Enhanced in vitro production of Ruta graveolens L. coumarins and rutin by mannitol and ventilation

Mohamed, M. A.H.; Ibrahim, Talaat A.

doi:10.1007/s11240-012-0199-5

Cited by 15 publications

(3 citation statements)

References 36 publications

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“…On the literature, there are works which have reported the quantification of rutin in Sambucus nigra and Ruta graveolens flowers that were similar to the present work, or even superior (Dawidowicz & Wianowska, 2009;Mohamed & Obrahim, 2012;Ueng et al, 2015). Differences in this study's results compared to the literature can be explained by the growing conditions of the plants, climate and botanical variety, besides the use of other parts of the plant such as the stem, which has a lower concentration of rutin.…”

Section: Extraction Using Ethanol For Commercial Purificationsupporting

confidence: 84%

Rutin in herbs and infusions: screening of new sources and consumption estimation

et al. 2020

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Rutin has shown promising results in reducing various human diseases. However, many plants consumed in Brazil do not have their rutin content reported in the literature yet. More than eighty plants were analyzed using aqueous (popularly known as tea) and ethanolic extraction (employed in commercial rutin purification) by liquid chromatography. Rutin was found in 47 samples when ethanol extraction was used. Echinodorus grandiflorus had the highest content (14,878.7 mg 1000 g-1), followed by Sambucus nigra, Drimys winteri and Taraxacum officinale. On aqueous infusion, Echinodorus grandiflorus, Sambucus nigra, Drimys winteri and Ruta graveolens presented the highest levels of rutin, ranging from 15.5 to 29.7 mg of rutin in 300 mL of infusion from 2 g of the plant. Echinodorus grandiflorus, Sambucus nigra, Drimys winteri, Taraxacum officinale and Ruta graveolens presented a high amount of flavonoid and might be good alternatives as ingredients in food and pharmaceuticals in order to obtain health benefits.

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Section: Extraction Using Ethanol For Commercial Purificationsupporting

confidence: 84%

Rutin in herbs and infusions: screening of new sources and consumption estimation

et al. 2020

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“…Moreover, root and shoot traits of plantlets acclimatized under 2-3% Suc plus different degrees of Man were sharply decreased in relation to their concentrations in the culture medium (Custódio et al 2004;Madhulatha et al 2006;Samarina et al 2020). In Ruta graveolens, the growth performance and production of coumarins and rutin in the shoot cultures were regulated by 3% Suc + 1% Man in the culture medium (Mohamed and Ibrahim 2012). Leaf and root osmotic potential of plantlets acclimatized under 3% Suc + 2.5% Man were more negative than control and 6% Suc.…”

Section: Resultsmentioning

confidence: 94%

“…It was con rmed that the physiological and morphological traits as well as curcuminoids yield of in-vitro plantlets acclimatized using iso-osmotic treatment of 3% Suc + 2.5% Man were better compared to control during long-term cultivation. In Ruta graveolens, plant morphological characters in mannitol acclimatized plantlets were unaffected, whereas coumarins and rutin in terms of content and total yield were regulated in response to increase in Man concentration in the culture medium (Mohamed and Ibrahim 2012).…”

Section: Ex-vitro Adaptationmentioning

confidence: 97%

In-Vitro Acclimatization of Curcuma Zedoaria Under Iso-Osmotic Treatments, Ex-Vitro Adaptation and Agronomic Traits in Greenhouse Conditions

Tisarum¹,

Bayaraa²,

Sotesaritkul³

et al. 2021

Preprint

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Low survival rate, poor adaptation to ex-vitro environments, and time required for hardening the plants to cope with fluctuated environments of field trial are identified as major barriers in this technology. In present study, iso-osmotic adjustment in the culture medium using sucrose and/or mannitol was applied to the in-vitro cloning of Curcuma zedoaria (white turmeric) plants, which were transferred to ex-vitro conditions and subsequently cultivated in the greenhouse conditions prior to harvest after 9 months. During both in-vitro and ex-vitro development of plant, growth and physiological traits under 3% sucrose (Suc) + 2.5% mannitol (Man) were lower than those in control (3% Suc; conventional tissue culture). Interestingly, pseudostem height and root length in acclimatized plantlets under 3% Suc + 2.5% Man were sharply dropped by 60.13% and 92.37% over control, respectively, resulting in a decrease in the ex-vitro adaptation by 56.27% and 33.33% over the control. A positive relationship between reduction of net photosynthetic rate (Pn) and sucrose concentration in the leaf tissues was evidently observed. Remarkably, the morphological and physiological traits of aboveground and underground parameters of acclimatized plantlets under 3% Suc + 2.5% Man were maximized over control, leading to high yield of curcuminoids (229.4 mg plant− 1) in the dry rhizome (31 g plant− 1) when cultivated under greenhouse microenvironments for 9 months. Based on this investigation, we propose that plantlets of C. zedoaria micropropagated using 3% Suc + 2.5% Man can readily acclimatize under ex-vitro conditions and subsequently develop as healthy plants with compact and uniform size.

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In Vitro Screening of Crop Plants for Abiotic Stress Tolerance

Maleki

Ghorbanpour

Nikabadi

et al. 2019

Recent Approaches in Omics for Plant Resilience to Climate Change

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Enhanced in vitro production of Ruta graveolens L. coumarins and rutin by mannitol and ventilation

Cited by 15 publications

References 36 publications

Rutin in herbs and infusions: screening of new sources and consumption estimation

Rutin in herbs and infusions: screening of new sources and consumption estimation

In-Vitro Acclimatization of Curcuma Zedoaria Under Iso-Osmotic Treatments, Ex-Vitro Adaptation and Agronomic Traits in Greenhouse Conditions

In Vitro Screening of Crop Plants for Abiotic Stress Tolerance

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