Rotavirus diversity among diarrheal children in Delhi, India during 2007–2012

Tiku, Vasundhara Razdan; Sharma, Sumit; Kumar, Praveen; Raghavendhar, Siva; Aneja, Satinder; Paul, Vinod K.; Bhan, Maharaj Kishan; Ray, Pratima

doi:10.1016/j.vaccine.2014.03.005

Cited by 36 publications

(27 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A written informed consent was obtained from one of the parents of the child before enrollment. All (350) children with diarrhea of less than 3 days duration were screened for group A rotavirus [17]. The remaining group A rotavirus (RV) negative (180) fecal specimens were stored in a deep freezer (−80 °C) for further testing.…”

Section: Methodsmentioning

confidence: 99%

First study conducted in Northern India that identifies group C rotavirus as the etiological agent of severe diarrhea in children in Delhi

Tiku

Jiang

Kumar

et al. 2017

Virol J

Self Cite

View full text Add to dashboard Cite

BackgroundGroup C Rotavirus (RVC) is an enteric pathogen responsible for acute gastroenteritis in children and adults globally. At present there are no surveillance studies on group C Rotaviruses in India and therefore their prevalence in India remains unknown. The present study aimed to evaluate group C rotavirus infection among <5 years old children hospitalized with acute gastroenteritis in New Delhi.MethodsA total of 350 fecal specimens were collected during September 2013 to November 2014 from <5 years old diarrheal patients admitted at KSCH hospital, Delhi. The samples found negative for group A rotavirus (N = 180) by Enzyme immunoassay were screened for group C rotavirus by RT-PCR with VP6, VP7 and VP4 gene specific primers. The PCR products were further sequenced (VP6, VP7, VP4) and analyzed to ascertain their origin and G and P genotypes.ResultsSix out of 180 (group A rotavirus negative) samples were found positive for group C rotavirus by VP6 gene specific RT-PCR, of which 3 were also found positive for VP7 and VP4 genes. Phylogenetic analysis of VP7 and VP4 genes of these showed them to be G4 and P[2] genotypes. Overall, the nucleotide sequence data (VP6, VP7 and VP4) revealed a close relationship with the human group C rotavirus with no evidence of animal ancestry. Interestingly, the nucleotide sequence analysis of various genes also indicated differences in their origin. While the identity matrix of VP4 gene (n = 3) showed high amino acid sequence identity (97.60 to 98.20%) with Korean strain, the VP6 gene (n = 6) showed maximum identity with Nigerian strain (96.40 to 97.60%) and VP7 gene (n = 3) with Bangladeshi and USA strains. This is true for all analyzed samples.ConclusionOur study demonstrated the group C rotavirus as the cause of severe diarrhea in young children in Delhi and provides insights on the origin of group C rotavirus genes among the local strains indicating their source of transmission. Our study also highlights the need for a simple and reliable diagnostic test that can be utilized to determine the disease burden due to group C rotavirus in India.

show abstract

Section: Methodsmentioning

confidence: 99%

First study conducted in Northern India that identifies group C rotavirus as the etiological agent of severe diarrhea in children in Delhi

Tiku

Jiang

Kumar

et al. 2017

Virol J

Self Cite

View full text Add to dashboard Cite

show abstract

“…These RVA G9P[8] strains have been under circulation and found to cause symptomatic infections among children <5 years of age, who were expected to be protected by the pre‐existing immunity which would provide heterotypic protection against common genotypes . Over the years, G9 RVA strains are under circulation worldwide including India …”

Section: Introductionmentioning

confidence: 99%

“…24 Over the years, G9 RVA strains are under circulation worldwide including India. [25][26][27][28][29] In India, surveillance studies conducted on rotavirus disease and the genotype distribution of these strains reported over the years have revealed that G9 RVA genotype is circulating since 1994 in different geographical regions. 26,[28][29][30][31] Recent studies have documented proportion of RVA infections with G9 strains at 13-40% level in acute gastroenteritis.…”

Section: Introductionmentioning

confidence: 99%

“…[25][26][27][28][29] In India, surveillance studies conducted on rotavirus disease and the genotype distribution of these strains reported over the years have revealed that G9 RVA genotype is circulating since 1994 in different geographical regions. 26,[28][29][30][31] Recent studies have documented proportion of RVA infections with G9 strains at 13-40% level in acute gastroenteritis. 26,28,29,32 Increase in the circulation of G9P [8] RV strains has also been reported during the study period from western and northern India.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Full genome analysis of rotavirus G9P[8] strains identified in acute gastroenteritis cases reveals genetic diversity: Pune, western India

Tatte

Chaphekar

Gopalkrishna

2017

Journal of Medical Virology

View full text Add to dashboard Cite

Group A rotaviruses (RVA) are the major enteric etiological agents of severe acute gastroenteritis among children globally. As G9 RVA now represents as one of the major human RVA genotypes, studies on full genome of this particular genotype are being carried out worldwide. So far, no such studies on G9P[8] RVAs have been reported from Pune, western part of India. Keeping in view of this, the study was undertaken to understand the degree of genetic diversity of the commonly circulating G9P[8] RVA strains. Rotavirus surveillance studies carried out earlier during the years 2009-2011 showed increase in the prevalence of G9P[8] RVAs. Representative G9P[8] RVA strains from the years 2009, 2010, and 2011 were selected for the study. In general, all the G9 RVA strains showed clustering in the globally circulating sublineage of the VP7 gene and showed nucleotide/amino acid identities of 96.8-99.7%/96.9-99.8% with global G9 RV strains. Full genome analysis, of all three RVAs in this study indicated Wa-like genotype constellation G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. Within the strains nucleotide/amino acid divergence of 0.1-3.4%/0.0-4.1% was noted in all the RVA structural and non-structural genes. In conclusion, the present study highlights intra-genotypic variations throughout the RVA genome. The study further emphasizes the need for surveillance and analysis of the whole genomic constellation of the commonly circulating RVA strains of other regions in the country for understanding to a greater degree of the impact of rotavirus vaccination recently introduced in India.

show abstract

“…For children under 5 years of age, 1700 000 000 episodes of diarrhea are seen worldwide, and death occurs in 700 000 of these cases due to diarrhea . Furthermore, rotavirus is an important cause of diarrhea in this age group . Vitamin D is a fat‐soluble vitamin that is produced in the skin when sunlight is absorbed.…”

mentioning

confidence: 99%