Rubella serology: a comparison of four methods for exclusion of non-specific serum inhibitors

Abstract

SUMMARYThe ability of the pyrogenic silica Aerosil 380R to exclude non-specific serum inhibitors (NSI) of rubella virus haemagglutination was evaluated. The developed procedure was compared with the kaolin, heparin/AMnCl2 and dextran sulphate/ CaCl2 methods.Aerosil and kaolin were found superior for the elimination of non-specific inhibitors and high density lipoproteins (HDL). The other methods left NSI and HDL in a majority of the sera, occasionally in high titres. Aerosil seemed to be somewhat more efficient than kaolin in NSI and HDL exclusion. The Aerosil method offers the opportunity to detect sera with rubella antibody titres < 10.Among eight such sera, six were shown to contain ruibella antibodies, while two were false positives.

Imunidade à rubéola: inquérito soro-epidemiológico em hospital, Estado de São Paulo - Brasil

“…Encontrou-se uma redução de apenas 7% para IgG, fato também observado por outros autores 46 , e de 65% para IgM. O método que usa o caulim foi considerado adequado para proceder este inquérito sorológico, uma vez que pouco interferiu nos teores de IgG dos soros.…”

“…Estudos realizados com estudantes de medicina, de enfermagem e com funcionários, em diferentes hospitais, têm encontrado percentagens de 22% a 36% quanto a suscetíveis para rubéo-la 13,29,34,46 . Na Figura encontra-se a distribuição dos títulos de anticorpos para rubéola nas 1.886 funcionárias onde os mais freqüentes são 160 e 320.…”

Detection of rubella virus immunoglobulin G (IgG) and IgM antibodies in whole blood on Whatman paper: comparison with detection in sera

“…In this study, we used kaolin as the elutor and nonspecific inhibitor absorbent. Kaolin is inexpensive efficiently eliminates nonspecific inhibitors and high-density lipoproteins (27). In order to achieve an equivalent HI titer on serum and dried blood on Whatman filter paper, the amount of kaolin required to remove nonspecific inhibitors in 0.05 ml of serum in dried blood was higher than that used for 0.1 ml of serum by the standard conventional method (see Materials and Methods).…”