RrGT1, a key gene associated with anthocyanin biosynthesis, was isolated from Rosa rugosa and identified via overexpression and VIGS

Sui, Xiaoming; Zhao, Mingyuan; Han, Xu; Zhao, Ling; Xu, Zongda

doi:10.1016/j.plaphy.2018.11.022

Cited by 8 publications

(7 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This causes the nanopores between the adjacent cell wall layers to expand, manifesting as volume swelling at the macroscale [23]. Meanwhile, water adsorption causes the moisture content inside the cell wall to increase, which is beneficial for the survival of decay fungi due to sufficient moisture supply, thus encouraging the degradation of wood [19,[24][25][26]. Consequently, wood deformation and degradation are closely related to cell wall moisture content; the water uptake of wood originates from the dynamic nanopores and the hydroxyl groups of the cell wall components.…”

Section: Introductionmentioning

confidence: 99%

Cell Wall Bulking by Maleic Anhydride for Wood Durability Improvement

et al. 2020

Forests

View full text Add to dashboard Cite

Wood is susceptible to swelling deformation and decay fungi due to moisture adsorption that originates from the dynamic nanopores of the cell wall and the abundant hydroxyl groups in wood components. This study employed as a modifier maleic anhydride (MAn), with the help of acetone as solvent, to diffuse into the wood cell wall, bulk nanopores, and further chemically bond to the hydroxyl groups of wood components, reducing the numbers of free hydroxyl groups and weakening the diffusion of water molecules into the wood cell wall. The derived MAn-bulked wood, compared to the control wood, presented a reduction in water absorptivity (RWA) of ~23% as well as an anti-swelling efficiency (ASE) of ~39% after immersion in water for 228 h, and showed an improvement in decay resistance of 81.42% against white-rot fungus and 69.79% against brown-rot fungus, respectively. The method of combined cell wall bulking and hydroxyl group bonding could effectively improve the dimensional stability and decay resistance with lower doses of modifier, providing a new strategy for wood durability improvement.

show abstract

Section: Introductionmentioning

confidence: 99%

Cell Wall Bulking by Maleic Anhydride for Wood Durability Improvement

et al. 2020

Forests

View full text Add to dashboard Cite

show abstract

“…In the present study, proteomic data identified some transferases in black rice, including proteins with putative methyltransferase, acyltransferase, and glycosyltransferase activities. These transferases may contribute to anthocyanin modifications (Sasaki et al, 2014;Provenzano et al, 2014;Li et al, 2017b;Wang et al, 2018;Sui et al, 2019). We found some proteins related to anthocyanin synthesis with the higher expression in black rice than white rice.…”

Section: Discussionmentioning

confidence: 78%

“…Li and others (Li et al, 2017a) stated that overexpression of a GT gene implied higher anthocyanin contents in Arabidopsis thaliana. However, GT gene overexpression decreased glycosyltransferase activity and reduced color intensity in Rosa rugosa (Sui et al, 2019).…”

Section: Discussionmentioning

confidence: 95%

Black rice cultivar from Java Island of Indonesia revealed genomic, proteomic, and anthocyanin nutritional value

et al. 2021

View full text Add to dashboard Cite

Black rice is considered to be functional food containing anthocyanins as bioactive compounds. This study examined the genomic and proteomic patterns in local black rice from Java Island, Indonesia, with attention to the mechanism of anthocyanin synthesis. Three kinds of black rice from Java Island, including black rice from East Java (BREJ), black rice from Central Java (BRCJ), and black rice from West Java (BRWJ), were studied in comparison to white rice (WREJ) and red rice (RREJ). Genomic profiling was done by simple sequence repeat (SSR) analysis, and sequencing of red coleoptile (Rc) and glycosyltransferase (GT) genes, followed by in silico analysis. Total anthocyanin was investigated by ultra-high performance liquid chromatography- diode array detector (UHPLC-DAD). The proteomic profiles were determined by liquid-chromatography and mass spectrometry of tryptic peptides. The SSR profiles showed a specific band in each black rice variant. The Rc gene exon-2 sequences were similar in the three black rice cultivars. The GT gene sequence was identified as a new variant that correlates with the purple stem, leaf, bran, and whole grain morphology seen exclusively in the BRWJ cultivar. The anthocyanin composition in Java black rice is diverse. The highest cyanidin level was seen in BRWJ and the highest level of peonidin-3-O-glucoside in BREJ. Proteomic profiling of the black rice cultivars demonstrated that the expression of proteins that might be related to the levels of anthocyanin synthesis varied. These studies conclude that the genomic, proteomic and anthocyanins composition of Java black rice cultivars may be used the improvement of their functional nutrition values.

show abstract

“…All actions were done on a CFX96 Real-time System using SYBR Green Dye according to previous study (Wu et al, 2020). The expression patterns were analyzed through the 2 -DDCt method with RrGADPH as an internal control (Sui et al, 2019). All primers used in this study are listed (Supplementary Table 2).…”

Section: Qrt-pcr Analysismentioning

confidence: 99%

Transcriptome and chemical analyses revealed the mechanism of flower color formation in Rosa rugosa

Wang

Li²,

Zhu³

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Rosa rugosa is a famous Chinese traditional flower with high ornamental value and well environmental adapt ability. The cultivation of new colorful germplasms to improve monotonous flower color could promote its landscape application. However, the mechanism of flower color formation in R. rugosa remains unclear. In this study, combined analyses of the chemical and transcriptome were performed in the R. rugosa germplasms with representative flower colors. Among the identified anthocyanins, cyanidin 3,5-O-diglucoside (Cy3G5G) and peonidin 3,5-O-diglucoside (Pn3G5G) were the two dominant anthocyanins in the petals of R. rugosa. The sum content of Cy3G5G and Pn3G5G was responsible for the petal color intensity, such as pink or purple, light- or dark- red. The ratio of Cy3G5G to Pn3G5G was contributed to the petal color hue, that is, red or pink/purple. Maintaining both high relative and high absolute content of Cy3G5G may be the precondition for forming red-colored petals in R. rugosa. Cyanidin biosynthesis shunt was the dominant pathway for anthocyanin accumulation in R. rugosa, which may be the key reason for the presence of monotonous petal color in R. rugosa, mainly pink/purple. In the upstream pathway of cyanidin biosynthesis, 35 differentially expressed structural genes encoding 12 enzymes co-expressed to regulate the sum contents of Cy3G5G and Pn3G5G, and then determined the color intensity of petals. RrAOMT, involved in the downstream pathway of cyanidin biosynthesis, regulated the ratio of Cy3G5G to Pn3G5G via methylation and then determined the color hue of petals. It was worth mentioning that significantly higher delphinidin-3,5-O-diglucoside content and RrF3’5’H expression were detected from deep purple-red-flowered 8-16 germplasm with somewhat unique and visible blue hue. Three candidate key transcription factors identified by correlation analysis, RrMYB108, RrC1, and RrMYB114, might play critical roles in the control of petal color by regulating the expression of both RrAOMT and other multiple structural genes. These results provided novel insights into anthocyanin accumulation and flower coloration mechanism in R. rugosa, and the candidate key genes involved in anthocyanin biosynthesis could be valuable resources for the breeding of ornamental plants in future.

show abstract

RrGT1, a key gene associated with anthocyanin biosynthesis, was isolated from Rosa rugosa and identified via overexpression and VIGS

Cited by 8 publications

References 33 publications

Cell Wall Bulking by Maleic Anhydride for Wood Durability Improvement

Cell Wall Bulking by Maleic Anhydride for Wood Durability Improvement

Black rice cultivar from Java Island of Indonesia revealed genomic, proteomic, and anthocyanin nutritional value

Transcriptome and chemical analyses revealed the mechanism of flower color formation in Rosa rugosa

Contact Info

Product

Resources

About