RP HPLC Method Development for Simultaneous Estimation of Etoricoxib and Thiocolchicoside

Sharma, Anchal; Chaudhary, Amit; Singh, Bhupendra

doi:10.9734/jpri/2022/v34i6a35430

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…The consistent results observed by many authors, when the Alfvén waves interaction considered [34,67,68]. When the Alfvén waves interact nonlinearly in MHD turbulent plasma, then their spectra steeper the Kolmogorov like spectra (k À5=3 ) studied by Goldreich and Sridhar [41,59,63,64]; Goldstein et al [66]. The strong turbulent studied by Schekochihin et al [69] with slop k À7=3 and observed particle asymptotic behavior in space plasma.…”

Section: Numerical Simulation Resultssupporting

confidence: 72%

“…Figure 2(c-d) shows the formation of localized structures for Alfvén waves at t = 11, 16 and after few step the system reaches to its quasi steady state. The Alfvén waves spread there intensity peaks when the magnetic field intensity raised [63]. shows the global spatial structures, where the perturbation takes energy from the interaction of Alfvén wave and form their complex structures (Figure 2(d)).…”

Section: Numerical Simulation Resultsmentioning

confidence: 99%

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Perspective Chapter: Numerical Simulation to Study Alfvén Waves

Singh Jatav

2024

Advancements in Fine Particle Plasmas

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This chapter presents the Alfvén waves self-interaction in approximately zero-β plasma (cold plasma), which is applicable to coronal heating. We consider thermal to magnetic pressure ratio β≈0 (cold plasma). When we consider magnetic field transverse perturbation in approximately zero-β plasma, the model dynamical equation of Alfvén wave self interaction turns out to be the modified Zakharov system equation. Numerical simulation has been carried out to study the effect of Alfvén wave self interaction and transverse perturbation, which results in the formation of magnetic field profile structures (coherent structures) and power spectrum for coronal heating and particle acceleration in space plasma. The investigated results reveals that the system of magnetic field profile structures reaches to quasi-steady state and the power spectral index approaches the k−5/3, which is consistent with Kolmogorov scaling.

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Section: Numerical Simulation Resultssupporting

confidence: 72%

Section: Numerical Simulation Resultsmentioning

confidence: 99%

Perspective Chapter: Numerical Simulation to Study Alfvén Waves

Singh Jatav

2024

Advancements in Fine Particle Plasmas

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“…The standard was prepared by dissolving 1 mg/mL in HPLC grade methanol and filtered through a 0.45 μm nylon filter. The retention time of the standard was compared to the samples (Navneet Kumar et al, 2017;Pushparaj, 2017). The mobile phase used was methanol and water in a ratio of 50:50.…”

Section: Hplc Analysismentioning

confidence: 99%

Bioprospecting of serratiopeptidase-producing bacteria from different sources

Nair,

Subathra Devi

2024

Front. Microbiol.

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Anti-inflammatory enzymes have wide applications in the pharmaceutical industry. The objective of this study was to find new and efficient strains for the commercial production of serratiopeptidase enzyme. Vast number of samples were processed for the isolation of potent strains. The experimental treatment includes processing of twenty soil samples, silkworm gut, and sugarcane stem. The total protein and protease activity was estimated by Lowry’s method and casein hydrolysis. The HRBC stabilization assay was performed for finding the anti-inflammatory potential of all strains. The serratiopeptidase production was confirmed by HPLC with the standard. Molecular characterization of selected potent strains was done by 16S rDNA and confirmed the taxonomy. The one step rapid purification of serratiopeptidase was performed by Ultra three phase partitioning method. The clot lysis potential of the Serratia marcescens VS56 was observed by modified Holmstorm method. The results of the study revealed that among the 60 strains, 12 strains were protease-positive on skim milk agar plates and showed significant protease activity. All 12 strains were screened for serratiopeptidase using high-performance liquid chromatography (HPLC) and VS56, VS10, VS12 and VS18 showed a similar retention time (4.66 ± 0.10 min) with standard. The selected potent strain, Serratia marcescens VS56 showed a proteolytic activity of 21.30 units/mL and produced a total protein of 102 mg/mL. The HRBC suspension results also showed a percentage of 94.6 ± 1.00 protection, which was compared to the standard diclofenac. The clot lysis potential of Serratia marcescens VS56 was 53% in 4 h. Furthermore, the molecular weight of the protein was identified to confirm the presence of serratiopeptidase. The study hence contributed successfully to isolating, screening, and identifying a potent producer for serratiopeptidase from an environmental source. This inherent advantage of the strain will undoubtedly contribute much to the coco comm commercial production of serratiopeptidase in the near future.

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Development and validation of an analytical method for the simultaneous estimation of Etoricoxib and Thiocolchicoside in tablet dosage form by UV Spectrophotometric method

Kumari¹,

Chaudhary

Verma³

et al. 2022

RJPT

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A simple, accurate, precise, and rapid UV spectrophotometric method was developed for simultaneous estimation of Etoricoxib and Thiocolchicoside in the tablet dosage form. Etoricoxib showed maximum absorbance at 270 nm, while Thiocolchicoside showed maximum absorbance at 260 nm. These two wavelengths were selected for further analysis. The developed method was validated as ICH guidelines including parameters as linearity, range, precision, LOD, LOQ, and accuracy. The absorbance range for Etoricoxib (5-30 µg/ml) was found to be 0.210-0.799. These aliquots obeyed Beer-Lambert’s law with regression of 0.995. The absorbance range of Thiocolchicoside (5-30 µg/ml) was found to be 0.147-0.468 with a regression of 0.998. The method of precision (interday, intraday) was found within limits (RSD>2). The % recovery was found to be within the range. As per the ICH Q2 R1 guideline, the result was validated statistically and was found to be satisfactory.

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RP HPLC Method Development for Simultaneous Estimation of Etoricoxib and Thiocolchicoside

Cited by 3 publications

References 2 publications

Perspective Chapter: Numerical Simulation to Study Alfvén Waves

Perspective Chapter: Numerical Simulation to Study Alfvén Waves

Bioprospecting of serratiopeptidase-producing bacteria from different sources

Development and validation of an analytical method for the simultaneous estimation of Etoricoxib and Thiocolchicoside in tablet dosage form by UV Spectrophotometric method

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