Rubus imperialis (Rosaceae) extract and pure compound niga-ichigoside F1: wound healing and anti-inflammatory effects

Tonin, Talita Dacroce; Thiesen, Liliani Carolini; Nunes, Maria Luisa de Oliveira; Broering, Milena Fronza; Donato, Marcos Paulo; Goss, Marina Jagielski; Petreanu, Marcel; Niero, Rivaldo; Machado, Isabel Daufenback; Santin, José Roberto

doi:10.1007/s00210-016-1285-8

Cited by 15 publications

(6 citation statements)

References 40 publications

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“…In addition to experiments on wound healing, Tonin et al [ 66 ] evaluated the anti-inflammatory activity of R. imperialis methanol extract from aerial parts. The extract decreased nitric oxide release by neutrophils from BALB/c mice, inflamed by intraperitoneal injection of oyster glycogen.…”

Section: Resultsmentioning

confidence: 99%

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A Review of the Potential Benefits of Plants Producing Berries in Skin Disorders

et al. 2020

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During the last 30 years, berries have gained great attention as functional food against several risk factors in chronic diseases. The number of related publications on Pubmed rose from 1000 items in 1990 to more than 11,000 in 2019. Despite the fact that a common and clear definition of “berries” is not shared among different scientific areas, the phytochemical pattern of these fruits is mainly characterized by anthocyanins, flavanols, flavonols, and tannins, which showed antioxidant and anti-inflammatory properties in humans. Skin insults, like wounds, UV rays, and excessive inflammatory responses, may lead to chronic dermatological disorders, conditions often characterized by long-term treatments. The application of berries for skin protection is sustained by long traditional use, but many observations still require a clear pharmacological validation. This review summarizes the scientific evidence, published on EMBASE, MEDLINE, and Scholar, to identify extraction methods, way of administration, dose, and mechanism of action of berries for potential dermatological treatments. Promising in vitro and in vivo evidence of Punica granatum L. and Vitis vinifera L. supports wound healing and photoprotection, while Schisandra chinensis (Turcz.) Baill. and Vaccinium spp. showed clear immunomodulatory effects. Oral or topical administrations of these berries justify the evaluation of new translational studies to validate their efficacy in humans.

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Section: Resultsmentioning

confidence: 99%

“…On the other end, Tonin at al. [ 66 ] evaluated the effect of aerial parts (leaves and branches) of R. imperialis , extracted by methanol maceration. In analogy with mentioned inflammation experiments, the biological activity at 1–100 μg/mL was compared with triterpenoid saponin niga-ichigoside F1 (1–100 μM).…”

Section: Resultsmentioning

confidence: 99%

A Review of the Potential Benefits of Plants Producing Berries in Skin Disorders

et al. 2020

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“…To remove debrided cells, the wells were washed with sterile phosphate-buffered saline (PBS; NaCl 137 mM, KCl 3 mM, KH 2 PO 4 1.5 mM, and Na 2 HPO 4 10 mM), followed by the addition of COS1 and COS5 at different concentrations (100, 250, and 500 µg/mL) and fetal bovine serum (10%). After the treatment, the wells were monitored using photographs at 0, 6, 12, 18, and 24 h to assess the cell migration towards the free space resulting from the horizontal risk (NIS Elements AR) [ 65 , 66 ].…”

Section: Methodsmentioning

confidence: 99%

Production and Characterization of Chitooligosaccharides: Evaluation of Acute Toxicity, Healing, and Anti-Inflammatory Actions

Andrade

Araújo

Torres-Rêgo

et al. 2021

IJMS

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The search for promising biomolecules such as chitooligosaccharides (COS) has increased due to the need for healing products that act efficiently, avoiding complications resulting from exacerbated inflammation. Therefore, this study aimed to produce COS in two stages of hydrolysis using chitosanases derived from Bacillus toyonensis. Additionally, this study aimed to structurally characterize the COS via mass spectrometry, to analyze their biocompatibility in acute toxicity models in vivo, to evaluate their healing action in a cell migration model in vitro, to analyze the anti-inflammatory activity in in vivo models of xylol-induced ear edema and zymosan-induced air pouch, and to assess the wound repair action in vivo. The structural characterization process pointed out the presence of hexamers. The in vitro and in vivo biocompatibility of COS was reaffirmed. The COS stimulated the fibroblast migration. In the in vivo inflammatory assays, COS showed an antiedematogenic response and significant reductions in leukocyte migration, cytokine release, and protein exudate. The COS healing effect in vivo was confirmed by the significant wound reduction after seven days of the experiment. These results indicated that the presence of hexamers influences the COS biological properties, which have potential uses in the pharmaceutical field due to their healing and anti-inflammatory action.

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“…The animals were anaesthetized, and their backs were shaved with an electric clipper. Then, a surgical excision of the skin (1.5 cm of diameter) was made on the dorsal area of each mouse, as previously described by Tonin et al [25]. The extract was topically applied to each wound (once a day, for 7 days).…”

Section: Efferocytosis Assaymentioning

confidence: 99%

Anti-Inflammatory and Healing Activity of the Hydroalcoholic Fruit Extract of Solanum diploconos (Mart.) Bohs

Benvenutti

Nunes

Venturi

et al. 2021

Journal of Immunology Research

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Background. Solanum diploconos (Mart.) Bohs is a native Brazilian plant belonging to the Solanaceae family, popularly known as “tomatinho do mato” and poorly investigated. Herein, we presented for the first time evidence for the anti-inflammatory and wound healing activities of S. diploconos fruit hydroalcoholic extract. Material and Methods. In vitro fMLP-induced chemotaxis, LPS-induced inflammatory mediator levels (cytokines by ELISA and NO release by Griess reaction), and adhesion molecule expression (CD62L, CD49d, and CD18, by flow-cytometry) were assessed in neutrophils treated with different concentrations of the extract. Inflammation resolution was measured by the efferocytosis assay and the healing activity by in vivo and in vitro assays. The air pouch model of carrageenan-induced inflammation in Swiss mice was used to investigate the in vivo anti-inflammatory effects of the extract. Leukocyte influx (by optical microscopy) and cytokine release were quantified in the pouch exudates. Additionally, the acute and subacute toxic and genotoxic effects of the extract were evaluated. Results. In vitro, the extract impaired neutrophil chemotaxis and its ability to produce and/or release cytokines (TNFα, IL-1β, and IL-6) and NO upon LPS stimuli ( p < 0.01 ). LPS-treated neutrophils incubated with the extract presented increased CD62L expression ( p < 0.01 ), indicating a reduced activation. An enhanced efferocytosis of apoptotic neutrophils by macrophages was observed and accompanied by higher IL-10 and decreased TNFα secretion ( p < 0.01 ). In vivo, similar results were noted, including reduction of neutrophil migration, protein exudation, and cytokine release ( p < 0.01 ). Also, the extract increased fibroblast proliferation and promoted skin wound healing ( p < 0.01 ). No signs of toxicity or genotoxicity were observed for the extract. Conclusion. S. diploconos fruit extract is anti-inflammatory by modulating neutrophil migration/activation as well macrophage-dependent efferocytosis and inflammatory mediator release. It also indicates its potential use as a healing agent. Finally, the absence of acute toxic and genotoxic effects reinforces its possible use as medicinal product.

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Rubus imperialis (Rosaceae) extract and pure compound niga-ichigoside F1: wound healing and anti-inflammatory effects

Cited by 15 publications

References 40 publications

A Review of the Potential Benefits of Plants Producing Berries in Skin Disorders

A Review of the Potential Benefits of Plants Producing Berries in Skin Disorders

Production and Characterization of Chitooligosaccharides: Evaluation of Acute Toxicity, Healing, and Anti-Inflammatory Actions

Anti-Inflammatory and Healing Activity of the Hydroalcoholic Fruit Extract of Solanum diploconos (Mart.) Bohs

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