An in vitro study of the coronal leakage of two root canal sealers using an obligate anaerobe microbial marker

Abstract

The aim of this in vitro study was to investigate the coronal leakage of obligate anaerobes into root canals obturated with lateral condensation of cold gutta-percha with two root canal sealers. Sixty extracted human teeth with straight, single root canals were prepared using the modified double-flared technique with balanced force under copious irrigation until the master apical file was size 40. The teeth were divided randomly into experimental groups (40 teeth) and control groups (20 teeth). In the experimental groups, 20 teeth were obturated with lateral condensation of cold gutta-percha and AH26 sealer and 20 teeth were obturated with the same technique using TubliSeal EWT sealer. In the control groups, 10 teeth were obturated with the same technique either with AH26 or TubliSeal EWT sealer. These teeth were completely sealed to serve as negative controls. The remaining 10 teeth were not obturated and served as positive controls. The root surface of each tooth was sealed with nail varnish except the apical 2 mm. The coronal part of each root canal was sealed with the cut end of polypropylene tube and placed in a glass bottle containing sterile Fastidious Anaerobe Broth (FAB). Aliquots of 0.5 mL of FAB were injected into the polypropylene tube and the model system was centrifuged at 168 g. An inoculum of Fusobacterium nucleatum in FAB was placed in each coronal chamber at 7-day intervals and daily observations were made for bacterial growth in the apical reservoir for 12 weeks. All positive control teeth showed bacterial leakage within a week, while the negative control teeth remained uncontaminated throughout the test period. All the experimental teeth exhibited leakage of bacterial metabolites within 12 weeks, ranging from 1 to 12 weeks. The mean time for complete leakage in the AH26 and the TubliSeal EWT groups was 8.4 and 8.2 weeks respectively. There was no statistically significant difference (P > 0.05) in leakage between the AH26 and the TubliSeal EWT groups.

Sealing Capacity of Bulk-fill Resin in Endodontically Treated Teeth

“…Whereas, Khayat et al 15 reported that the contamination of DTE exposed to saliva occurred in fewer than 30 days, a result that was partly similar to that obtained in the TEF group, which showed leakage in 50% of its samples in this time interval. The short period of contamination of this same group agreed with the data of the study of Chailertvanitkul et al 16 in which the time necessary for leakage with E. faecalis to occur ranged from 7 to 86 days. Bacterial tracers have been wisely used to provide data that are biologically and clinically more significant and relevant when compared with other methods.…”

Prevalence of Apical Periodontitis in Root Canal–Treated Teeth From an Urban French Population: Influence of the Quality of Root Canal Fillings and Coronal Restorations

“…In vitro studies have revealed that even with adequate root canal fillings and regardless of the obturation technique or filling material, leakage of bacteria (from saliva or in pure cultures) and bacterial products occurs along the root canal filling, even after a short-term challenge (12)(13)(14)(15)(16)(17)(18)(19). Findings from a cross-sectional study of 1010 root canal-treated teeth suggested that the quality of the coronal restoration had a greater impact on the periradicular status than the quality of the endodontic treatment (20).…”

Avaliação ex vivo do selamento apical marginal do mta Fillapex®

“…In 1996, Chailertvanitkul et al [18] investigated the coronal leakage of obligate anaerobes using Fusobacterium nucleatum. In 1997, Chailertvanitkul et al [11] investigated microbial leakage in obturated canals by storing the specimens in artificial saliva and using Streptococcus sanguinis and Prevotella intermedia to test for leakage.…”