Rubus rosifolius varieties as antioxidant and potential chemopreventive agents

Campbell, Theresa F.; McKenzie, Jacinth A.M.; Murray, JeAnn; Delgoda, Rupika; Bowen‐Forbes, Camille S.

doi:10.1016/j.jff.2017.07.040

Cited by 19 publications

(13 citation statements)

References 36 publications

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“…Ellagic acid (m/z 300.9990), which was detected in Ese, belongs to the class of organic compounds known as ellagitannin. This compound is well known for its antioxidant activity with free radical scavenging properties [19]. Ellagitannin compounds were found in both samples, although galloyl-HHDP-hexoside (m/z 633.0559) was detected only in F3.…”

Section: Discussionmentioning

confidence: 91%

Anti-inflammatory and antioxidant potential, in vivo toxicity, and polyphenolic composition of Eugenia selloi B.D.Jacks. (pitangatuba), a Brazilian native fruit

et al. 2020

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Brazilian native fruits are a rich source of polyphenolic compounds that can act as antiinflammatory and antioxidant agents. Here, we determined the polyphenolic composition, anti-inflammatory mechanism of action, antioxidant activity and systemic toxicity in Galleria mellonella larvae of Eugenia selloi B.D.Jacks. (synonym Eugenia neonitida Sobral) extract (Ese) and its polyphenol-rich fraction (F3) obtained through bioassay-guided fractionation. Phenolic compounds present in Ese and F3 were identified by LC-ESI-QTOF-MS. The anti-inflammatory activity of Ese and F3 was tested in vitro and in vivo through NF-κB activation, cytokine release and neutrophil migration assays. The samples were tested for their effects against reactive species (ROO•, O2•-, HOCl and NO•) and for their toxicity in Galleria mellonella larvae model. The presence of hydroxybenzoic acid, ellagitannins and flavonoids was identified. Ese and F3 reduced NF-κB activation, cytokine release and neutrophil migration, with F3 being threefold more potent. Overall, F3 exhibited strong antioxidant effects against biologically relevant radicals, and neither Ese nor F3 were toxic to G. mellonella larvae. In conclusion, Ese and F3 revealed the presence of phenolic compounds that decreased the inflammatory parameters evaluated and inhibited reactive oxygen/nitrogen species. E. selloi is a novel source of bioactive compounds that may provide benefits for human health.

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Section: Discussionmentioning

confidence: 91%

Anti-inflammatory and antioxidant potential, in vivo toxicity, and polyphenolic composition of Eugenia selloi B.D.Jacks. (pitangatuba), a Brazilian native fruit

et al. 2020

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“…Compound 1 underwent spontaneous oxidation with atmospheric oxygen to form a flaky white solid with a rancid odor. The degradation products were analyzed using NMR spectroscopy and GC-MS. Each solvent extract (n-hexane, EtOAc, and MeOH) from the two R. rosifolius varieties was analyzed in a preliminary oral glucose tolerance test (OGTT) study, and based on other studies conducted on the extracts from the two fruit varieties, R Hex , WR EtOAc , and R MeOH were the most promising and were therefore the focus of this study (Campbell et al, 2017). The hypoglycemic effects of the crude extracts were assessed using a previously reported OGTT with minor modifications (Alexander-Lindo, Salmon, & McGrowder, 2017).…”

Section: Isolation and Analysis Of Compoundmentioning

confidence: 99%

“…Two varieties of Rubus rosifolius, "Red" and "Wine Red" (R and WR, respectively), have been described by our research group (Rambaran & Bowen-Forbes, 2020). Their phytochemistry along with their antioxidant and cytochrome P450 (CYP) inhibitory activity were determined and they were found to possess potential chemopreventive activity (Campbell, McKenzie, Murray, Delgoda, & Bowen-Forbes, 2017). To the best of our knowledge, the antidiabetic or antihypoglycemic activity of these R. rosifolius plants however has not been reported to date.…”

mentioning

confidence: 99%

“…We also examined possible mechanisms for the oxidation of a mixture of triacylglycerols (TAGs). The extraction protocol was previously reported (Campbell et al, 2017). Briefly, ripe R. rosifolius berries were freeze-dried using a Labconco Freezone Freeze Dryer system (Kansas City, MO, USA) and a specific mass of each (160 g and 202 g for the R and WR fruit powders, respectively) extracted successively with n-hexane, ethyl acetate, and methanol, with a solvent to plant ratio of 4:1 (v/w).…”

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confidence: 99%

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Hypoglycemic effect of the fruit extracts of two varieties of Rubus rosifolius

et al. 2020

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Oral glucose tolerance test on male Sprague–Dawley rats was done to determine the hypoglycemic effect of the n‐hexane, ethyl acetate and methanol fruit extracts (50 mg/kg BW; oral administration) of two varieties of Rubus rosifolius. Metformin was used as a positive control (15 mg/kg BW; intravenous administration). The n‐hexane extract was most potent and was investigated phytochemically to yield compound 1, a mixture of triacylglycerols. In its oxidized state compound 1 produced a significant hypoglycemic effect which was more effective than metformin for the first 30 min of the assay (p = .03) and not significantly different for up to 120 min. Mechanisms through which the oxidized triacylglycerol species could form were explored and presented. This is the first account of the hypoglycemic activity of R. rosifolius and it is also the first account of this activity being credited to compounds other than polyphenols and terpenes in Rubus plants. Practical applications A significant percentage of the world’s population is affected by diabetes and diabetes‐related illnesses. One of the most popular antidiabetic drug on the market is metformin which is used to lower blood glucose concentrations. The findings of this study indicate that an oxidized mixture of triacylglycerols is more fast‐acting than metformin for the first 30 min of an oral glucose tolerance test. This effect was also not significantly different from that of the popular drug up to 120 min. These results demonstrate the hypoglycemic activity of an oxidized R. rosifolius fruit extract and indicate its potential use in applications such as functional food product development and drug discovery. Therapeutic applications can also include the plant extract as a potential primary treatment or as adjunct therapy to conventional medications.

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“…Em relação à desativação dessa ERO, pode-se observar que as frutas cambuití-cipó e murici vermelho apresentaram as maiores atividades, ou seja, os menores valores de EC50 (4,99 e 4,41 µg mL -1 , respectivamente SANNOMIYA et al, 2005). Também é a primeira vez que se relata a catequina na espécie araçá-boi, catequina e epicatequina e seus derivados no murici vermelho (SAMPAIO et al, 2015) e no morango silvestre (HASSIMOTTO et al, 2008;JAKOBEK et al, 2009;CAMPBELL et al, 2017). A epicatequina-3-O-gallato foi identificada pela primeira vez no araçá-boi, e no seu gênero Eugenia, além do murici vermelho.…”

Section: Análise Estatísticaunclassified

Composição fenólica e atividade biológica <em>in vitro</em> e <em>in vivo</em> de frutas nativas brasileiras

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Rubus rosifolius varieties as antioxidant and potential chemopreventive agents

Cited by 19 publications

References 36 publications

Anti-inflammatory and antioxidant potential, in vivo toxicity, and polyphenolic composition of Eugenia selloi B.D.Jacks. (pitangatuba), a Brazilian native fruit

Anti-inflammatory and antioxidant potential, in vivo toxicity, and polyphenolic composition of Eugenia selloi B.D.Jacks. (pitangatuba), a Brazilian native fruit

Hypoglycemic effect of the fruit extracts of two varieties of Rubus rosifolius

Composição fenólica e atividade biológica <em>in vitro</em> e <em>in vivo</em> de frutas nativas brasileiras

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