RP ‐ HPLC Method for Determination of Piperine from Piper longum Linn. and Piper nigrum Linn

Abstract

Piper longumLinn. andPiper nigrumLinn. are used as spices and medicines. Quantitative determination of piperine was undertaken to provide an easy and simple analytical method, which can be used as a routine quality control method. RP-HPLC was performed using methanol and water as mobile phase. The detection and quantification was performed at a wavelength of 345 nm. Linearity of detector response for piperine was between the concentrations 0.005% to 0.1%. The correlation coefficient obtained for the linearity was 0.998. The assay value of piperine for fruit and root ofP. longumwas found to be 0.879% and 0.31%. The assay value of piperine for fruit ofP. nigrumwas 4.5%. The recovery value of standard piperine was 99.4%. Low value of standard deviation and coefficient of variation are indicative of high precision of the method.

Facile and surfactant-free synthesis of Pd nanoparticles by the extract of the fruits of Piper longum and their catalytic performance for the Sonogashira coupling reaction in water under ligand- and copper-free conditions

“…The described strategy for Pd NPs is straightforward, robust, environmentally friendly, and costeffective, and shows considerable great potential. The 20 synthesized Pd NPs by this method are quite stable and can be kept under inert atmosphere for several months.…”

“…The formation of Pd NP was monitored with the help of UVvis spectrometry. The UV-vis spectrum of green synthesized Pd NPs using piper longum fruits extract ( Figure 5) showed the significant changes in the absorbance maxima due to surface 20 plasmon resonance demonstrating the formation of Pd NPs. Also, the progression of the reaction, formation and stability of palladium nanoparticles were controlled by UV-vis spectroscopy.…”

Process development studies for recovery of bio active isolates from spent black pepper generated from ayurvedic industry

“…HPLC analysis of crystallized piperine was performed by standard method (Santosh et al, 2005;Shingate et al, 2013). An analytical high performance liquid chromatography (Shimadzu, LC 20A HPLC series, Kyoto, Japan) with variable wavelength, programmable photo diode array detector, column oven and reverse phase 5 m C 18 Supelco column (250 × 4.6 mm) (Sigma-Aldrich, USA) was used.…”

Enhanced extraction of oleoresin from Piper nigrum by supercritical carbon dioxide using ethanol as a co‐solvent and its bioactivity profile

“…The TV were estimated by method reported by Shaikh et al (). The piperine content was estimated by HPLC method reported by Santosh et al () with slight modifications as follows: A Merck pheurosphere STAR RP18e column (5 µm × 250 mm × 4.6 mm) was used for analysis on Dionex HPLC system on a diode array detector (Thermo Fischer, Mumbai, India). The mobile phase consisted of methanol: deionized water (DI) (80:20, vol/vol).…”