RP-HPLC/Pre-Column Derivatization for Analysis of Omeprazole, Tinidazole, Doxycycline and Clarithromycin

Abstract

A validated, reliable and accurate reversed-phase high performance liquid chromatographic method using pre-column derivatization was adopted for the simultaneous determination of two ternary mixtures containing omeprazole, tinidazole and doxycycline hyclate or clarithromycin. Separation was achieved on a C18 column, through a gradient elution system using acetonitrile-methanol-water adjusted to pH = 6.60. Drugs were detected at 277 nm over concentration ranges of 1-112, 5-125, 2.5-550 and 2.5-100 µg/mL for omeprazole, tinidazole, doxycycline hyclate and clarithromycin, respectively. This is the first method that has isolated and identified clarithromycin derivative by infrared and mass spectroscopy. This method is the first study for the simultaneous determination of omeprazole, tinidazole, doxycycline hyclate and clarithromycin in combined mixtures and pharmaceutical formulations.

Methods for the separation, preconcentration, and determination of tetracycline antibiotics

“…In the majority of cases, sample preparation for the determination of tet racycline antibiotics in pharmaceuticals consists in the grinding of a sample and its dissolution in twice dis tilled water [116], 0.01 M HCl [117][118][119][120], a citrate buffer solution [121], a Britton-Robinson buffer solu tion [122], or a mixture of this buffer solution (pH 2.0) with acetonitrile (70 : 30) [123]. For the intensification of sample preparation, dissolution is sometimes per formed in an ultrasonic bath [116,118,121,123,124].…”

“…Photometric UV [23,51,53,60,73,74,80,81,89,113,124,134], diode array [15,19,22,29,37,55,68,72,84,86,138,139], and fluorescence [34,58,71,83,93,94] detectors are used for the detection of tetracy cline antibiotics. The number of works regarding the determination of tetracycline antibiotics by the HPLC with a mass spectrometric detector noticeably increased [15,21,27,32,35,69,100,105,106,112,114].…”

Highly Sensitive and Selective Polyaniline Nanofiber–Based Voltammetric Sensor for the Quantification of Tinidazole

“…Polymers have been extensively studied for the development of drug sensors based on their high conductivity, fast response times, and room temperature operation. The review of literature revealed that a few methods have been explored for the detection of TNZ, which includes titrimetric, 51 TLC, 52 spectrophotometric, [53][54][55] HPLC, 56 RP-HPLC 57,58 , and UV-visible spectrophotometry. [45][46][47][48] Tinidazole (TNZ), chemically 1-(2-ethyl sulfonyl ethyl)-2-methyl-5-nitroimidozole [A] is an antiparasitic drug used against protozoan infection including giardiasis, amoebiasis, and vaginal trichomoniasis.…”

Simultaneous Determination of Omeprazole, Tinidazole and Clarithromycin in Bulk Powder and Helicure Tablets by HPLC

“…Tinidazole was estimated in body fluids and in pharmaceutical formulations by spectrophotometry [24][25][26][27][28][29], potentiometry [29], HPLC methods [29][30][31], polarography [32,33] and resonance light scattering technique [34]. Clarithromycin has been reported to be estimated in body fluids and in pharmaceutical formulations by spectrophotometry [35], HPLC methods [36][37][38][39][40][41][42][43][44]. Omeprazole, Tinidazole and Clarithromycin were simultaneously determined by spectrophotometry [45,46].…”