Rotavirus genotyping: keeping up with an evolving population of human rotaviruses

Iturriza‐Gómara, Miren; Kang, Gagandeep; Gray, Jim

doi:10.1016/j.jcv.2004.04.009

Cited by 391 publications

(400 citation statements)

References 41 publications

Supporting

Mentioning

386

Contrasting

Unclassified

Order By: Relevance

“…The present study confirms the role of human rotavirus as an important enteropathogenic microbial agent and highlights the high prevalence of G1-G4 and G9 strains in Iranian paediatric patients. These findings are similar to epidemiological data described in Australia, Europe and North America, and differ from those observed in developing countries [7,22,23].…”

Section: Rotavirus Genotypingsupporting

confidence: 89%

Prevalence and Molecular Genotyping of Group A Rotaviruses in Iranian Children

Kargar

Akbarizadeh

2012

Indian J. Virol.

View full text Add to dashboard Cite

Rotavirus is the leading cause of acute gastroenteritis in worldwide young children. Effective vaccines to prevent rotavirus infection are currently available, although their clinical use is still limited, and rotavirus still causes many episodes of infantile gastroenteritis, mainly during the winter season. The aim of this study was to evaluate the prevalence of rotavirus infection in children aged \5-years-old who were hospitalised for gastroenteritis. One hundred and sixty-three stool samples from hospitalised children (\5-years-old) complicated with severe diarrhoea, in two hospitals in Jahrom City, Iran were collected from 2009 to 2010. Antigenic prevalence of rotavirus group A was distinguished by enzyme immunoassay. The antigen of group A rotavirus was diagnosed by EIA in 75 of 163 collected samples. The genotype of EIA-positive samples was determined by nested RT-PCR. The frequency of rotavirus genotypes G1, G2, G3, G4 and G9 was 17.33, 13.34, 2.67, 30.66 and 2.67 %, respectively. Also, the frequency of mixed and non-typable genotypes was detected in 2.67 and 30.66 %, respectively. G1/G8 mixed infection was the first of these rotavirus genotypes to be reported in Iran. Detection of high prevalence of group A rotavirus infection in hospitalised children with diarrhoea, and determination of circulating rotavirus genotypes in this region of Iran, provide useful data for formulating effective vaccines; especially for infants less than 5-years-old.

show abstract

Section: Rotavirus Genotypingsupporting

confidence: 89%

Prevalence and Molecular Genotyping of Group A Rotaviruses in Iranian Children

Kargar

Akbarizadeh

2012

Indian J. Virol.

View full text Add to dashboard Cite

show abstract

“…Positive and negative controls were also included. One-step semi-nested multiplex reverse transcriptase polymerase chain reaction (RT-PCR) using SuperScript One-Step RT-PCR with Platinum ® Taq (Invitrogen, USA) and one set of primers described by Das , a random primed reverse transcription step followed by a two-step PCR procedure was implemented where the consensus primers VP7-F/VP7-R and VP4-F/VP4-R were used for the first PCR reactions for G and P typing, respectively, while the second PCR assays were performed by using mixes of one consensus and type-specific primers as follows: for G genotype determination (VP7-R and aBT1/ G1, aCT2/G2, mG3/G3, aDT4/G4, aAT8/G8, mG9/G9, G10, G12) [23,31,32], and for P genotype detection (VP4-F and 1 T-1D, 2 T-1, 3 T-1, 4 T-1, 5 T-1, P [11]) [30,33,34].…”

Section: G and P Genotypingmentioning

confidence: 99%

Molecular epidemiology of rotaviruses in Bulgaria: annual shift of the predominant genotype

Mladenova

Korsun²,

Geonova³

et al. 2010

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

show abstract

“…Samples with remaining material in which EIA provided the highest optical density (>2000) were suspended in phosphate buffered saline or B199 medium (Sigma-Aldrich, St Louis MO, USA), frozen at −80°C and subsequently analyzed for G-and P-type through multiplex, reverse transcription (RT)-polymerase chain reaction (PCR) methods [10] from 2006 onwards. Viral RNA was obtained with an automatic BioRobot® M48 extractor (Qiagen GmbH, Hilden, Germany) using the MagAttact® Virus Mini M48 kit or the NucliSENS® Easy-Mag platform (bio-Mèrieux SA, Marcy l'Etoile, France).…”

Section: Virological Studiesmentioning

confidence: 99%

“…Given the variety of existing rotavirus genotypes, the frequency with which they undergo genetic reassortment and the occasional transmission of animal rotaviruses to humans [9,10], circulating genotypes must be monitored to detect the emergence of new or unusual strains that could spread geographically and compromise the effectiveness of vaccination programs [5,9]. This article presents the results of rotavirus G-and P-typing over a 13-year period in children in the Basque Country (Spain).…”

Section: Introductionmentioning

confidence: 99%