Rubritalea profundi sp. nov., isolated from deep-seawater and emended description of the genus Rubritalea in the phylum Verrucomicrobia

Abstract

A Gram-stain-negative, short-rod, facultatively anaerobic, non-motile and red-pigmented bacterium, designated SAORIC-165, was isolated from a deep-seawater sample collected from the Pacific Ocean. The 16S rRNA gene sequence analysis showed that strain SAORIC-165 was most closely related to Rubritalea marina Pol012 (95.7 % sequence similarity) and formed a robust phylogenetic clade with other species of the genus Rubritalea in the phylum Verrucomicrobia. Optimal growth of strain SAORIC-165 was observed at 10 °C, pH 7.0 and in the presence of 2.0-3.5 % (w/v) NaCl. The DNA G+C content of strain SAORIC-165 was 50.7 mol% and MK-9 was the predominant isoprenoid quinone. The major cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C14 : 0, anteiso-C15 : 0, C16 : 0 and C14 : 0. The major polar lipids constituted phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified phospholipids and aminolipids. On the basis of the taxonomic data obtained in this study, it was concluded that strain SAORIC-165 represented a novel species of the genus Rubritalea, for which the name Rubritalea profundi sp. nov. is proposed. The type strain of Rubritalea profundi is SAORIC-165 (=NBRC 110691=KCTC 52460).

Water temperature and disease alters bacterial diversity and cultivability from American lobster (Homarus americanus) shells

“…We isolated Gordonia only from lobsters without shell disease and which did not die, which agrees with other findings in which Gordonia was only present on red rock lobsters ( Jasus edwardsii ) without tail fin necrosis. 54 Rubritalea 55 , 56 and Gordonia 54 , 57 species have been identified in marine systems and associated commensals on the shells of marine animals where they produce carotenoids, which might act as antimicrobials, although we did not examine this possibility. Carotenoids in lobster shells are typically sourced from plankton and other dietary components, but it is possible that microbially produced carotenoids on the surface of shells are serving to drive shell communities.…”

Effects of Elevated pCO2 on the Survival and Growth of Portunus trituberculatus

“…The genus Oceanicola is capable of degrading polycyclic aromatic hydrocarbons (Yuan et al, 2009;Gutierrez et al, 2017). Robiginitalea and Rubritalea have been characterized as the producers of carotenoids (Song et al, 2018) with antioxidant activities (Shindo and Misawa, 2014). In contrast, OA caused a decrease in the relative abundance of Candidatus bacilloplasma.…”

Characterization of sponge‐associated Verrucomicrobia: microcompartment‐based sugar utilization and enhanced toxin–antitoxin modules as features of host‐associated Opitutales

“…Verrucomicrobia were first proposed as a new bacterial division (Hedlund et al ., 1997) and later ranked as a separate phylum (Garrity and Holt, 2001) within the Planctomycetes ‐ Verrucomicrobia ‐ Chlamydiae superphylum. They are ubiquitous in freshwater (Zwart et al ., 2003; Arnds et al ., 2010; Chiang et al ., 2018) and have been found in various environments including soils (Janssen, 2006; Bergmann et al ., 2011), oceans (Kasai et al ., 2007; Yoon et al ., 2007b; Yoon et al ., 2007c; Yoon et al ., 2008; Song et al ., 2018; Sichert et al ., 2020) and the gastrointestinal tracts of animals (Isanapong et al ., 2013; Sato et al ., 2014), including humans (Derrien et al ., 2004; Obregon‐Tito et al ., 2015).…”