Rufibacter glacialis sp. nov., a psychrotolerant bacterium isolated from glacier soil

Abstract

A Gram-stain-negative, rod-shaped, red-pigmented bacterium (MDT1-10-3 T ) was isolated from Midui glacier in Tibet, China. Cells were aerobic and psychrotolerant (growth occurred at 4-25 8C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that it was a member of the genus Rufibacter, with Rufibacter immobilis MCC P1 T (96.7 % similarity) as its closest phylogenetic relative. MK-7 was the predominant respiratory menaquinone. The major cellular fatty acids were summed feature 4 (iso-C 17 : 1 I and/or anteiso-C 17 : 1 B), summed feature 3 (C 16 : 1 v6c and/or C 16 : 1 v7c), C 17 : 1 v6c, iso-C 16 : 0 , iso-C 15 : 0 and C 16 : 1 v5c. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, one glycolipid and four unidentified lipids. The G+C content of the genomic DNA was 49 mol%. On the basis of the phenotypic characteristics and phylogenetic analysis, strain MDT1-10-3 T represents a novel species of the genus Rufibacter, for which the name Rufibacter glacialis sp. nov. is proposed. The type strain is MDT1-10-3 T (5CGMCC 1.9789 T 5NBRC 109705 T ).The genus Rufibacter was originally created by Abaydulla et al. (2012), and comprises aerobic Gram-stain-negative, catalase-and oxidase-positive, non-spore-forming bacteria. Menaquinone-7 (MK-7) is the major respiratory menaquinone and phosphatidylethanolamine is the predominant polar lipid of the genus Rufibacter. The genus comprises, at the time of writing, three recognized species (Abaydulla et al., 2012;Polkade et al., 2015; Zhang et al., 2015). During a study of bacterial diversity on glaciers in China, a strain producing red colonies (MDT1-10-3 T ) was isolated from soil collected from Midui glacier, Tibet, China. In this study, we describe the morphological, biochemical and phylogenetic characteristics of strain MDT1-10-3 T , employing the closely related type strains Rufibacter immobilis CCTCC AB 2013351 T and Rufibacter tibetensis CCTCC AB 208084 T as reference strains in parallel tests for phenotypic and fatty acid analysis. The reference strains were obtained from the Chinese Centre for type Cultures Collections.Strain MDT1-10-3 T was isolated using R2A agar at 14 8C and stored at 280 8C in 20 % (v/v) glycerol. Colony morphology was observed on R2A agar after incubation at 20 8C for 5 days. Growths at different temperatures (4, 14, 18, 20, 22, 25, 30 8C), different NaCl concentrations [0-4 % (w/v), at intervals of 1 %] and different pH (4.0-12.0, at intervals of 1.0 pH unit) were examined in R2A broth. The R2A broth used to test pH range was adjusted by using the biological buffers Na 2 HPO 4 / NaH 2 PO 4 (for pH 4-8) and Na 2 CO 3 /NaHCO 3 (for pH 9-12), and filter-sterilized. Cell morphology and dimensions were observed using a JEM

Jeotgalibacillus Auranticolor sp. nov., Isolated from Freshwater Collected from Baiyangdian Lake Lake, China

“…The cell wall was prepared, and the peptidoglycan structure was determined as described previously (Schleifer and Kandler, 1972). The cellular fatty acid properties of strain R-1-5s-1 T , Azospirillum doebereinerae GSF71 T , and Azospirillum lipoferum59b T were evaluated in parallel using a 6890N gas chromatograph (Agilent Technologies, Santa Clara, CA), and analyzed against the TSBA v. 6.0 database (Liu et al, 2016). The polar lipid and respiratory quinone properties of strain R-1-5s-1 T , J. salarius R-1-5s-1 T , and ASL-1 T were examined in parallel.…”

Rufibacter hautae sp. nov., a red-pigmented bacterium from freshwater lake sediment, and proposal of Rufibacter quisquiliarum as a latter heterotypic synonym of Rufibacter ruber

“…ruber from fragmentary rock [6], R. glacialis from glacier soil [7], R. quisquiliarum from bioreactor treating landfill leachate [8] and R.…”

“…Members of this genus are Gram-stainnegative, rod-shaped, non-spore-forming, with menaquinone 7 (MK-7) as the major respiratory quinone and phosphatidylethanolamine as the predominant polar lipid. Currently, the genus Rufibacter comprises seven species with validly published names: R. tibetensis from soil [1], R. roseus from radiation-polluted soil [4], R. immobilis from saline lake [5], R. ruber from fragmentary rock [6], R. glacialis from glacier soil [7], R. quisquiliarum from bioreactor treating landfill leachate [8] and R. sediminis from lake sediment [9]. The strains R. ruber and R. quisquiliarum were published in 2016 and the ANI and dDDH values between them were 98.0 and 83.0 %, respectively, which was all above the accepted cut-off [10].…”

Rufibacter latericius sp. nov., isolated from Baiyang Lake

“…The cellular fatty acids were extracted and analysed using a standard midi 6.0 system. An Agilent Technologies 6890 N gas chromatograph and the TSBA 6.0 database were used to identify the cellular fatty acids [6]. For polar lipid and respiratory quinone chemotaxonomic analyses of strain R-22-1 c-1 T , the related type strain R. immobilis MCC P1 T were tested in parallel.…”