RuBisCo activase—a catalytic chaperone involved in modulating the RuBisCo activity and heat stress-tolerance in wheat

Kumar, Ranjeet; Goswami, Suneha; Dubey, Kavita; Singh, Khushboo; Singh, Jaswant; Kumar, Ashok; Kumar, Girjesh; Singh, S. D.; Bakshi, Suman; Singh, Bhupinder; Pathak, Himanshu; Chinnusamy, Viswanathan; D., Raj; Praveen, Shelly

doi:10.1007/s13562-018-0463-9

Cited by 23 publications

(18 citation statements)

References 48 publications

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“…The manner in which the mutant manifest its physiological response to water deficit and re-watering, is consistent with the results reported in maize inbred and hybrid lines ( Chen et al., 2016 ) and two wheat cultivars ( Abid et al., 2018 ), that showed a decrease in chlorophyll fluorescence and stomatal conductance during water deficit stress, but rapid “recovery” in chlorophyll fluorescence and stomatal conductance to normal levels after re-watering. This suggests that the stomatal aperture increased with re-watering thereby facilitating diffusion of CO2 from the atmosphere to the carboxylation site of RuBisCO ( Galmés et al., 2011 ; Kumar et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

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Wheat Line “RYNO3936” Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery

et al. 2020

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Random mutagenesis was applied to produce a new wheat mutant (RYNO3926) with superior characteristics regarding tolerance to water deficit stress induced at late booting stage. The mutant also displays rapid recovery from water stress conditions. Under water stress conditions mutant plants reached maturity faster and produced more seeds than its wild type wheat progenitor. Wild-type Tugela DN plants died within 7 days after induction of water stress induced at late booting stage, while mutant plants survived by maintaining a higher relative moisture content (RMC), increased total chlorophyll, and a higher photosynthesis rate and stomatal conductance. Analysis of the proteome of mutant plants revealed that they better regulate post-translational modification (SUMOylation) and have increased expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) proteins. Mutant plants also expressed unique proteins associated with dehydration tolerance including abscisic stress-ripening protein, cold induced protein, cold-responsive protein, dehydrin, Group 3 late embryogenesis, and a lipoprotein (LAlv9) belonging to the family of lipocalins. Overall, our results suggest that our new mutant RYNO3936 has a potential for inclusion in future breeding programs to improve drought tolerance under dryland conditions.

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Section: Discussionmentioning

confidence: 99%

“…the stomatal aperture increased with re-watering thereby facilitating diffusion of CO2 from the atmosphere to the carboxylation site of RuBisCO (Galmeś et al, 2011;Kumar et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Wheat Line “RYNO3936” Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery

et al. 2020

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“…which presumably enables photosynthesis to continue at higher temperatures, considering that the heat-labile nature of Rca is a major limitation on photosynthesis at higher temperatures for many plant species, including wheat (Feller et al, 1998;Crafts-Brandner and Salvucci, 2000;Sage et al, 2008;Perdomo et al, 2017;Kumar et al, 2019). The fact that other species such as cotton (Gossypium hirsutum) and maize (Zea mays; Sánchez de Jiménez et al, 1995;DeRidder and Salvucci, 2007) also induce specific isoforms of Rca in response to heat stress suggests convergent evolution of the induction of heatstable Rca isoforms in response to heat.…”

Section: Discussionmentioning

confidence: 99%

A Conserved Sequence from Heat-Adapted Species Improves Rubisco Activase Thermostability in Wheat

Scafaro

Bautsoens²,

Boer³

et al. 2019

Plant Physiol.

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The central enzyme of photosynthesis, Rubisco, is regulated by Rubisco activase (Rca). Photosynthesis is impaired during heat stress, and this limitation is often attributed to the heat-labile nature of Rca. We characterized gene expression and protein thermostability for the three Rca isoforms present in wheat (Triticum aestivum), namely TaRca1-b, TaRca2-a, and TaRca2-b. Furthermore, we compared wheat Rca with one of the two Rca isoforms from rice (Oryza sativa; OsRca-b) and Rca from other species adapted to warm environments. The TaRca1 gene was induced, whereas TaRca2 was suppressed by heat stress. The TaRca2 isoforms were sensitive to heat degradation, with thermal midpoints of 35°C 6 0.3°C, the temperature at which Rubisco activation velocity by Rca was halved. By contrast, TaRca1-b was more thermotolerant, with a thermal midpoint of 42°C, matching that of rice OsRca-b. Mutations of the TaRca2-b isoform based on sequence alignment of the thermostable TaRca1b from wheat, OsRca-b from rice, and a consensus sequence representing Rca from warm-adapted species enabled the identification of 11 amino acid substitutions that improved its thermostability by greater than 7°C without a reduction in catalytic velocity at a standard 25°C. Protein structure modeling and mutational analysis suggested that the thermostability of these mutational variants arises from monomeric and not oligomeric thermal stabilization. These results provide a mechanism for improving the heat stress tolerance of photosynthesis in wheat and potentially other species, which is a desirable outcome considering the likelihood that crops will face more frequent heat stress conditions over the coming decades.

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“…It also protects the nascent proteins from aggregation but it is heat labile. Therefore, a high temperature of >32 °C alters the composition for the accessibility of carbamylation [ 103 , 104 ].…”

Section: Impact Of High Temperature On Wheatmentioning

confidence: 99%

Rising Atmospheric Temperature Impact on Wheat and Thermotolerance Strategies

Khan

Ahmad

Ahmed

et al. 2020

Plants

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Temperature across the globe is increasing continuously at the rate of 0.15–0.17 °C per decade since the industrial revolution. It is influencing agricultural crop productivity. Therefore, thermotolerance strategies are needed to have sustainability in crop yield under higher temperature. However, improving thermotolerance in the crop is a challenging task for crop scientists. Therefore, this review work was conducted with the aim of providing information on the wheat response in three research areas, i.e., physiology, breeding, and advances in genetics, which could assist the researchers in improving thermotolerance. The optimum temperature for wheat growth at the heading, anthesis, and grain filling duration is 16 ± 2.3 °C, 23 ± 1.75 °C, and 26 ± 1.53 °C, respectively. The high temperature adversely influences the crop phenology, growth, and development. The pre-anthesis high temperature retards the pollen viability, seed formation, and embryo development. The post-anthesis high temperature declines the starch granules accumulation, stem reserve carbohydrates, and translocation of photosynthates into grains. A high temperature above 40 °C inhibits the photosynthesis by damaging the photosystem-II, electron transport chain, and photosystem-I. Our review work highlighted that genotypes which can maintain a higher accumulation of proline, glycine betaine, expression of heat shock proteins, stay green and antioxidant enzymes activity viz., catalase, peroxidase, super oxide dismutase, and glutathione reductase can tolerate high temperature efficiently through sustaining cellular physiology. Similarly, the pre-anthesis acclimation with heat treatment, inorganic fertilizer such as nitrogen, potassium nitrate and potassium chloride, mulches with rice husk, early sowing, presoaking of a 6.6 mM solution of thiourea, foliar application of 50 ppm dithiothreitol, 10 mg per kg of silicon at heading and zinc ameliorate the crop against the high temperature. Finally, it has been suggested that modern genomics and omics techniques should be used to develop thermotolerance in wheat.

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RuBisCo activase—a catalytic chaperone involved in modulating the RuBisCo activity and heat stress-tolerance in wheat

Cited by 23 publications

References 48 publications

Wheat Line “RYNO3936” Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery

Wheat Line “RYNO3936” Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery

A Conserved Sequence from Heat-Adapted Species Improves Rubisco Activase Thermostability in Wheat

Rising Atmospheric Temperature Impact on Wheat and Thermotolerance Strategies

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