Wheat Line “RYNO3936” Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery

Roux, Marlon-Schylor L. le; Burger, N. Francois V.; Vlok, Maré; Kunert, K.; Cullis, Christopher A.; Botha, Anna‐Maria

doi:10.3389/fpls.2020.01053

Cited by 14 publications

(22 citation statements)

References 161 publications

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“…Single cross-reacting peptides for both the large (LSU) and small (SSU) subunits were identified with sizes of 56 ± 4 kDa and 15 ± 2 kDa respectively. This corresponds to the sizes previously reported for wheat RuBisCO ( Figure 5 ) [ 37 , 65 ].…”

Section: Resultssupporting

confidence: 90%

“…In a previous study, the preferential degradation of the LSU in another drought-tolerant mutant line, RYNO3936, was found. These RYNO3936 mutants also expressed a high recovery capacity after senescence [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…Western blot analyses were conducted using a Bio-Rad Trans-Blot ® SD semi-dry transfer cell apparatus and polyvinylidene difluoride membranes (Hybond-P; Amersham Biosciences Ltd, Buckinghamshire, UK.) [ 37 ]. The membranes were blocked with 3% bovine serum albumin (BSA) and probed with polyclonal large (RbcL) and small (RbcS) RuBisCO Subunit IgG (RbcL and RbcS, 1:7000 [ 112 ]) and human anti-SUMO1 monoclonal antibody (1:10,000) (UBPBio, Aurora, CO, USA) diluted in buffered saline containing 3% BSA.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, our research group developed a mutant (RYNO3936) using chemically induced mutagenesis by exposing the seed of a red hard winter wheat cultivar, Tugela DN, to an alkylating mutagen known as sodium azide. This mutant outperformed the control group but also displayed rapid recovery from water deficit conditions [ 37 ]. The ability of crops to endure various degrees of drought stress is the most sought after characteristic in many breeding programs.…”

Section: Introductionmentioning

confidence: 99%

“…SUMOylation is, however, a reversible process mediated by a cluster of SUMO proteases, also referred to as cysteine proteases (CPs) [ 61 ]. Recent studies conducted on wheat and rice successfully demonstrated the function of such CPs in both plant development and response to water deficit stress [ 37 , 61 , 62 , 63 , 64 ]. The present research aimed to study the effects of water deficit stress caused by drought conditions on an ethyl methanesulfonate (EMS)-induced mutant wheat line (BIG8-1), which has enhanced tolerance to water deficit in comparison to the near-isogenic parental line (WT) BIG8.…”

Section: Introductionmentioning

confidence: 99%

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EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line

Roux

Burger

Vlok

et al. 2021

IJMS

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Drought response in wheat is considered a highly complex process, since it is a multigenic trait; nevertheless, breeding programs are continuously searching for new wheat varieties with characteristics for drought tolerance. In a previous study, we demonstrated the effectiveness of a mutant known as RYNO3936 that could survive 14 days without water. In this study, we reveal another mutant known as BIG8-1 that can endure severe water deficit stress (21 days without water) with superior drought response characteristics. Phenotypically, the mutant plants had broader leaves, including a densely packed fibrous root architecture that was not visible in the WT parent plants. During mild (day 7) drought stress, the mutant could maintain its relative water content, chlorophyll content, maximum quantum yield of PSII (Fv/Fm) and stomatal conductance, with no phenotypic symptoms such as wilting or senescence despite a decrease in soil moisture content. It was only during moderate (day 14) and severe (day 21) water deficit stress that a decline in those variables was evident. Furthermore, the mutant plants also displayed a unique preservation of metabolic activity, which was confirmed by assessing the accumulation of free amino acids and increase of antioxidative enzymes (peroxidases and glutathione S-transferase). Proteome reshuffling was also observed, allowing slow degradation of essential proteins such as RuBisCO during water deficit stress. The LC-MS/MS data revealed a high abundance of proteins involved in energy and photosynthesis under well-watered conditions, particularly Serpin-Z2A and Z2B, SGT1 and Calnexin-like protein. However, after 21 days of water stress, the mutants expressed ABC transporter permeases and xylanase inhibitor protein, which are involved in the transport of amino acids and protecting cells, respectively. This study characterizes a new mutant BIG8-1 with drought-tolerant characteristics suited for breeding programs.

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Section: Resultssupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line

Roux

Burger

Vlok

et al. 2021

IJMS

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Mutation Breeding for Adaptation to Climate Change in Seed Propagated Crops

Manjaya,

Gupta

2023

Advanced Crop Improvement, Volume 2

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Machine learning reveals complex effects of climatic means and weather extremes on wheat yields during different plant developmental stages

et al. 2021

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Rising weather volatility poses a growing challenge to crop yields in many global breadbaskets. However, empirical evidence regarding the effects of extreme weather conditions on crop yields remains incomplete. We examine the contribution of climate and weather to winter wheat yields in Ukraine, a leading crop exporter with some of the highest yield variabilities observed globally. We used machine learning to link daily climatic data with annual winter wheat yields from 1985 to 2018. We differentiated the impacts of long-term climatic conditions (e.g., temperature) and weather extremes (e.g., heat waves) on yields during the distinct developmental stages of winter wheat. Our results suggest that climatic and weather variables alone explained 54% of the wheat yield variability at the country level. Heat waves, tropical night waves, frost, and drought conditions, particularly during the reproductive and grain filling phase, constitute key factors that compromised wheat yields in Ukraine. Assessing the impacts of weather extremes on crop yields is urgent to inform strategies that help cushion farmers against growing production risks because these extremes will likely become more frequent and intense with climate change.

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Wheat Line “RYNO3936” Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery

Cited by 14 publications

References 161 publications

EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line

EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line

Mutation Breeding for Adaptation to Climate Change in Seed Propagated Crops

Machine learning reveals complex effects of climatic means and weather extremes on wheat yields during different plant developmental stages

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