RT-PCR-based analysis of microRNA (miR-1 and -124) expression in mouse CNS

Mishima, Taketoshi; Mizuguchi, Yoshiaki; Kawahigashi, Yutaka; Takami, Takeshi; Tajima, Toshihiro

doi:10.1016/j.brainres.2006.11.035

Cited by 123 publications

(85 citation statements)

References 15 publications

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“…Several key observations prompted us to examine the antitumorigenic function of miR-1 in other tissues, such as that of the lung, which expresses miR-1, albeit at a lower level than in the muscle (28). First, comparison of genome positions of mouse tumor susceptibility loci with those of mouse miRNA genes revealed that the flanking region of miR-1-1 has six substitutions in the mouse strain susceptible to lung cancer compared with the resistant strain (29).…”

mentioning

confidence: 99%

Down-regulation of Micro-RNA-1 (miR-1) in Lung Cancer: Suppression of Tumorigenic Property of Lung Cancer Cells and Their Sensitization to Doxorubicin-Induced Apoptosis by miR-1

Nasser

Datta

Nuovo

et al. 2008

Journal of Biological Chemistry

324

139

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Lung cancer is the leading cause of cancer-related deaths both in men and women in the United States, with an incidence of ϳ213,000 new cases/year (1). Approximately 80% of lung cancers are classified histopathologically as non-small cell lung cancers. At early stages of non-small cell lung cancer, the only treatment is surgery, with a 5-year overall survival rate of 40% (2), whereas chemotherapy is mostly employed for small cell lung cancer. The majority of patients have developed an aggressive form of the disease by the time of diagnosis, limiting the scope for therapeutic intervention. At this stage, several genetic and epigenetic changes take place, resulting in epithelial cell hyperplasia and, eventually, dysplasia. These changes are attributed to silencing of tumor suppressor genes, dysregulation of proto-oncogenes, and an up-regulation of genes that promote cell growth and transformation and ultimately tumor development (3).

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mentioning

confidence: 99%

Down-regulation of Micro-RNA-1 (miR-1) in Lung Cancer: Suppression of Tumorigenic Property of Lung Cancer Cells and Their Sensitization to Doxorubicin-Induced Apoptosis by miR-1

Nasser

Datta

Nuovo

et al. 2008

Journal of Biological Chemistry

324

139

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“…Reductions in endogenous miRNA levels of HepG2 cells transfected with 2 mol/L ASO miR-17-92 (ϳ300 nmol/L/ individual member) and 250 nmol/L ASO against miR-21 were determined by qRT-PCR at 48 hours and 5 days post-transfection. 30 At 48 hours, we observed an average decrease of Ϫ2.9 (⌬⌬Ct) of miR-17.5p, 20 and 92 expression as compared to non-treated cells ( Figure 5). Cells treated with ASO against miR-21 showed a Ϫ3.6 (⌬⌬Ct) decrease in expression at 48 hours post-transfection (Figure 5).…”

Section: Antisense Oligonucleotide In Vitro Knockdown Model Of the MImentioning

confidence: 82%

Elevated Expression of the miR-17–92 Polycistron and miR-21 in Hepadnavirus-Associated Hepatocellular Carcinoma Contributes to the Malignant Phenotype

Connolly

Melegari

Landgraf

et al. 2008

The American Journal of Pathology

233

180

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Alterations in microRNA (miRNA) expression in both human and animal models have been linked to many forms of cancer. Such miRNAs, which act directly as repressors of gene expression, have been found to frequently reside in fragile sites and genomic regions associated with cancer. This study describes a miRNA signature for human primary hepatitis B virus-positive human hepatocellular carcinoma. Moreover, two known oncomiRs-miRNAs with known roles in cancer-the miR-17-92 polycistron and miR-21, exhibited increased expression in 100% of primary human and woodchuck hepatocellular carcinomas surveyed. To determine the importance of these miRNAs in tumorigenesis, an in vitro antisense oligonucleotide knockdown model was evaluated for its ability to reverse the malignant phenotype. Both in human and woodchuck HCC cell lines, separate treatments with antisense oligonucleotides specific for either the miR-17-92 polycistron (all six members) or miR-21 caused a 50% reduction in both hepatocyte proliferation and anchorage-independent growth. The combination of assays presented here supports a role for these miRNAs in the maintenance of the malignant transformation of hepatocytes.

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“…MiR-124 is expressed in the central nervous system (Mishima et al, 2007) and in inner ear vestibular ganglion cells (Weston et al, 2006). Cells positive for miR-124 were present in transplanted ears (data not shown).…”

Section: Transplanted Ears Develop Hair Cells and Gangliamentioning

confidence: 99%

Transplantation of Xenopus laevis ears reveals the ability to form afferent and efferent connections with the spinal cord

Elliott

Fritzsch²

2010

Int. J. Dev. Biol.

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RT-PCR-based analysis of microRNA (miR-1 and -124) expression in mouse CNS

Cited by 123 publications

References 15 publications

Down-regulation of Micro-RNA-1 (miR-1) in Lung Cancer: Suppression of Tumorigenic Property of Lung Cancer Cells and Their Sensitization to Doxorubicin-Induced Apoptosis by miR-1

Down-regulation of Micro-RNA-1 (miR-1) in Lung Cancer: Suppression of Tumorigenic Property of Lung Cancer Cells and Their Sensitization to Doxorubicin-Induced Apoptosis by miR-1

Elevated Expression of the miR-17–92 Polycistron and miR-21 in Hepadnavirus-Associated Hepatocellular Carcinoma Contributes to the Malignant Phenotype

Transplantation of Xenopus laevis ears reveals the ability to form afferent and efferent connections with the spinal cord

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