RT-PCR detection of breast cancer cells in sentinel lymph modes.

Kataoka, Akemi; Mori, Masaki; Sadanaga, Noriaki; Ueo, Hiroki; Tsuji, K.; Rai, Yoshiaki; Barnard, Graham F.; Sugimachi, Keizō

doi:10.3892/ijo.16.6.1147

Cited by 29 publications

(34 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, a tumour-free sentinel lymph node implies the absence of lymph node metastases in the entire lymphatic basin. This sentinel node concept had been previously confirmed by H&E examinations, immunohistochemical study and nonquantitative RT -PCR assays (Giuliano et al, 1994;Albertini et al, 1996;Veronesi et al, 1997;Krag et al, 1998;Kataoka et al, 2000;Manzotti et al, 2001;Branagan et al, 2002;Ishida et al, 2002).…”

mentioning

confidence: 61%

“…Recently, RT -PCR assays with tumour-specific gene markers such as CK19, CEA, MUC1, PIP, Mapsin and mammaglobin B have been widely used to detect occult metastasis in cancer patients (Noguchi et al, 1994(Noguchi et al, , 1996Schoenheld et al, 1994;Mori et al, 1995Mori et al, , 1998Kataoka et al, 2000;Masuda et al, 2000;Manzotti et al, 2001;Mitas et al, 2001). To date, however, no marker that is expressed in all breast cancer tissues, but not in normal lymph node has been available, partly because of the heterogeneity of marker gene expression in cancer cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Quantitative evaluation of metastases in axillary lymph nodes of breast cancer

et al. 2003

View full text Add to dashboard Cite

We have established a highly sensitive and quantitative reverse transcriptase -polymerase chain reaction (RT -PCR) method to detect axillary lymph node metastases of breast cancer. Amplifying cytokeratin 19 (CK19) mRNA transcripts using real-time TaqMan PCR made it possible to quantify axillary metastatic burden. Metastases in 358 axillary lymph nodes obtained from 23 breast cancers of 22 patients were investigated by conventional haematoxylin and eosin (H&E) staining, immunohistochemical staining and quantitative RT -PCR assay. The detection rates of axillary lymph node metastasis using H&E staining, immunohistochemistry and RT -PCR assay were 4.5, 5.9 and 13.1%, respectively. RT -PCR assay was the most sensitive of these three methods for detecting lymph node metastases. Cytokeratin 19 mRNA expression values of both histologically and immunohistochemically positive lymph nodes were significantly higher than the values for lymph nodes judged to be negative by both histological and immunohistochemical methods (Po0.0001), and those of histologically negative, but immunohistochemically positive lymph nodes were significantly higher than the values for lymph nodes judged to be negative by both histological and immunohistochemical methods (Po0.0001). Furthermore, metastatic rates of sentinel nodes were higher than the rates of nonsentinel lymph nodes as measured by all three methods. These results indicate that quantitative RT -PCR assay is a sensitive and reliable method for detecting lymph node metastasis. Furthermore, quantification of metastases in sentinel lymph nodes by quantitative RT -PCR assay may be useful to assess the entire axillary burden of breast cancer patients.

show abstract

mentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Quantitative evaluation of metastases in axillary lymph nodes of breast cancer

et al. 2003

View full text Add to dashboard Cite

show abstract

“…To our knowledge the results of five studies on RT-PCR examination performed on SLNs of breast cancer patients have been published to date (34)(35)(36)(37)(38). In four of these studies multimarker assays were used (35)(36)(37)(38).…”

Section: Discussionmentioning

confidence: 99%

“…RT-PCR has proved to be a highly sensitive method for detecting cancer cells in the regional lymph nodes or SLNs of breast cancer patients (15,16,(34)(35)(36)(37)(38). Since no ideal breast cancer marker has been identified yet, it can be hypothesized that a multimarker RT-PCR panel would increase the sensitivity and specificity of this assay (35)(36)(37).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemistry and Reverse Transcriptase Polymerase Chain Reaction on Sentinel Lymph Nodes can Improve the Accuracy of Nodal Staging in Breast Cancer Patients

et al. 2001

View full text Add to dashboard Cite

In this study the nodal staging sensitivity of sentinel lymph node biopsy (SLNB) with detailed pathological and molecular biological examination has been investigated and compared to that of axillary lymph node dissection (ALND) with routine histological evaluation. Sentinel lymph nodes (SLNs) were removed by the dual-agent injection technique in 68 patients with primary, clinically node-negative breast cancer. Forty-seven patients had negative SLNs according to hematoxylin and eosin (H&E) staining. These H&E-negative SLNs were serially sectioned and examined at 250 µm levels by anticytokeratin immunohistochemistry (IHC). In 14 patients the SLNs were also investigated by cytokeratin 20 (CK20) reverse transcriptase polymerase chain reaction (RT-PCR). SLNB with IHC increased the node-positive rate by 26% (by 40% in tumors less than or equal to 2 cm in size (pT1) and by 9% in tumors more than 2 cm but less than or equal to 5 cm in size (

show abstract

Reverse transcription-polymerase chain reaction assay for multiple mRNA markers in the detection of breast cancer metastases in sentinel lymph nodes

et al. 2001

View full text Add to dashboard Cite

The identification of specific tumor mRNA markers by reverse transcription‐polymerase chain reaction might be a valuable diagnostic adjunct for the detection of breast cancer metastases in axillary sentinel lymph nodes (SLNs). In this study we have compared the diagnostic accuracy of an extensive histopathologic examination of 146 SLNs from 123 breast carcinoma patients with that of the evaluation of 5 mRNA markers. When analyzed individually, none of the different markers attained a sensitivity higher than 77.8%, and the general concordance with the histopathologic findings ranged from 78.8 to 83.6%. In a multiple‐marker assay, taking into account the expression of at least 1 of the 5 tumor markers, the sensitivity of the test rose to 95.6%, with a specificity of 66.3% and a general concordance with the histopathologic status of 75.3%. Finally, when at least 2 of 3 markers (maspin, cytokeratin 19 and mammaglobin 1) were expressed, the concordance with either SLN or axillary lymph node status was highest (88.4% and 84.6%, respectively). The high prevalence of positive reverse transcription‐polymerase chain reaction assays in histologically uninvolved SLNs, however, may hamper extensive application of these techniques in the clinical setting. © 2001 Wiley‐Liss, Inc.

show abstract

RT-PCR detection of breast cancer cells in sentinel lymph modes.

Cited by 29 publications

References 0 publications

Quantitative evaluation of metastases in axillary lymph nodes of breast cancer

Quantitative evaluation of metastases in axillary lymph nodes of breast cancer

Immunohistochemistry and Reverse Transcriptase Polymerase Chain Reaction on Sentinel Lymph Nodes can Improve the Accuracy of Nodal Staging in Breast Cancer Patients

Reverse transcription-polymerase chain reaction assay for multiple mRNA markers in the detection of breast cancer metastases in sentinel lymph nodes

Contact Info

Product

Resources

About