Rufibacter tibetensis gen. nov., sp. nov., a novel member of the family Cytophagaceae isolated from soil

Abstract

A red-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium which was motile by gliding, designated strain 1351(T), was isolated from the soil of Lengduo, Tibet in China and subjected to a polyphasic taxonomic analysis. The isolate grows optimally at 30°C and pH 7. It grows with NaCl tolerated up to 1.5% (optimum, 0.5%). Phylogenetic analysis based on the 16S rRNA gene sequence shows that strain 1351(T) is closely related to members of the family 'Cytophagaceae' closest sequence similarity was observed with similarity with Adhaeribacter terreus (91.8%). The major whole-cell fatty acids are summed feature 4 (containing anteiso-C(17:1) B and/or iso-C(17:1) I, 29.2%), summed feature 3 (containing C(16:1)ω6c and/or C(16:1)ω7c, 13.0%) and iso-C(15:0) (12.0%). The predominant menaquinone of strain 1351(T) is menaquinone-7 (MK-7) and the G+C content of the DNA is 46.8 mol%. According to the phylogenetic evidence and phenotypic data, strain 1351(T) is considered to represent a new genus and species of the family 'Cytophagaceae' for which the name Rufibacter tibetensis gen nov., sp. nov. is proposed. The type species is R. tibetensis and the type strain is 1351(T) (=CCTCC AB 208084(T) = NRRL B-51285(T)).

Rufibacter roseus sp. nov., isolated from radiation-polluted soil

“…Flagella were not observed but irregular extracellular structures occurred. Moreover, the long and irregular extracellular structures observed in strain H359 T appeared different to the irregular fibrillar structures reported for R. tibetensis CCTCC AB 208084 T (Abaydulla et al , 2012). Colonies were circular, convex, shiny, rose and smooth on R2A agar after 3 days at 30 °C.…”

“…Their main respiratory quinone is menaquinone 7 (MK-7) (Nakagawa, 2011). The genus Rufibacter was isolated from Tibet during the course of exploring novel radiation-resistant bacteria to investigate their carotenoids, which may be used as antioxidants (Abaydulla et al , 2012). At the time of writing, only one species with a validly published name is recognized as a member of the genus Rufibacter (Abaydulla et al , 2012).…”

“…The genus Rufibacter was isolated from Tibet during the course of exploring novel radiation-resistant bacteria to investigate their carotenoids, which may be used as antioxidants (Abaydulla et al , 2012). At the time of writing, only one species with a validly published name is recognized as a member of the genus Rufibacter (Abaydulla et al , 2012). During an investigation of microbial sources in the Xinjiang Uygur Autonomous Region of PR China, a novel bacterium, designated H359 T , was isolated from a soil sample contaminated by Cs 137 .…”

Rufibacter glacialis sp. nov., a psychrotolerant bacterium isolated from glacier soil

“…Menaquinone-7 (MK-7) is the major respiratory menaquinone and phosphatidylethanolamine is the predominant polar lipid of the genus Rufibacter. The genus comprises, at the time of writing, three recognized species (Abaydulla et al, 2012;Polkade et al, 2015; Zhang et al, 2015). During a study of bacterial diversity on glaciers in China, a strain producing red colonies (MDT1-10-3 T ) was isolated from soil collected from Midui glacier, Tibet, China.…”

“…The genus Rufibacter was originally created by Abaydulla et al (2012), and comprises aerobic Gram-stain-negative, catalase-and oxidase-positive, non-spore-forming bacteria. Menaquinone-7 (MK-7) is the major respiratory menaquinone and phosphatidylethanolamine is the predominant polar lipid of the genus Rufibacter.…”