165-2427(96)05570-5

Cited by 7 publications

(7 citation statements)

References 7 publications

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“…To reliably detect ovine platelets, a platelet specific marker is required. CAPP2A binds to CD41/61 on ovine platelets, and GB84A and GB20A bind to an 85-kD protein on the surface of bovine and ovine platelets (16,17). The antigen target for GB84A is CD42d, and this is also the presumed antigen target for GB20A, although no confirmation has been reported (personal communication, William C. Davis; 21).…”

Section: Discussionmentioning

confidence: 96%

“…After incubation, the samples were washed with 1 mL of Tyrode's buffer containing 1% BSA and 0.106-M sodium citrate and centrifuged at 132 × g for 10 min. The supernatant was then removed and the pellet was resuspended.CAPP2A is an antibody that recognizes GpIIbIIIa (CD41/61), an antigen on the surface of resting ovine platelets (16,17). N-hydroxysulfosuccinimide-LC-LC-biotin (LC refers to a hydrocarbon chain extender to reduce steric hindrance; Pierce, Rock-ford, IL, USA) was added to CAPP2A in 20-molar excess to produce CAPP2A-biotin for use as a platelet marker.…”

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confidence: 99%

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Flow Cytometric Assays for Quantifying Activated Ovine Platelets

et al. 2007

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Ovines are a common animal model for preclinical evaluation of cardiovascular devices including heart valves, endovascular grafts, and ventricular assist devices. Biocompatibility is essential to the success of these devices; however, tools to assess biocompatibility in ovines are limited. To address this need, antibodies that bind to activated human and bovine platelets and annexin V protein were evaluated for potential cross-reactivity to activated ovine platelets. These candidate markers were incubated with stimulated and quiescent ovine whole blood, and binding to platelets was quantified by flow cytometry. Several antihuman CD62P antibodies including one polyclonal antibody, three monoclonal antibodies, and annexin V selectively bound to activated ovine platelets. An assay to quantify platelet microaggregates was also developed. The availability of assays to quantify ovine platelet activation can increase the quality of biocompatibility data obtainable during preclinical development of artificial organs in the ovine model, potentially aiding in the evaluation of design refinements to enhance device biocompatibility. KeywordsBiocompatibility; Flow cytometry; Ovine platelets; Cardiovascular devices; Platelet activation Ovines are a common animal model for preclinical testing of blood-contacting cardiovascular devices including mechanical heart valves, endovascular grafts, and ventricular assist devices (VADs) (1-4). A critical aspect in the design of these devices is the evaluation of their blood biocompatibility. Yet, the biocompatibility data that can be obtained in ovine studies is limited due to a paucity of available assays for evaluating circulating blood elements during the implant period. In particular, there has only been one report on the use of flow cytometry to evaluate ovine platelet activation in platelet-rich plasma, and there have been no reports in the literature using such techniques to assess temporal platelet activation in ovines implanted with cardiovascular devices (5).Flow cytometry permits surface expression of platelet activation-dependent epitopes to be quantified, providing insight on circulating platelets not obtainable with platelet aggregometry and plasma assays for β-thromboglobulin and platelet factor 4 (6). Circulating activated platelets have been measured in patients with stents, mechanical heart valves, and VADs as well as in patients suffering from acute myocardial infarction and ischemic stroke [7][8][9][10][11][12][13]. The presence of circulating activated platelets has been suggested as a marker for increased risk of thrombotic complications (10). Previously, we have described several flow cytometric assays to quantify circulating activated bovine platelets and platelet microaggregates (14). These assays have been applied to assess circulating activated platelets in calves implanted with rotary VADs (14,15). The results demonstrate the ongoing presence of circulating activated platelets after the effects of surgery (without extracorporeal circulation) have di...

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Section: Discussionmentioning

confidence: 96%

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confidence: 99%

Flow Cytometric Assays for Quantifying Activated Ovine Platelets

et al. 2007

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“…A previous report supports this rationale, suggesting that CAPP2A may cross-react with granulocytes and monocytes. 13 When PRP was used in our study instead of whole blood, microaggregate formation in response to platelet agonists was readily quantifiable. In verifying this phenomenon, the percentage of microaggregates detected by flow cytometry with CAPP2A agreed well with the percentage observed visually with phase-contrast microscopy.…”

Section: Discussionmentioning

confidence: 99%

Flow cytometric assays to detect platelet activation and aggregation in device-implanted calves

Baker

Davis

Autieri

et al. 1998

J. Biomed. Mater. Res.

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Cardiovascular device development often relies upon large-animal models to assess blood biocompatibility prior to initiating clinical trials. Unfortunately, the amount of information gleaned from such trials is limited by simple assays that do not take full advantage of immunotechnological advances that increasingly are applied in clinical studies. Thus we have developed and tested new flow cytometric techniques for measuring circulating activated bovine platelets and platelet microaggregates. Monoclonal antibodies (MAbs) raised against both activated and quiescent bovine platelets were incubated with control and PMA-or ADP-stimulated whole blood. Selected MAbs detected activated bovine platelets and platelet microaggregates in vitro with flow cytometry. Five calves implanted with one of two designs of nonpulsatile ventricular-assist devices (VADs) were followed with these assays prior to and during VAD implantation. Circulating activated bovine platelets and microaggregates increased after implantation in all animals and, alternatively, remained elevated or returned toward preimplant levels. Platelet activation percentages as detected temporally by three MAbs were correlated with one another, and platelet activation was correlated with microaggregate formation. In summary, these new methods for the sensitive measurement of circulating activated bovine platelets and microaggregates may provide valuable information for the development and assessment of future cardiovascular device designs.

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“…This has been previously observed and may be because of adherence of activated platelets or platelet fragments to the cells. 44,45 No RBC-specific markers were available, but because they were negative for CD45 and CD41/ CD61, the isolated population was assumed to be pure.…”

Section: Staining For Flow Cytometry: Ruminant Studiesmentioning

confidence: 99%

Comparative analysis of normal prion protein expression on human, rodent, and ruminant blood cells by using a panel of prion antibodies

Barclay

Houston²,

Halliday³

et al. 2002

Transfusion

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Different animal species have unique patterns of expression of PrPC on blood cell types, with none equivalent to the human pattern. This needs to be considered when extrapolating from animal models of blood-borne transmissible spongiform encephalopathy infectivity, particularly in regard to the risk assessment of potential variant CJD spread within the human population. The relationship between PrP distribution and infectivity distribution in blood needs further investigation.

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Ruminant cluster CD41/CD61

Cited by 7 publications

References 7 publications

Flow Cytometric Assays for Quantifying Activated Ovine Platelets

Flow Cytometric Assays for Quantifying Activated Ovine Platelets

Flow cytometric assays to detect platelet activation and aggregation in device-implanted calves

Comparative analysis of normal prion protein expression on human, rodent, and ruminant blood cells by using a panel of prion antibodies

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