RS 33 Bacterial penetration preceded by pH changes in in vitro leakage studies

Moshonov, Joshua; Ørstavik, Dag; Crawford, John; Shuping, G.

doi:10.1016/s0099-2399(06)80602-3

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Their finding showed that microleakage of the small molecules (butyric acid or methylene blue dye) could not be prevented, whereas leakage of bacteria-sized particles (latex beads) and large-sized protein molecules (endotoxin) could be prevented only when both sealer and vertical pressure were used in obturation techniques. More recently, Moshonov et al (1995) used S. epidermidis to detect the leakage of three root canal sealers: AH26, Roth 801 and Ketac-Endo by monitoring the turbidity of the broth and colour change of a phenol red dye in the lower chamber. They found that colour change occurred significantly earlier than the turbidity ofthe broth and the Roth 801 group allowed bacterial penetration significantly less than the other two groups (P<0.05).…”

Section: Discussion ;mentioning

confidence: 99%

An in vitro study of the coronal leakage of two root canal sealers using an obligate anaerobe microbial marker

et al. 1996

View full text Add to dashboard Cite

The aim of this in vitro study was to investigate the coronal leakage of obligate anaerobes into root canals obturated with lateral condensation of cold gutta-percha with two root canal sealers. Sixty extracted human teeth with straight, single root canals were prepared using the modified double-flared technique with balanced force under copious irrigation until the master apical file was size 40. The teeth were divided randomly into experimental groups (40 teeth) and control groups (20 teeth). In the experimental groups, 20 teeth were obturated with lateral condensation of cold gutta-percha and AH26 sealer and 20 teeth were obturated with the same technique using TubliSeal EWT sealer. In the control groups, 10 teeth were obturated with the same technique either with AH26 or TubliSeal EWT sealer. These teeth were completely sealed to serve as negative controls. The remaining 10 teeth were not obturated and served as positive controls. The root surface of each tooth was sealed with nail varnish except the apical 2 mm. The coronal part of each root canal was sealed with the cut end of polypropylene tube and placed in a glass bottle containing sterile Fastidious Anaerobe Broth (FAB). Aliquots of 0.5 mL of FAB were injected into the polypropylene tube and the model system was centrifuged at 168 g. An inoculum of Fusobacterium nucleatum in FAB was placed in each coronal chamber at 7-day intervals and daily observations were made for bacterial growth in the apical reservoir for 12 weeks. All positive control teeth showed bacterial leakage within a week, while the negative control teeth remained uncontaminated throughout the test period. All the experimental teeth exhibited leakage of bacterial metabolites within 12 weeks, ranging from 1 to 12 weeks. The mean time for complete leakage in the AH26 and the TubliSeal EWT groups was 8.4 and 8.2 weeks respectively. There was no statistically significant difference (P > 0.05) in leakage between the AH26 and the TubliSeal EWT groups.

show abstract

Section: Discussion ;mentioning

confidence: 99%