Rotavirus Infection in the Neonatal Nurseries of a Tertiary Care Hospital in India

Ramani, Sasirekha; Sowmyanarayanan, Thuppal V.; Gladstone, Beryl Primrose; Bhowmick, Kaushik; Asirvatham, Jaya Ruth; Jana, Atanu Kumar; Kuruvilla, K; Kumar, Manish; Gibikote, Sridhar; Kang, Gagandeep

doi:10.1097/inf.0b013e3181715170

Cited by 49 publications

(67 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Newborn babies can be infected in the nursery, but most will be asymptomatic or have mild disease. [39][40][41] Possible mechanisms for the mild or subclinical infections seen in newborn babies include the protective effect of circulating maternal antibodies, the relative immaturity of the neonatal gut and the reduced virulence properties of some unique neonatal strains. 41,42 Risk factors for severe rotavirus gastroenteritis include socioeconomic disadvantage, malnutrition, preterm birth, infants with low birth weight, compromised immunity and co-infection with other enteric pathogens.…”

Section: Introductionmentioning

confidence: 99%

“…[39][40][41] Possible mechanisms for the mild or subclinical infections seen in newborn babies include the protective effect of circulating maternal antibodies, the relative immaturity of the neonatal gut and the reduced virulence properties of some unique neonatal strains. 41,42 Risk factors for severe rotavirus gastroenteritis include socioeconomic disadvantage, malnutrition, preterm birth, infants with low birth weight, compromised immunity and co-infection with other enteric pathogens. [43][44][45][46] However, children with mild or asymptomatic human immunodeficiency virus (HIV) infections seem not to be at greater risk of severe illness than children without HIV, although they may shed rotaviruses for longer periods.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rotavirus vaccines: Opportunities and challenges

Grimwood¹,

Lambert²

2009

Human Vaccines

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rotavirus vaccines: Opportunities and challenges

Grimwood¹,

Lambert²

2009

Human Vaccines

View full text Add to dashboard Cite

mentioning

confidence: 99%

“…Surveillance studies have determined that G10P [11] RVs are a major cause of neonatal infection in Vellore, India, where the viruses have circulated for more than a decade (32,33). The persistence of G10P [11] RVs in Vellore may be due, in part, to their widespread contamination of the environment in neonatal nurseries, thus leading to nosocomial infections (33).…”

mentioning

confidence: 99%

“…The persistence of G10P [11] RVs in Vellore may be due, in part, to their widespread contamination of the environment in neonatal nurseries, thus leading to nosocomial infections (33). Unlike the asymptomatic infections caused by some neonatal RVs (e.g., RV3 and 116E), the Vellore viruses have been associated with GI disease in approximately half of the infected neonates (33). The Vellore G10P [11] viruses may not be entirely exclusive to neonates as G10P [11] strains have also been recovered, albeit sporadically, from older children with diarrheal illness (32).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Absence of Genetic Differences among G10P[11] Rotaviruses Associated with Asymptomatic and Symptomatic Neonatal Infections in Vellore, India

Libonati

Dennis

Ramani

et al. 2014

J Virol

Self Cite

View full text Add to dashboard Cite

Rotaviruses (RVs) are leading causes of severe diarrhea and vomiting in infants and young children. RVs with G10P[11] genotype specificity have been associated with symptomatic and asymptomatic neonatal infections in Vellore, India. To identify possible viral genetic determinants responsible for differences in symptomology, the genome sequences of G10P[11] RVs in stool samples of 19 neonates with symptomatic infections and 20 neonates with asymptomatic infections were determined by Sanger and next-generation sequencing. The data showed that all 39 viruses had identical genotype constellations (G10-P[11]-I2-R2-C2-M2-A1-N1-T1-E2-H3), the same as those of the previously characterized symptomatic N155 Vellore isolate. The data also showed that the RNA and deduced protein sequences of all the Vellore G10P[11] viruses were nearly identical; no nucleotide or amino acid differences were found that correlated with symptomatic versus asymptomatic infection. Next-generation sequencing data revealed that some stool samples, both from neonates with symptomatic infections and from neonates with asymptomatic infections, also contained one or more positive-strand RNA viruses (Aichi virus, astrovirus, or salivirus/klassevirus) suspected of being potential causes of pediatric gastroenteritis. However, none of the positive-strand RNA viruses could be causally associated with the development of symptoms. These results indicate that the diversity of clinical symptoms in Vellore neonates does not result from genetic differences among G10P[11] RVs; instead, other undefined factors appear to influence whether neonates develop gastrointestinal disease symptoms. IMPORTANCERotavirus (RV) strains have been identified that preferentially replicate in neonates, in some cases, without causing gastrointestinal disease. Surveillance studies have established that G10P[11] RVs are a major cause of neonatal infection in Vellore, India, with half of infected neonates exhibiting symptoms. We used Sanger and next-generation sequencing technologies to contrast G10P[11] RVs recovered from symptomatic and asymptomatic neonates. Remarkably, the data showed that the RNA genomes of the viruses were virtually indistinguishable and lacked any differences that could explain the diversity of clinical outcomes among infected Vellore neonates. The sequencing results also indicated that some symptomatic and some asymptomatic Vellore neonates were infected with other enteric viruses (Aichi virus, astrovirus, salvirus/klassevirus); however, none could be correlated with the presence of symptoms in neonates. Together, our findings suggest that other poorly defined factors, not connected to the genetic makeup of the Vellore G10P[11] viruses, influence whether neonates develop gastrointestinal disease symptoms.

show abstract

Surveillance for Vaccine‐Preventable Diseases and Immunization

Payne

2014

Concepts and Methods in Infectious Disease Surveillance

View full text Add to dashboard Cite

Rotavirus Infection in the Neonatal Nurseries of a Tertiary Care Hospital in India

Abstract: This study documents the high rates of rotavirus infection in the neonatal nurseries and the continuing detection of the G10P[11] strain associated with GI disease in Vellore.

Cited by 49 publications

References 32 publications

Rotavirus vaccines: Opportunities and challenges

Rotavirus vaccines: Opportunities and challenges

Absence of Genetic Differences among G10P[11] Rotaviruses Associated with Asymptomatic and Symptomatic Neonatal Infections in Vellore, India

Surveillance for Vaccine‐Preventable Diseases and Immunization

Contact Info

Product

Resources

About