Routine Identification of Yeasts with the Aid of Molybdate-Agar Medium

Bump, Charles M.; Kunz, Lawrence J.

doi:10.1128/aem.16.10.1503-1506.1968

Cited by 7 publications

(6 citation statements)

References 3 publications

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“…Costa and de Lourdes Branco (2) devised a phosphomolybdate agar on which C. albicans colonies are green and those of other species are blue. At least one other study confirmed the efficacy of this medium (1).…”

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confidence: 80%

CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species

1994

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is a novel, differential culture medium that is claimed to facilitate the isolation and presumptive identification of some clinically important yeast species. We evaluated the use of this medium with 726 yeast isolates, including 82 isolated directly on the medium from clinical material. After 2 days of incubation at 37°C, 285 C. albicans isolates gave distinctive green colonies that were not seen with any of 441 other yeast isolates representing 21 different species. A total of 54 C. tropicalis isolates also developed distinctive dark blue-gray colonies with a halo of dark brownish purple in the surrounding agar. C. krusei isolates (n = 43) also formed highly characteristic rough, spreading colonies with pale pink centers and a white edge that was otherwise encountered only rarely with isolates of C. norvegensis. Trichosporon spp. (n = 34) formed small, pale colonies that became larger and characteristically rough with prolonged incubation. Most of the other 310 yeasts studied formed colonies with a color that ranged from white to pink to purple with a brownish tint. The only exceptions were found among isolates identified as Geotrichum sp. or Pichia sp., some of which formed colonies with a gray to blue color and which in two instances formed a green pigment or a dark halo in the agar. The specificity and sensitivity of the new medium for the presumptive identification of C. albicans, C. krusei, and C. tropicalis exceeded 99%o for all three species. A blinded reading test involving four personnel and 57 yeast isolates representing nine clinically important species confirmed that colonial appearance after 48 h of incubation on CHROMagar Candida afforded the correct presumptive recognition of C. albicans, C. tropicalis, C. krusei, and Trichosporon spp. None of nine bacterial isolates grew on CHROMagar Candida within 72 h, and bacteria (Escherichia coli) grew from only 4 of 104 vaginal, 100 oral, and 99 anorectal swabs. The new medium supported the growth of 19 of 23 dermatophyte fungi tested and 41 of 43 other molds representing a broad range of fungal pathogens and contaminants. In parallel cultures of 348 clinical specimens set up on Sabouraud agar and CHROMagar Candida, both media grew yeasts in the same 78 instances. CHROMagar Candida is recommended as a useful isolation medium capable of the presumptive identification of the yeast species most commonly isolated from clinical material and facilitating recognition of mixed yeast cultures.

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confidence: 80%

CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species

1994

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“…Molybdate agar has been evaluated as a selective and differential medium for yeasts (2). The differential features of the medium were apparent in this study but not evaluated.…”

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confidence: 92%

Evaluation of Media for Selective Isolation of Yeasts from Oral, Rectal, and Burn Wound Specimens

Smith¹,

Blasi²,

Dayton³

1974

Applied Microbiology

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“…Approximately 50 % of these nonpathogenic Candida species are reactive with one or more of the selected factor sera. However, these saprophytic Candida species constitute less than 1% of all yeastlike fungi found in clinical material (2), and, because accuracy with the factor sera is comparable to that with conventional procedures (96%, Table 5), we do not consider the problem a formidable one.…”

Section: Discussionmentioning

confidence: 99%

“…A portion of a dissertation submitted by the first author to the University of North Carolina in partial fulfillment of the requirements for the degree of Doctor of Public Health in the School of Public Health. 2 Present address: Texas State Department of Health, Austin, Tex. 78756.…”

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Application of Agglutinins for the Rapid and Accurate Identification of Medically Important Candida Species 1

Sweet¹,

Kaufman²

1970

Applied Microbiology

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Agglutinins have been prepared against the medically important Candida species. Crude antisera to the various species demonstrated intense cross-reactions with heterologous yeastlike fungi as well as with many true yeasts. However, carefully monitored adsorptions of selected antisera allowed the production of six factor sera that proved useful in a slide agglutination test. These six sera permitted the rapid and specific identification of C. guilliermondii, C. krusei, C. parapsilosis, and C. pseudotropicalis. They also allowed the delineation of two groups: (i) C. albicans (type A)-C. tropicalis and (ii) C. albicans (type B)-C. stellatoidea. C. albicans type A could be readily distinguished from C. tropicalis by its ability to form germ tubes in serum. C. stellatoidea could be distinguished from C. albicans type B by its predominantly filamentous growth on a nutritionally deficient medium. The medically important Candida species could be identified within 24 hr by the combined use of serological and morphological procedures.

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Routine Identification of Yeasts with the Aid of Molybdate-Agar Medium

Cited by 7 publications

References 3 publications

CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species

CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species

Evaluation of Media for Selective Isolation of Yeasts from Oral, Rectal, and Burn Wound Specimens

Application of Agglutinins for the Rapid and Accurate Identification of Medically Important Candida Species 1

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