2000
DOI: 10.1017/s1355838200992288
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Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2

Abstract: Chemical modifications and processing of the 18S, 5.8S, and 25S ribosomal RNAs from the 35S pre-ribosomal RNA depend on an important set of small nucleolar ribonucleoprotein particles (snoRNPs). Genetic depletion of yeast Gar1p, an essential common component of H/ACA snoRNPs, leads to inhibition of uridine isomerizations to pseudouridines on the 35S pre-rRNA and of the early pre-rRNA cleavages at sites A1 and A2, resulting in a loss of mature 18S rRNA synthesis. To identify Gar1p functional partners, we screen… Show more

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Cited by 44 publications
(49 citation statements)
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“…Y0341-npa1::GFP cells were treated as previously described (10). Detection of Npa1p-ZZ by immunoelectron microscopy was performed as described by Henras et al (38).…”
Section: Methodsmentioning
confidence: 99%
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“…Y0341-npa1::GFP cells were treated as previously described (10). Detection of Npa1p-ZZ by immunoelectron microscopy was performed as described by Henras et al (38).…”
Section: Methodsmentioning
confidence: 99%
“…A total of 500 l of extract corresponding to 5 mg of proteins was loaded on a 10 to 30% glycerol gradient. Preparation of the gradient, loading of the extract, centrifugation, and collection of fractions were performed as described previously (10).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…This may be due to the transient nature of these interactions or concurrent disassembly of the SSU processome when Bud23 enters the particle. The behavior of Bud23 is reminiscent of another putative methyltransferase, Rrp8, that shows synthetic lethality with gar1 mutants and strongly affects cleavage at A2 but does not immunoprecipitate SSU processome components or H/ACA or C/D box snoRNPs (Bousquet-Antonelli et al 2000). However, how Rrp8 mediates its role in rRNA processing or its putative substrate is not understood.…”
Section: Bud23 Work At the Transition Of Early To Middle Stage Of 40mentioning
confidence: 99%
“…Yeast Gar1p, Nhp2p, Nop10p, Nop1p, ZZ-tagged proteins, and hNop10 were detected as described in Bousquet-Antonelli et al (2000), Henras et al (2001), and Dez et al (2002). Yeast Cbf5p was detected using purified antibodies, diluted 500-fold, raised in rabbits (by Eurogentec) against the recombinant protein lacking the C-terminal KKE repeats.…”
Section: Western Analysismentioning
confidence: 99%