Routine Prostaglandin Assay by GC-MS in Multiwell Tissue Culture Plates: Application to Human Synoviocytes and Chondrocytes

Knott, I.; Raes, Martine; Dieu, Marc; Lenoir, G.; Burton, Maggi; Remacle, Jean‐François

doi:10.1006/abio.1993.1208

Cited by 17 publications

(2 citation statements)

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“…This lineage plays a significant role in both joint inflammation and the destruction of joint tissue via formation of locally invasive pannus (14, 47). Consistent with another report that demonstrated that the FLS bear the synthetic machinery to produce PGE 2 , PGF 2 α, and PGI 2 (48), we find that FLS express high levels of PGI 2 synthase (Fig. 2A) in addition to both Cox-1 and Cox-2 (Fig.…”

Section: Resultssupporting

confidence: 92%

Platelets Participate in Synovitis via Cox-1–Dependent Synthesis of Prostacyclin Independently of Microparticle Generation

Boilard

Larabee

Shnayder

et al. 2011

The Journal of Immunology

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In addition to the well-described role of platelets in thrombosis, a growing body of evidence implicates platelets in diverse inflammatory responses. We recently showed platelets can contribute to the pathophysiology of inflammatory arthritis via IL-1–containing microparticles. In this study, we demonstrate that platelets, and not platelet microparticles, actively contribute to synovitis via production of proinflammatory prostacyclin in an autoimmune arthritis model. Using both genetic and pharmacologic approaches, we establish that paracrine production of prostacyclin proceeds in the absence of cyclooxygenase-2. Furthermore, we also demonstrate that prostacyclin generation can arise via transcellular collaboration between platelets and fibroblast-like synoviocytes. In addition to shedding light on an unappreciated pathway of lipid synthesis in arthritis, we further delineate a novel effector activity by which platelets can contribute to inflammatory disease.

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Section: Resultssupporting

confidence: 92%

Platelets Participate in Synovitis via Cox-1–Dependent Synthesis of Prostacyclin Independently of Microparticle Generation

Boilard

Larabee

Shnayder

et al. 2011

The Journal of Immunology

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“…PGI 2 and PGH 2 rearrange in aqueous solution to the stable metabolites 6-keto-PGF 1 ␣ , and prostaglandin E 2 (PGE 2 )/prostaglandin D2, respectively (30 …”

Section: Mass Spectroscopymentioning

confidence: 99%

The sterol response element binding protein regulates cyclooxygenase-2 gene expression in endothelial cells

Smith

Petrie

Morrow

et al. 2005

Journal of Lipid Research

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Arachidonic Acid Metabolism in Primary Irritant Dermatitis Produced by Patch Testing of Human Skin with Surfactants

Müller‐Decker

Heinzelmann

Fürstenberger

et al. 1998

Toxicology and Applied Pharmacology

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Routine Prostaglandin Assay by GC-MS in Multiwell Tissue Culture Plates: Application to Human Synoviocytes and Chondrocytes

Cited by 17 publications

References 0 publications

Platelets Participate in Synovitis via Cox-1–Dependent Synthesis of Prostacyclin Independently of Microparticle Generation

Platelets Participate in Synovitis via Cox-1–Dependent Synthesis of Prostacyclin Independently of Microparticle Generation

The sterol response element binding protein regulates cyclooxygenase-2 gene expression in endothelial cells

Arachidonic Acid Metabolism in Primary Irritant Dermatitis Produced by Patch Testing of Human Skin with Surfactants

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