RP-LC Determination of Rutin in Amaranthus spinosus Linn. Whole Plant Powder

Hypocotyls explants of 1-week-old Ruta gravoelens L. seedlings showed high regeneration ability on Murashige and Skoog's medium supplemented with 5.2 mM H 2 PO 4 -, 250 mM adenine sulphate, 3.0% sucrose and 8.87 lM BA. Increasing phosphate and adenine sulphate concentrations had pronounced effects on organogenesis and shoot growth compared to the stander MS medium. Multiple shoots were successfully maintained for 60 days in static liquid medium without any hyperhydricity phenomena. Uncountable shoot buds and shoots were developed on the leaf surface which was in contact with the medium. The growth index increased linearly during the culture course (R 2 = 0.992-0.999) and the highest GI (30.43) was achieved on medium enriched with 90 mM nitrogen and 4.5% sucrose. Medium supplemented with 6.0% sucrose and 60 mM N yielded the highest biomass (4.37 g per flask). However, medium with the stander N content and 6.0% sucrose developed the maximum percentage of biomass (6.67%). The highest percentage of furanocoumarins (11.46 mg g -1 dry weight) calculated as xanthotoxin was produced in medium enriched with 90 mM N and 4.5 or 6.0% sucrose, whereas the maximum rutin (73.5 mg g -1 dry weight) was on medium supplemented with 90 mM N and 4.5% sucrose. Developing system seems to be used for secondary metabolites production because the shoot rooting did not appear. So, all energy is devoted for shoots multiplication, their growth and secondary metabolites production.

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A Pharmacological and Phytochemical Evaluation of Medicinal Plants Used by the Harbang Clan of the Tripura Tribal Community of Mirsharai Area, Chittagong District, Bangladesh

Rahmatullah

Rahman

Hossan

et al. 2010

The Journal of Alternative and Complementary Medicine

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Information on phytochemicals and pharmacologic activity studies conducted on a number of the plants (used by Tripura tribal healers) by modern scientific methods validated the traditional use of a number of plants and suggested that they can form a good source of newer drugs. The survey further highlighted the importance of gathering such ethnomedicinal information for effective conservation of tribal medicinal knowledge and medicinally important plant species.

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RP-LC Determination of Rutin in Amaranthus spinosus Linn. Whole Plant Powder

Cited by 4 publications

References 6 publications

Enhanced in vitro production of Ruta graveolens L. coumarins and rutin by mannitol and ventilation

Enhanced in vitro production of Ruta graveolens L. coumarins and rutin by mannitol and ventilation

In vitro mass production of Ruta gravoelens L. for secondary products production

A Pharmacological and Phytochemical Evaluation of Medicinal Plants Used by the Harbang Clan of the Tripura Tribal Community of Mirsharai Area, Chittagong District, Bangladesh

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