RpoE Facilitates Stress-Resistance, Invasion, and Pathogenicity of Escherichia coli K1

Yu, Fan; Bai, Jing; Xi, Daoyi; Yang, Bin

doi:10.3390/microorganisms10050879

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…Pelagibacter’ HTCC7211, TMED112 CCGs have lost the two‐component system (EnvZ‐OmpR; ChvG‐ChvI; NtrY‐NtrX and RegB‐RegA) related to nitrogen regulation, acidity sensing, osmotic stress, and redox response. Regarding sigma factors, while strain HTCC7211 had two ( rpo D and rpo H), TMED112 CCGs also encode rpo E, involved in the response to several environmental stresses (Fan et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

Single‐amplified genomes reveal most streamlined free‐living marine bacteria

Roda‐Garcia

Haro-Moreno

Rodrı́guez-Valera

et al. 2023

Environmental Microbiology

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Evolutionary adaptations of prokaryotes to the environment sometimes result in genome reduction. Our knowledge of this phenomenon among free-living bacteria remains scarce. We address the dynamics and limits of genome reduction by examining one of the most abundant bacteria in the ocean, the SAR86 clade. Despite its abundance, comparative genomics has been limited by the absence of pure cultures and the poor representation in metagenome-assembled genomes. We co-assembled multiple previously available single-amplified genomes to obtain the first complete genomes from members of the four families. All families showed a convergent evolutionary trajectory with characteristic features of streamlined genomes, most pronounced in the TMED112 family. This family has a genome size of ca. 1 Mb and only 1 bp as median intergenic distance, exceeding values found in other abundant microbes such as SAR11, OM43 and Prochlorococcus. This genomic simplification led to a reduction in the biosynthesis of essential molecules, DNA repair-related genes, and the ability to sense and respond to environmental factors, which could suggest an evolutionary dependence on other co-occurring microbes for survival (Black Queen hypothesis). Therefore, these reconstructed genomes within the SAR86 clade provide new insights into the limits of genome reduction in free-living marine bacteria.

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Section: Resultsmentioning

confidence: 99%

Single‐amplified genomes reveal most streamlined free‐living marine bacteria

Roda‐Garcia

Haro-Moreno

Rodrı́guez-Valera

et al. 2023

Environmental Microbiology

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“…The phosphate-sensing PhoR-PhoB system has also been associated with E. coli virulence and is required for expression of a gene that regulates type I pili synthesis in CFT073 ( 56 ) ( 57 , 58 ). RpoE has been implicated in virulence of Salmonella and E. coli , including UPEC strains ( 59 – 62 ). RpoE is stress-induced sigma subunit for RNA polymerase that responds to high temperature and osmolarity, misfolded proteins in the outer membrane, periplasm, and inner membrane, metal ion exposure, entry into stationary phase, and elevated (p)ppGpp ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

The distinct transcriptome of virulence-associated phylogenetic group B2 Escherichia coli

Hogins,

Xuan,

Zimmern

et al. 2023

Microbiol Spectr

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Escherichia coli strains of phylogenetic group B2 are often associated with urinary tract infections (UTIs) and several other diseases. Recent genomic and transcriptomic analyses have not suggested or identified specific genes required for virulence, but have instead suggested multiple virulence strategies and complex host-pathogen interactions. Previous analyses have not compared core gene expression between phylogenetic groups or between pathogens and nonpathogens within phylogenetic groups. We compared the core gene expression of 35 strains from three phylogenetic groups that included both pathogens and nonpathogens after growth in a medium that allowed comparable growth of both types of strains. K-means clustering suggested a B2 cluster with 17 group B2 strains and two group A strains; an AD cluster with six group A strains, five group D strains and one B2 strain; and four outliers which included the highly studied model uropathogenic E. coli strains UTI89 and CFT073. Half of the core genes were differentially expressed between B2 and AD cluster strains, including transcripts of genes for all aspects of macromolecular synthesis—replication, transcription, translation, and peptidoglycan synthesis—energy metabolism, and environmental-sensing transcriptional regulators. Notably, core gene expression between nonpathogenic and uropathogenic transcriptomes within phylogenetic groups did not differ. If differences between pathogens and nonpathogens exist, then the differences do not require transcriptional reprogramming. In summary, B2 cluster strains have a distinct transcription pattern that involves hundreds of genes. We propose that this transcription pattern is one factor that contributes to virulence. IMPORTANCE Escherichia coli is a diverse species and an opportunistic pathogen that is associated with various diseases, such as urinary tract infections. When examined, phylogenetic group B2 strains are more often associated with these diseases, but the specific properties that contribute to their virulence are not known. From a comparative transcriptomic analysis, we found that group B2 strains grown in a nutrient-rich medium had a distinct transcription pattern, which is the first evidence that core gene expression differs between phylogenetic groups. Understanding the consequences of group B2 transcription pattern will provide important information on basic E. coli biology, the basis for E. coli virulence, and possibly for developing therapies for a majority of urinary tract infections and other group B2-associated diseases.

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“…RpoE directly regulates expression of some virulence genes such as ompA, cnf1, fimB, ibeA , and the capsule genes kpsM , and kpsF . Also, RpoE indirectly modulates some virulence genes such as fimA, aslA , and traJ (Fan et al 2022 ). This example shows the broad impact of SR on regulation of virulence during bacteremia.…”

Section: Bacterial Factors Mediating Adaptive Response To Bloodmentioning

confidence: 99%

Determinants of bacterial survival and proliferation in blood

Lê-Bury,

Echenique-Rivera,

Pizarro-Cerdá

et al. 2024

FEMS Microbiology Reviews

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Bloodstream infection is a major public health concern associated with high mortality and high healthcare costs worldwide. Bacteremia can trigger fatal sepsis whose prevention, diagnosis and management have been recognized as a global health priority by the World Health Organization. Additionally, infection control is increasingly threatened by antimicrobial resistance, which is the focus of global action plans in the framework of a One Health response. In-depth knowledge of the infection process is needed to develop efficient preventive and therapeutic measures. The pathogenesis of bloodstream infection is a dynamic process resulting from the invasion of the vascular system by bacteria, which finely regulate their metabolic pathways and virulence factors to overcome the blood immune defenses and proliferate. In this review, we highlight our current understanding of determinants of bacterial survival and proliferation in the bloodstream and discuss their interactions with the molecular and cellular components of blood.

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RpoE Facilitates Stress-Resistance, Invasion, and Pathogenicity of Escherichia coli K1

Cited by 5 publications

References 48 publications

Single‐amplified genomes reveal most streamlined free‐living marine bacteria

Single‐amplified genomes reveal most streamlined free‐living marine bacteria

The distinct transcriptome of virulence-associated phylogenetic group B2 Escherichia coli

Determinants of bacterial survival and proliferation in blood

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